1B86: Protein

Human deoxyhaemoglobin-2,3-diphosphoglycerate complex. Determined by X-ray diffraction at 2.5 Å resolution. Released 14 Feb 1999.

Method: X-ray diffraction
Resolution: 2.5 Å
Organism: Homo sapiens
Chains: 4
Atoms: 4,715
Mol. weight: 64.88 kDa
Ligands: DG2, OXY, HEM
Released: 14 Feb 1999

Explore 1B86 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1B86 contains 45 α-helices and 0 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	4-17	14
α-helix	18-20	3
α-helix	21-35	15
α-helix	37-42	6
α-helix	53-71	19
α-helix	76-79	4
α-helix	81-86	6
α-helix	87-91	5
α-helix	95-97	3
α-helix	98-112	15
α-helix	119-136	18

Chain B: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	150-158	9
α-helix	163-177	15
α-helix	179-184	6
α-helix	186-188	3
α-helix	194-198	5
α-helix	201-218	18
α-helix	224-233	10
α-helix	234-238	5
α-helix	244-261	18
α-helix	262-264	3
α-helix	267-285	19

Chain C: 12 helices, 0 β-strands

Element	Residues	Length
α-helix	404-415	12
α-helix	418-420	3
α-helix	421-435	15
α-helix	437-442	6
α-helix	453-471	19
α-helix	476-479	4
α-helix	481-486	6
α-helix	487-491	5
α-helix	495-497	3
α-helix	498-510	13
α-helix	519-536	18
α-helix	537-539	3

Chain D: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	548-558	11
α-helix	563-577	15
α-helix	579-584	6
α-helix	586-588	3
α-helix	594-599	6
α-helix	601-618	18
α-helix	624-634	11
α-helix	635-639	5
α-helix	644-661	18
α-helix	662-664	3
α-helix	667-684	18

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein (hemoglobin; alpha chain)	A, C	protein	141	Homo sapiens	P69905 (AlphaFold model)
Protein (hemoglobin; beta chain)	B, D	protein	146	Homo sapiens	P68871 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>1B86_1 PROTEIN (HEMOGLOBIN; ALPHA CHAIN) (chains A, C)
VLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGK
KVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPA
VHASLDKFLASVSTVLTSKYR

Sequence of entity 2 (B, D), FASTA

>1B86_2 PROTEIN (HEMOGLOBIN; BETA CHAIN) (chains B, D)
VHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKV
KAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGK
EFTPPVQAAYQKVVAGVANALAHKYH

Ligands and cofactors

ID	Name	Formula	Copies
DG2	(2R)-2,3-diphosphoglyceric acid	C3 H8 O10 P2	1
OXY	Oxygen molecule	O2	2
HEM	Protoporphyrin IX containing FE	C34 H32 Fe N4 O4	4

Primary citation

Human deoxyhaemoglobin-2,3-diphosphoglycerate complex low-salt structure at 2.5 A resolution. Richard, V., Dodson, G.G., Mauguen, Y. J Mol Biol (1993) 233:270-274. DOI 10.1006/jmbi.1993.1505 · PubMed

Other PDB entries of the same protein (UniProt P69905 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2W72 1.07 Å, Deoxygenated structure of a distal site hemoglobin mutant plus xe
1IRD 1.25 Å, Crystal Structure of Human Carbonmonoxy-Haemoglobin at 1.25 A Resolution
2DN1 1.25 Å, 1.25A resolution crystal structure of human hemoglobin in the oxy form
2DN2 1.25 Å, 1.25A resolution crystal structure of human hemoglobin in the deoxy form
2DN3 1.25 Å, 1.25A resolution crystal structure of human hemoglobin in the carbonmonoxy form
7DY4 1.3 Å, High resolution crystal structure of hemoglobin M Boston.
6KA9 1.4 Å, Crosslinked alpha(Fe-CO)-beta(Ni) human hemoglobin A in the T quaternary structure at 95…
6KAO 1.4 Å, Carbonmonoxy human hemoglobin C in the R quaternary structure at 95 K: Dark
6LCW 1.4 Å, Crosslinked alpha(Ni)-beta(Ni) human hemoglobin A in the T quaternary structure at 95 K:…
6LCX 1.4 Å, Crosslinked alpha(Ni)-beta(Ni) human hemoglobin A in the T quaternary structure at 95 K:…
7DY3 1.4 Å, High resolution crystal structure of hemoglobin M Iwate.
3S66 1.4 Å, Structures and oxygen affinities of crystalline human hemoglobin C (beta6 Lys) in the R…

Browse more

1B86 is part of these collections:

Hemoglobin and oxygen transport

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