1MF8: Human calcineurin

Crystal Structure of human calcineurin complexed with cyclosporin A and human cyclophilin. Determined by X-ray diffraction at 3.1 Å resolution. Released 16 Oct 2002.

Method: X-ray diffraction
Resolution: 3.1 Å
Organisms: HOMO SAPIENS, TOLYPOCLADIUM INFLATUM
Chains: 4
Atoms: 5,522
Mol. weight: 81.61 kDa
Ligands: PO4, CA
Released: 16 Oct 2002

Explore 1MF8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1MF8 contains 32 α-helices and 43 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	29	1	1
α-helix	31-34	4
β-strand	35	1	2
β-strand	41	1	2
α-helix	43-50	8
β-strand	56	1	1
α-helix	58-74	17
β-strand	78-81	4	3
α-helix	82	1
β-strand	85-88	4	4
α-helix	95-105	11
β-strand	112-115	4	4
α-helix	126-139	14
β-strand	146	1	4
α-helix	154-159	6
α-helix	162-169	8
α-helix	172-182	11
β-strand	188-191	4	3
β-strand	195-197	3	3
β-strand	203	1	5
β-strand	206	1	5
α-helix	209-214	6
α-helix	221-222	2
α-helix	226-232	7
β-strand	234-235	2	6
β-strand	249-250	2	6
β-strand	258-260	3	6
α-helix	262-271	10
β-strand	276-279	4	3
β-strand	288-290	3	3
β-strand	293	1	7
β-strand	300	1	7
β-strand	302-305	4	3
α-helix	311-313	3
α-helix	317-318	2
β-strand	319-325	7	4
β-strand	328-334	7	4
α-helix	344-346	3
α-helix	349-369	21

Chain B: 12 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	16-29	14
β-strand	36-37	2	8
α-helix	39-42	4
α-helix	46-48	3
α-helix	54-61	8
β-strand	69-70	2	8
α-helix	71-78	8
α-helix	79-81	3
α-helix	87-98	12
β-strand	105-106	2	9
α-helix	108-119	12
α-helix	125-139	15
β-strand	147-148	2	9
α-helix	149-156	8
α-helix	157-159	3
α-helix	161-163	3

Chain C: 3 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	5-12	8	10
β-strand	15-24	10	10
α-helix	30-41	12
β-strand	52	1	10
β-strand	55-57	3	10
β-strand	61-64	4	10
β-strand	77	1	11
β-strand	80	1	11
β-strand	83	1	12
β-strand	97-100	4	10
β-strand	102	1	13
β-strand	108	1	12
β-strand	112-115	4	10
α-helix	120-122	3
β-strand	123	1	14
β-strand	126	1	14
β-strand	128-134	7	10
α-helix	136-143	8
β-strand	156-163	8	10

Chain D: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	5	1	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Calmodulin-dependent calcineurin a subunit, alpha isoform	A	protein	373	HOMO SAPIENS	Q08209 (AlphaFold model)
Calcineurin B subunit isoform 1	B	protein	170	HOMO SAPIENS	P63098 (AlphaFold model)
Peptidyl-prolyl cis-trans isomerase a	C	protein	165	HOMO SAPIENS	P62937 (AlphaFold model)
Cyclosporin a	D	protein	11	TOLYPOCLADIUM INFLATUM

Sequence of entity 1 (A), FASTA

>1MF8_1 CALMODULIN-DEPENDENT CALCINEURIN A SUBUNIT, ALPHA ISOFORM (chains A)
AVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLL
DIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKIL
YPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVH
GGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYF
YSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKA
AVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGS
EEDGFDGATAAAR

Sequence of entity 2 (B), FASTA

>1MF8_2 CALCINEURIN B SUBUNIT ISOFORM 1 (chains B)
MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVID
IFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMV
GNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV

Sequence of entity 3 (C), FASTA

>1MF8_3 PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A (chains C)
MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGF
MCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTE
WLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE

Sequence of entity 4 (D), FASTA

>1MF8_4 CYCLOSPORIN A (chains D)
ALLVTAGLVLA

Ligands and cofactors

ID	Name	Formula	Copies
PO4	Phosphate ion	O4 P	1
CA	Calcium ion	Ca	4

Primary citation

Crystal Structure of Human Calcineurin Complexed with Cyclosporin a and Human Cyclophilin. Jin, L., Harrison, S.C. Proc Natl Acad Sci U S A (2002) 99:13522. DOI 10.1073/PNAS.212504399 · PubMed

Other PDB entries of the same protein (UniProt Q08209 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2W73 1.45 Å, High-resolution structure of the complex between calmodulin and a peptide from…
4F0Z 1.7 Å, Crystal Structure of Calcineurin in Complex with the Calcineurin-Inhibiting Domain of…
6UUQ 1.85 Å, Structure of Calcineurin bound to RCAN1
2R28 1.86 Å, The complex Structure of Calmodulin Bound to a Calcineurin Peptide
6NUC 1.9 Å, Structure of Calcineurin in complex with NHE1 peptide
6NUF 1.9 Å, Structure of Calcineurin in complex with NHE1 peptide
4Q5U 1.95 Å, Structure of calmodulin bound to its recognition site from calcineurin
3LL8 2.0 Å, Crystal Structure of Calcineurin in Complex with AKAP79 Peptide
9NXE 2.09 Å, Crystal Structure of Calcineurin Clinical Variant E282K
1AUI 2.1 Å, Human calcineurin heterodimer
9NXN 2.1 Å, Crystal structure of CN:RII alpha
2P6B 2.3 Å, Crystal Structure of Human Calcineurin in Complex with PVIVIT Peptide

Browse structure collections

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