29HG: CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase

Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase in complex with NSD2 via UNC10088. Determined by electron microscopy at 4.0 Å resolution. Released 6 May 2026.

Method
Electron microscopy
Resolution
4.0 Å
Organism
Homo sapiens
Chains
5
Atoms
12,381
Mol. weight
318.46 kDa
Ligands
A1J20
Released
6 May 2026

Explore 29HG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

29HG contains 71 α-helices and 49 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 17 β-strands

ElementResiduesLengthSheet
α-helix17-259
α-helix27-359
α-helix39-468
α-helix50-6213
β-strand67-7151
α-helix83-9412
α-helix98-992
β-strand101-10771
α-helix108-1114
α-helix130-1389
β-strand144-15071
β-strand152-15542
β-strand166-16832
β-strand174-17961
β-strand186-19493
α-helix204-2085
β-strand219-22573
α-helix232-24312
β-strand249-25463
β-strand257-25821
α-helix267-2682
β-strand273-28193
β-strand286-29162
α-helix299-31113
β-strand319-32572
α-helix340-3489
β-strand354-35852
β-strand36313
β-strand36912
α-helix383-3853
β-strand392-39872
Chain B: 9 helices, 7 β-strands
ElementResiduesLengthSheet
α-helix213-2175
β-strand225-22844
β-strand236-24054
α-helix255-2573
β-strand261-26664
α-helix2671
β-strand273-27754
α-helix278-2803
β-strand281-28334
α-helix287-2893
α-helix290-29910
α-helix318-32912
α-helix337-3448
β-strand347-34935
β-strand352-35435
α-helix357-3615
Chain C: 10 helices, 5 β-strands
ElementResiduesLengthSheet
β-strand3-866
β-strand13-1756
α-helix18-236
β-strand2417
α-helix25-339
β-strand45-4736
α-helix52-6514
α-helix68-703
α-helix71-733
α-helix87-926
α-helix97-11014
β-strand11217
α-helix113-12715
α-helix132-1398
α-helix147-15711
Chain D: 36 helices, 14 β-strands
ElementResiduesLengthSheet
α-helix17-3418
α-helix39-5416
α-helix86-10823
α-helix112-15140
α-helix159-1668
α-helix167-1726
α-helix173-19220
α-helix198-21114
α-helix226-2283
α-helix229-2335
α-helix234-25421
α-helix258-27821
α-helix281-29717
α-helix300-31112
α-helix316-32712
α-helix332-35928
α-helix363-38018
α-helix381-3855
α-helix389-40315
α-helix407-4126
α-helix417-43014
β-strand43118
α-helix439-45517
α-helix459-47618
β-strand47918
α-helix482-49615
α-helix498-52831
β-strand539-54139
α-helix557-57317
β-strand577-58159
α-helix583-5853
β-strand587-592610
β-strand600-604510
α-helix605-61612
β-strand621111
α-helix622-6298
α-helix633-64513
β-strand650112
α-helix652-6554
β-strand668111
α-helix6691
β-strand670112
α-helix6711
β-strand682-683210
α-helix689-72234
β-strand725-726213
α-helix727-73812
α-helix746-75813
β-strand763-764213
β-strand771-773313
Chain E: 2 helices, 6 β-strands
ElementResiduesLengthSheet
β-strand22-28710
β-strand31-3559
α-helix54-607
β-strand72114
β-strand73115
β-strand76115
α-helix81-877
β-strand107114

