1TFX: Trypsin

Complex of the second kunitz domain of tissue factor pathway inhibitor with porcine trypsin. Determined by X-ray diffraction at 2.6 Å resolution. Released 21 Jan 1998.

Method: X-ray diffraction
Resolution: 2.6 Å
Organisms: Sus scrofa, Homo sapiens
Chains: 4
Atoms: 4,383
Mol. weight: 60.77 kDa
Ligands: CA
Released: 21 Jan 1998

Explore 1TFX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1TFX contains 26 α-helices and 46 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	1
β-strand	20-21	2	2
α-helix	22-23	2
β-strand	30-34	5	3
β-strand	40-48	9	3
β-strand	51-54	4	3
α-helix	56-58	3
β-strand	64-67	4	3
β-strand	72	1	4
β-strand	81-90	10	3
β-strand	104-108	5	3
β-strand	122	1	2
α-helix	123-124	2
α-helix	128-130	3
β-strand	135-140	6	2
β-strand	154	1	4
β-strand	156-162	7	2
α-helix	163-164	2
α-helix	165-171	7
β-strand	180-183	4	2
β-strand	189	1	1
β-strand	198-201	4	2
β-strand	204-215	8	2
α-helix	225	1
β-strand	226-230	5	2
α-helix	231-234	4
α-helix	235-242	8

Chain B: 9 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	5
β-strand	20-21	2	6
α-helix	22-23	2
β-strand	30-34	5	7
β-strand	40-48	9	7
β-strand	51-54	4	7
α-helix	56-58	3
β-strand	64-67	4	7
β-strand	72	1	8
β-strand	81-90	10	7
β-strand	104-108	5	7
α-helix	111-113	3
β-strand	122	1	6
α-helix	123-124	2
α-helix	128-130	3
β-strand	135-140	6	6
β-strand	154	1	8
β-strand	156-162	7	6
α-helix	163-164	2
α-helix	165-171	7
β-strand	180-183	4	6
β-strand	189	1	5
β-strand	198-201	4	6
β-strand	204-215	8	6
β-strand	221B	1	9
β-strand	224	1	9
β-strand	226-230	5	6
α-helix	231-234	4
α-helix	235-244	10

Chain C: 4 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	3-6	4
α-helix	8-9	2
β-strand	14	1	2
β-strand	18-24	7	10
β-strand	29-35	7	10
β-strand	45	1	10
α-helix	48-51	4
α-helix	52-57	6

Chain D: 4 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	3-6	4
α-helix	8-9	2
β-strand	14	1	6
β-strand	18-24	7	11
β-strand	29-35	7	11
β-strand	45	1	11
α-helix	48-51	4
α-helix	52-56	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Trypsin	A, B	protein	223	Sus scrofa	P00761 (AlphaFold model)
Tissue factor pathway inhibitor	C, D	protein	58	Homo sapiens	P10646 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>1TFX_1 TRYPSIN (chains A, B)
IVGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNIDVLEG
NEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCAAAGTECLISG
WGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGFLEGGKDSCQGDSGGP
VVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWIQQTIAAN

Sequence of entity 2 (C, D), FASTA

>1TFX_2 TISSUE FACTOR PATHWAY INHIBITOR (chains C, D)
KPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDG

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	2

Primary citation

The second Kunitz domain of human tissue factor pathway inhibitor: cloning, structure determination and interaction with factor Xa. Burgering, M.J., Orbons, L.P., van der Doelen, A. et al. J Mol Biol (1997) 269:395-407. DOI 10.1006/jmbi.1997.1029 · PubMed

Other PDB entries of the same protein (UniProt P00761 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9PDA 1.18 Å, Structure of Porcine Trypsin Crystals Grown From PEG and Complexed With Crystallization…
1S83 1.25 Å, Porcine trypsin complexed with 4-amino propanol
2A31 1.25 Å, Trypsin in complex with borate
9PDC 1.27 Å, Porcine Trypsin grown from PEG and Complexed with Crystallization Additives II
9PD9 1.28 Å, The Structure of Porcine Trypsin in Complex with Crystallization Additives I
1S5S 1.4 Å, Porcine trypsin complexed with guanidine-3-propanol inhibitor
9PCT 1.41 Å, Structure of Porcine Trypsin Crystals Grown from PEG Complexed with Crystallization…
1S6H 1.45 Å, Porcine trypsin complexed with guanidine-3-propanol inhibitor
1H9H 1.5 Å, Complex of eeti-II with porcine trypsin
2A32 1.5 Å, Trypsin in complex with benzene boronic acid
1FNI 1.6 Å, Crystal structure of porcine beta trypsin with 0.01% polydocanol
1MCT 1.6 Å, The refined 1.6 Å resolution crystal structure of the complex formed between porcine…

Browse structure collections

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