29HI: CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase

Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase in complex with NSD2 via UNC10415667. Determined by electron microscopy at 5.9 Å resolution. Released 6 May 2026.

Method
Electron microscopy
Resolution
5.9 Å
Organism
Homo sapiens
Chains
5
Atoms
12,383
Mol. weight
318.48 kDa
Ligands
A1J21
Released
6 May 2026

Explore 29HI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

29HI contains 70 α-helices and 42 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 17 β-strands

ElementResiduesLengthSheet
α-helix17-259
α-helix27-359
α-helix39-468
α-helix50-6213
β-strand67-7151
α-helix83-9412
α-helix98-992
β-strand101-10771
α-helix108-1114
α-helix130-1389
β-strand144-15071
β-strand152-15542
β-strand166-16832
β-strand174-17961
β-strand186-19493
α-helix203-2086
β-strand219-22573
α-helix232-24312
β-strand249-25463
β-strand257-25821
α-helix267-2682
β-strand273-28193
β-strand286-29162
α-helix299-31113
β-strand319-32572
α-helix340-3489
β-strand354-35852
β-strand36313
β-strand36912
α-helix383-3853
β-strand392-39872
Chain B: 9 helices, 7 β-strands
ElementResiduesLengthSheet
α-helix213-2175
β-strand225-22844
β-strand236-24054
α-helix255-2573
β-strand261-26664
α-helix2671
β-strand273-27754
α-helix278-2803
β-strand281-28334
α-helix287-2893
α-helix290-29910
α-helix318-32912
α-helix337-3448
β-strand347-34935
β-strand352-35435
α-helix357-3615
Chain C: 10 helices, 3 β-strands
ElementResiduesLengthSheet
β-strand3-866
β-strand13-1756
α-helix18-236
α-helix25-3410
α-helix43-442
β-strand45-4736
α-helix52-6413
α-helix71-744
α-helix87-926
α-helix97-11014
α-helix113-12715
α-helix132-1398
α-helix147-15711
Chain D: 34 helices, 13 β-strands
ElementResiduesLengthSheet
α-helix17-3418
α-helix39-5416
α-helix86-10823
α-helix112-15039
α-helix159-1668
α-helix167-1726
α-helix173-19220
α-helix198-21013
α-helix226-25429
α-helix257-27822
α-helix281-31333
α-helix316-32712
α-helix330-3323
α-helix333-36129
α-helix363-38119
α-helix382-3865
α-helix389-40315
α-helix407-4126
α-helix417-43014
β-strand43117
α-helix432-4332
α-helix440-45516
α-helix459-47618
β-strand47917
α-helix482-49615
α-helix498-52831
β-strand537-54158
α-helix542-5443
α-helix557-57317
β-strand577-58158
α-helix583-5853
β-strand587-59159
β-strand600-60459
α-helix605-61612
β-strand619-621310
α-helix622-6298
α-helix633-64513
β-strand650110
α-helix653-6553
β-strand668-670310
β-strand681-68339
α-helix689-72133
β-strand724-726311
α-helix727-73812
α-helix746-75813
β-strand762-765411
β-strand771-774411
Chain E: 3 helices, 2 β-strands
ElementResiduesLengthSheet
β-strand26-2839
β-strand29-3578
α-helix43-453
α-helix54-607
α-helix81-888

