Spastin (SPAST) is a 616-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9UBP0.
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The mean pLDDT of this model is 76.0 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 46% |
| 70 to 90 | Confident: backbone generally right | 21% |
| 50 to 70 | Low: treat with caution | 10% |
| Below 50 | Very low: often disordered regions | 23% |
What pLDDT means and how to read it
ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:11809724, PubMed:15716377, PubMed:16219033, PubMed:17389232, PubMed:20530212, PubMed:22637577, PubMed:26875866). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed:26875866). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules…
Homohexamer (PubMed:17389232, PubMed:22637577). Mostly monomeric, but assembles into hexameric structure for short periods of time. Oligomerization seems to be a prerequisite for catalytic activity (PubMed:17389232, PubMed:22637577). Binding to ATP in a cleft between two adjacent subunits stabilizes the homohexameric form (PubMed:17389232, PubMed:22637577). Binds to microtubules at least in part…
Membrane, Endoplasmic reticulum, Midbody, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, Cytoplasm, perinuclear region, Nucleus, Cytoplasm, cytoskeleton, spindle, Cytoplasm, Cell projection, axon, Endoplasmic reticulum membrane, Nucleus membrane, Lipid…
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 7S7J | X-ray | 1.15 Å | A=112-195 |
| 3EAB | X-ray | 2.5 Å | A/B/C/D/E/F=112-196 |
| 5Z6Q | X-ray | 3.0 Å | A=229-616 |
| 5Z6R | X-ray | 3.0 Å | A=229-616 |
| 3VFD | X-ray | 3.3 Å | A=228-616 |
| 6PEK | EM | 4.2 Å | A/B/C/D/E=87-616 |
| 6PEN | EM | 4.2 Å | A/B/C/D/E/F=87-616 |
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