1XCG: Human RhoA

Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF. Determined by X-ray diffraction at 2.5 Å resolution. Released 14 Dec 2004.

Method: X-ray diffraction
Resolution: 2.5 Å
Organism: Homo sapiens
Chains: 4
Atoms: 8,750
Mol. weight: 125.88 kDa
Released: 14 Dec 2004

Explore 1XCG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1XCG contains 58 α-helices and 33 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	725-727	3
α-helix	730-755	26
α-helix	756-761	6
α-helix	762-766	5
α-helix	772-778	7
α-helix	782-801	20
α-helix	810-817	8
α-helix	819-833	15
α-helix	836-849	14
α-helix	851-861	11
α-helix	864-866	3
α-helix	871-874	4
α-helix	877-894	18
α-helix	901-939	39
β-strand	940-941	2	1
α-helix	943-946	4
α-helix	954-958	5
α-helix	961-963	3
β-strand	966-974	9	1
β-strand	983-989	7	1
β-strand	992-998	7	1
β-strand	1003-1004	2	1
β-strand	1025-1027	3	1
α-helix	1028-1030	3
β-strand	1031-1035	5	1
β-strand	1042-1047	6	1
β-strand	1056-1060	5	1
α-helix	1064-1080	17

Chain B: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	4-11	8	2
α-helix	18-27	10
β-strand	42-48	7	2
β-strand	51-58	8	2
α-helix	70-73	4
β-strand	79-85	7	2
α-helix	89-94	6
α-helix	95-99	5
α-helix	100-106	7
β-strand	112-117	6	2
α-helix	119-121	3
α-helix	125-133	9
α-helix	141-150	10
β-strand	155-158	4	2
α-helix	167-179	13

Chain E: 20 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	716-718	3
α-helix	725-727	3
α-helix	730-755	26
α-helix	756-761	6
α-helix	762-766	5
α-helix	772-778	7
α-helix	782-801	20
α-helix	810-817	8
α-helix	820-833	14
α-helix	836-849	14
α-helix	851-861	11
α-helix	864-866	3
α-helix	871-874	4
α-helix	877-894	18
α-helix	901-937	37
β-strand	940-941	2	3
α-helix	951-954	4
α-helix	961-963	3
β-strand	966-977	12	3
β-strand	980-989	10	3
β-strand	992-998	7	3
β-strand	1003-1004	2	3
β-strand	1008	1	4
β-strand	1022	1	4
β-strand	1025-1027	3	3
α-helix	1028-1030	3
β-strand	1031-1035	5	3
β-strand	1042-1047	6	3
α-helix	1053-1054	2
β-strand	1055-1060	6	3
α-helix	1064-1080	17

Chain F: 10 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	4-11	8	5
α-helix	18-26	9
β-strand	42-48	7	5
β-strand	51-58	8	5
α-helix	70-73	4
β-strand	79-85	7	5
β-strand	88	1	5
α-helix	89-94	6
α-helix	95-99	5
α-helix	100-106	7
β-strand	112-117	6	5
α-helix	119-121	3
α-helix	125-132	8
α-helix	138-140	3
α-helix	141-150	10
β-strand	155-158	4	5
α-helix	167-179	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Rho guanine nucleotide exchange factor 11	A, E	protein	368	Homo sapiens	O15085 (AlphaFold model)
Transforming protein RhoA	B, F	protein	178	Homo sapiens	P61586 (AlphaFold model)

Sequence of entity 1 (A, E), FASTA

>1XCG_1 Rho guanine nucleotide exchange factor 11 (chains A, E)
QNWQHTVGKDVVAGLTQREIDRQEVINELFVTEASHLRTLRVLDLIFYQRMKKENLMPRE
ELARLFPNLPELIEIHNSWCEAMKKLREEGPIIKEISDLMLARFDGPAREELQQVAAQFC
SYQSIALELIKTKQRKESRFQLFMQEAESHPQCRRLQLRDLIISEMQRLTKYPLLLESII
KHTEGGTSEHEKLCRARDQCREILKYVNEAVKQTENRHRLEGYQKRLDATALERASNPLA
AEFKSLDLTTRKMIHEGPLTWRISKDKTLDLHVLLLEDLLVLLQKQDEKLLLKCHSKTAV
GSSDSKQTFSPVLKLNAVLIRSVATDKRAFFIICTSKLGPPQIYELVALTSSDKNTWMEL
LEEAVRNA

Sequence of entity 2 (B, F), FASTA

>1XCG_2 Transforming protein RhoA (chains B, F)
AIRKKLVIVGDGACGKTCLLIVNSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAG
QEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLR
NDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQ

Primary citation

The crystal structure of RhoA in complex with the DH/PH fragment of PDZRhoGEF, an activator of the Ca(2+) sensitization pathway in smooth muscle. Derewenda, U., Oleksy, A., Stevenson, A.S. et al. Structure (2004) 12:1955-1965. DOI 10.1016/j.str.2004.09.003 · PubMed

Other PDB entries of the same protein (UniProt O15085 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1HTJ 2.2 Å, Structure of the rgs-like domain from PDZ-rhogef
5JHH 2.3 Å, Crystal structure of the ternary complex between the human RhoA, its inhibitor and the…
5TYT 2.4 Å, Crystal Structure of the PDZ domain of RhoGEF bound to CXCR2 C-terminal peptide
5JHG 2.5 Å, Crystal structure of the complex between the human RhoA and the DH/PH domain of human…
3KZ1 2.7 Å, Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated…
3T06 2.84 Å, Crystal Structure of the DH/PH fragment of PDZRHOGEF with N-terminal regulatory elements…
5E6P 3.21 Å, PlexinB2 cytoplasmic region/PDZ-RhoGEF PDZ domain complex
2DLS Solution structure of the PDZ domain of human Rho guanine nucleotide exchange factor 11

Browse structure collections

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