1FBV: Signal transduction protein cbl

Name: PDB 1FBV: Structure of a cbl-UBCH7 complex: ring domain function in ubiquitin-protein ligases
Published: 2000-08-30
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: ligase

Structure of a cbl-UBCH7 complex: ring domain function in ubiquitin-protein ligases. Determined by X-ray diffraction at 2.9 Å resolution. Released 30 Aug 2000.

Method: X-ray diffraction
Resolution: 2.9 Å
Organism: Homo sapiens
Chains: 3
Atoms: 4,393
Mol. weight: 64.33 kDa
Ligands: ZN
Released: 30 Aug 2000

Explore 1FBV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1FBV contains 31 α-helices and 16 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	48-50	3
α-helix	53-70	18
α-helix	73-75	3
α-helix	84-101	18
α-helix	106-110	5
α-helix	113-135	23
α-helix	138-140	3
α-helix	146-168	23
α-helix	170-172	3
α-helix	176-178	3
α-helix	184-194	11
β-strand	199-201	3	1
α-helix	202-212	11
α-helix	218-228	11
β-strand	235-237	3	1
α-helix	238-247	10
α-helix	251-253	3
α-helix	254-258	5
α-helix	259-263	5
β-strand	268	1	2
α-helix	274-281	8
α-helix	282-284	3
β-strand	290-295	6	2
β-strand	303-308	6	2
β-strand	314-317	4	2
α-helix	324-333	10
β-strand	339-340	2	2
α-helix	359-362	4
α-helix	365-372	8
β-strand	380	1	3
β-strand	388	1	3
β-strand	391-392	2	4
β-strand	399-400	2	4
α-helix	402-410	9
α-helix	431-433	3

Chain C: 7 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	1007-1016	10
β-strand	1022-1026	5	5
β-strand	1034-1039	6	5
β-strand	1052-1056	5	5
β-strand	1067-1070	4	5
β-strand	1084	1	5
α-helix	1088-1090	3
α-helix	1101-1104	4
α-helix	1109-1112	4
α-helix	1123-1126	4
α-helix	1133-1138	6
α-helix	1143-1146	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Signal transduction protein cbl	A	protein	388	Homo sapiens	P22681 (AlphaFold model)
Zap-70 peptide	B	protein	9		P43403 (AlphaFold model)
Ubiquitin-conjugating enzyme E12-18 kda UBCH7	C	protein	154	Homo sapiens	P68036 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1FBV_1 SIGNAL TRANSDUCTION PROTEIN CBL (chains A)
PPGTVDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKM
ETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELK
GIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGLEAMALKS
TIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIH
KPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQN
PDLTGLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLT
SWQESEGQGCPFCRCEIKGTEPIVVDPF

Sequence of entity 2 (B), FASTA

>1FBV_2 ZAP-70 PEPTIDE (chains B)
SDGYTPEPA

Sequence of entity 3 (C), FASTA

>1FBV_3 UBIQUITIN-CONJUGATING ENZYME E12-18 KDA UBCH7 (chains C)
MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAE
YPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHP
LRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	2

Water and common crystallization additives (SO4) are not listed.

Primary citation

Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases. Zheng, N., Wang, P., Jeffrey, P.D. et al. Cell (2000) 102:533-539. DOI 10.1016/S0092-8674(00)00057-X · PubMed

Other PDB entries of the same protein (UniProt P22681 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3BUX 1.35 Å, Crystal structure of c-Cbl-TKB domain complexed with its binding motif in c-Met
3BUW 1.45 Å, Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Syk
7SIY 1.48 Å, cCBL TKB domain in complex with pZAP70 peptide
5HKY 1.8 Å, Crystal structure of c-Cbl TKBD domain in complex with SPRY2 peptide (36-60, pY55)…
5HKZ 1.8 Å, Crystal Structure of c-Cbl TKBD in complex with SPRY2 peptide (36-60, pY55) Refined to…
5HKX 1.85 Å, Crystal Structure of c-Cbl TKBD-RING domains (Y371E mutant) Refined to 1.85 A Resolution
3PLF 1.92 Å, Reverse Binding Mode of MetRD peptide complexed with c-Cbl TKB domain
2Y1N 2.0 Å, Structure of c-Cbl-ZAP-70 peptide complex
3BUM 2.0 Å, Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty2
3BUN 2.0 Å, Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty4
9ERZ 2.02 Å, Structure of CBL-TKBD bound to Ubiquitin-fused CBLock peptide
1YVH 2.05 Å, Crystal Structure of the c-Cbl TKB Domain in Complex with the APS pTyr-618 Phosphopeptide

Browse structure collections

About this viewer

MolViewer shows 1FBV directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.