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
F-box only protein 22Aprotein403Homo sapiensQ8NEZ5 (AlphaFold model)
Histone-lysine N-methyltransferase NSD2Bprotein1365Homo sapiensO96028 (AlphaFold model)
S-phase kinase-associated protein 1Cprotein163Homo sapiensP63208 (AlphaFold model)
Cullin-1Dprotein776Homo sapiensQ13616 (AlphaFold model)
E3 ubiquitin-protein ligase RBX1Eprotein108Homo sapiensP62877
Sequence of entity 1 (A), FASTA
>29HG_1 F-box only protein 22 (chains A)
MEPVGCCGECRGSSVDPRSTFVLSNLAEVVERVLTFLPAKALLRVACVCRLWRECVRRVL
RTHRSVTWISAGLAEAGHLEGHCLVRVVAEELENVRILPHTVLYMADSETFISLEECRGH
KRARKRTSMETALALEKLFPKQCQVLGIVTPGIVVTPMGSGSNRPQEIEIGESGFALLFP
QIEGIKIQPFHFIKDPKNLTLERHQLTEVGLLDNPELRVVLVFGYNCCKVGASNYLQQVV
STFSDMNIILAGGQVDNLSSLTSEKNPLDIDASGVVGLSFSGHRIQSATVLLNEDVSDEK
TAEAAMQRLKAANIPEHNTIGFMFACVGRGFQYYRAKGNVEADAFRKFFPSVPLFGFFGN
GEIGCDRIVTGNFILRKCNEVKDDDLFHSYTTIMALIHLGSSK
Sequence of entity 2 (B), FASTA
>29HG_2 Histone-lysine N-methyltransferase NSD2 (chains B)
MEFSIKQSPLSVQSVVKCIKMKQAPEILGSANGKTPSCEVNRECSVFLSKAQLSSSLQEG
VMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNG
SPEIKLKITKTYMNGKPLFESSICGDSAADVSQSEENGQKPENKARRNRKRSIKYDSLLE
QGLVEAALVSKISSPSDKKIPAKKESCPNTGRDKDHLLKYNVGDLVWSKVSGYPWWPCMV
SADPLLHSYTKLKGQKKSARQYHVQFFGDAPERAWIFEKSLVAFEGEGQFEKLCQESAKQ
APTKAEKIKLLKPISGKLRAQWEMGIVQAEEAASMSVEERKAKFTFLYVGDQLHLNPQVA
KEAGIAAESLGEMAESSGVSEEAAENPKSVREECIPMKRRRRAKLCSSAETLESHPDIGK
STPQKTAEADPRRGVGSPPGRKKTTVSMPRSRKGDAASQFLVFCQKHRDEVVAEHPDASG
EEIEELLRSQWSLLSEKQRARYNTKFALVAPVQAEEDSGNVNGKKRNHTKRIQDPTEDAE
AEDTPRKRLRTDKHSLRKRDTITDKTARTSSYKAMEAASSLKSQAATKNLSDACKPLKKR
NRASTAASSALGFSKSSSPSASLTENEVSDSPGDEPSESPYESADETQTEVSVSSKKSER
GVTAKKEYVCQLCEKPGSLLLCEGPCCGAFHLACLGLSRRPEGRFTCSECASGIHSCFVC
KESKTDVKRCVVTQCGKFYHEACVKKYPLTVFESRGFRCPLHSCVSCHASNPSNPRPSKG
KMMRCVRCPVAYHSGDACLAAGCSVIASNSIICTAHFTARKGKRHHAHVNVSWCFVCSKG
GSLLCCESCPAAFHPDCLNIEMPDGSWFCNDCRAGKKLHFQDIIWVKLGNYRWWPAEVCH
PKNVPPNIQKMKHEIGEFPVFFFGSKDYYWTHQARVFPYMEGDRGSRYQGVRGIGRVFKN
ALQEAEARFREIKLQREARETQESERKPPPYKHIKVNKPYGKVQIYTADISEIPKCNCKP
TDENPCGFDSECLNRMLMFECHPQVCPAGEFCQNQCFTKRQYPETKIIKTDGKGWGLVAK
RDIRKGEFVNEYVGELIDEEECMARIKHAHENDITHFYMLTIDKDRIIDAGPKGNYSRFM
NHSCQPNCETLKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSG
FLGDRPKTSTTLSSEEKGKKTKKKTRRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCT
KAYHLSCLGLGKRPFGKWECPWHHCDVCGKPSTSFCHLCPNSFCKEHQDGTAFSCTPDGR
SYCCEHDLGAASVRSTKTEKPPPEPGKPKGKRRRRRGWRRVTEGK
Sequence of entity 3 (C), FASTA
>29HG_3 S-phase kinase-associated protein 1 (chains C)
MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ
WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTC
KTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK
Sequence of entity 4 (D), FASTA
>29HG_4 Cullin-1 (chains D)
MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSN
QARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYT
QQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVT
NAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADT
ERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHL
EIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAL
NDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPE
LLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSA
SDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVL
SSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYT
LQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDE
VELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMK
MRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA
Sequence of entity 5 (E), FASTA
>29HG_5 E3 ubiquitin-protein ligase RBX1 (chains E)
MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ
ASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH

Ligands and cofactors

IDNameFormulaCopies
A1J20~{N}-cyclopropyl-~{N}-[[4-[[2-(6-oxidanylhexanoyl)-1~{H}-isoquinolin-6-yl]carba…C35 H36 N4 O61

Primary citation

Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22. Robertson, K.C., Amann, S.J., Liu, T. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-72235-9 · PubMed

Other PDB entries of the same protein (UniProt Q8NEZ5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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