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
F-box only protein 22Aprotein403Homo sapiensQ8NEZ5 (AlphaFold model)
Histone-lysine N-methyltransferase NSD2Bprotein1365Homo sapiensO96028 (AlphaFold model)
S-phase kinase-associated protein 1Cprotein163Homo sapiensP63208 (AlphaFold model)
Cullin-1Dprotein776Homo sapiensQ13616 (AlphaFold model)
E3 ubiquitin-protein ligase RBX1Eprotein108Homo sapiensP62877
Sequence of entity 1 (A), FASTA
>29HI_1 F-box only protein 22 (chains A)
MEPVGCCGECRGSSVDPRSTFVLSNLAEVVERVLTFLPAKALLRVACVCRLWRECVRRVL
RTHRSVTWISAGLAEAGHLEGHCLVRVVAEELENVRILPHTVLYMADSETFISLEECRGH
KRARKRTSMETALALEKLFPKQCQVLGIVTPGIVVTPMGSGSNRPQEIEIGESGFALLFP
QIEGIKIQPFHFIKDPKNLTLERHQLTEVGLLDNPELRVVLVFGYNCCKVGASNYLQQVV
STFSDMNIILAGGQVDNLSSLTSEKNPLDIDASGVVGLSFSGHRIQSATVLLNEDVSDEK
TAEAAMQRLKAANIPEHNTIGFMFACVGRGFQYYRAKGNVEADAFRKFFPSVPLFGFFGN
GEIGCDRIVTGNFILRKCNEVKDDDLFHSYTTIMALIHLGSSK
Sequence of entity 2 (B), FASTA
>29HI_2 Histone-lysine N-methyltransferase NSD2 (chains B)
MEFSIKQSPLSVQSVVKCIKMKQAPEILGSANGKTPSCEVNRECSVFLSKAQLSSSLQEG
VMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNG
SPEIKLKITKTYMNGKPLFESSICGDSAADVSQSEENGQKPENKARRNRKRSIKYDSLLE
QGLVEAALVSKISSPSDKKIPAKKESCPNTGRDKDHLLKYNVGDLVWSKVSGYPWWPCMV
SADPLLHSYTKLKGQKKSARQYHVQFFGDAPERAWIFEKSLVAFEGEGQFEKLCQESAKQ
APTKAEKIKLLKPISGKLRAQWEMGIVQAEEAASMSVEERKAKFTFLYVGDQLHLNPQVA
KEAGIAAESLGEMAESSGVSEEAAENPKSVREECIPMKRRRRAKLCSSAETLESHPDIGK
STPQKTAEADPRRGVGSPPGRKKTTVSMPRSRKGDAASQFLVFCQKHRDEVVAEHPDASG
EEIEELLRSQWSLLSEKQRARYNTKFALVAPVQAEEDSGNVNGKKRNHTKRIQDPTEDAE
AEDTPRKRLRTDKHSLRKRDTITDKTARTSSYKAMEAASSLKSQAATKNLSDACKPLKKR
NRASTAASSALGFSKSSSPSASLTENEVSDSPGDEPSESPYESADETQTEVSVSSKKSER
GVTAKKEYVCQLCEKPGSLLLCEGPCCGAFHLACLGLSRRPEGRFTCSECASGIHSCFVC
KESKTDVKRCVVTQCGKFYHEACVKKYPLTVFESRGFRCPLHSCVSCHASNPSNPRPSKG
KMMRCVRCPVAYHSGDACLAAGCSVIASNSIICTAHFTARKGKRHHAHVNVSWCFVCSKG
GSLLCCESCPAAFHPDCLNIEMPDGSWFCNDCRAGKKLHFQDIIWVKLGNYRWWPAEVCH
PKNVPPNIQKMKHEIGEFPVFFFGSKDYYWTHQARVFPYMEGDRGSRYQGVRGIGRVFKN
ALQEAEARFREIKLQREARETQESERKPPPYKHIKVNKPYGKVQIYTADISEIPKCNCKP
TDENPCGFDSECLNRMLMFECHPQVCPAGEFCQNQCFTKRQYPETKIIKTDGKGWGLVAK
RDIRKGEFVNEYVGELIDEEECMARIKHAHENDITHFYMLTIDKDRIIDAGPKGNYSRFM
NHSCQPNCETLKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSG
FLGDRPKTSTTLSSEEKGKKTKKKTRRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCT
KAYHLSCLGLGKRPFGKWECPWHHCDVCGKPSTSFCHLCPNSFCKEHQDGTAFSCTPDGR
SYCCEHDLGAASVRSTKTEKPPPEPGKPKGKRRRRRGWRRVTEGK
Sequence of entity 3 (C), FASTA
>29HI_3 S-phase kinase-associated protein 1 (chains C)
MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ
WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTC
KTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK
Sequence of entity 4 (D), FASTA
>29HI_4 Cullin-1 (chains D)
MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSN
QARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYT
QQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVT
NAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADT
ERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHL
EIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAL
NDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPE
LLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSA
SDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVL
SSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYT
LQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDE
VELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMK
MRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA
Sequence of entity 5 (E), FASTA
>29HI_5 E3 ubiquitin-protein ligase RBX1 (chains E)
MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ
ASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH

Ligands and cofactors

IDNameFormulaCopies
A1J214-[[cyclopropyl-[(~{Z})-2-methyl-4-(5-oxidanylidene-2~{H}-1,4-oxazin-3-yl)but-3…C37 H40 N4 O61

Primary citation

Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22. Robertson, K.C., Amann, S.J., Liu, T. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-72235-9 · PubMed

Other PDB entries of the same protein (UniProt Q8NEZ5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 29HI directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.