29HH: CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase

Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase in complex with NSD2, UNC10088 and Bach1. Determined by electron microscopy at 4.2 Å resolution. Released 6 May 2026.

Method
Electron microscopy
Resolution
4.2 Å
Organism
Homo sapiens
Chains
7
Atoms
14,314
Mol. weight
482.56 kDa
Ligands
A1J20
Released
6 May 2026

Explore 29HH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

29HH contains 80 α-helices and 49 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 18 β-strands

ElementResiduesLengthSheet
α-helix17-259
α-helix27-359
α-helix39-468
α-helix53-6210
β-strand67-7151
α-helix83-9412
α-helix98-992
β-strand101-10771
α-helix108-1114
α-helix130-1378
β-strand144-15071
β-strand152-15542
β-strand166-16832
β-strand174-17961
β-strand186-19493
α-helix203-2086
β-strand219-22573
α-helix232-24312
β-strand249-25463
β-strand257-25821
α-helix267-2682
β-strand273-28193
β-strand286-29162
α-helix292-2932
α-helix301-31212
β-strand319-32572
α-helix340-3489
β-strand354-35852
β-strand363-36423
β-strand36912
β-strand374-37524
β-strand392-39872
Chain B: 8 helices, 7 β-strands
ElementResiduesLengthSheet
α-helix213-2175
β-strand225-22845
β-strand236-24055
α-helix255-2573
β-strand261-26665
β-strand273-27755
α-helix278-2803
β-strand281-28335
α-helix287-2893
α-helix290-29910
α-helix318-32912
α-helix337-3448
β-strand347-34936
β-strand352-35436
α-helix357-3615
Chain C: 11 helices, 3 β-strands
ElementResiduesLengthSheet
β-strand3-867
β-strand13-1757
α-helix18-236
α-helix25-328
α-helix441
β-strand45-4737
α-helix52-6413
α-helix68-703
α-helix71-733
α-helix87-926
α-helix97-11014
α-helix113-12715
α-helix132-1398
α-helix147-15711
Chain D: 32 helices, 11 β-strands
ElementResiduesLengthSheet
α-helix17-3418
α-helix39-5416
α-helix86-10823
α-helix112-15039
α-helix159-1668
α-helix167-1726
α-helix173-19220
α-helix199-21113
α-helix225-2284
α-helix229-2335
α-helix234-25421
α-helix257-27822
α-helix282-31130
α-helix316-32611
α-helix333-35624
α-helix363-38422
α-helix389-40416
α-helix408-4125
α-helix417-43014
β-strand43118
α-helix436-4383
α-helix439-45315
α-helix459-47517
β-strand47918
α-helix482-49615
α-helix498-52730
β-strand537-54159
α-helix557-57317
β-strand577-58159
β-strand587-592610
β-strand600-604510
α-helix605-61511
β-strand619-621311
α-helix622-6298
α-helix633-64513
α-helix652-6565
β-strand668-670311
α-helix689-72234
β-strand724-725212
α-helix727-73812
α-helix746-75813
β-strand764-765212
β-strand771-773312
Chain E: 2 helices, 2 β-strands
ElementResiduesLengthSheet
β-strand22-28710
β-strand29-3579
α-helix54-607
α-helix82-887
Chain G: 7 helices, 4 β-strands
ElementResiduesLengthSheet
α-helix14-2916
β-strand37-40413
β-strand43-45313
α-helix49-568
α-helix70-712
β-strand72-74313
α-helix75-762
α-helix80-9314
β-strand95-9844
α-helix100-11314
α-helix120-1256
Chain H: 6 helices, 4 β-strands
ElementResiduesLengthSheet
β-strand9-1244
α-helix16-2813
β-strand36-40514
β-strand43-47514
α-helix49-557
α-helix57-637
β-strand72-73214
α-helix81-9212
α-helix100-11213
α-helix120-1234

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
F-box only protein 22Aprotein403Homo sapiensQ8NEZ5 (AlphaFold model)
Histone-lysine N-methyltransferase NSD2Bprotein1365Homo sapiensO96028 (AlphaFold model)
S-phase kinase-associated protein 1Cprotein163Homo sapiensP63208 (AlphaFold model)
Cullin-1Dprotein776Homo sapiensQ13616 (AlphaFold model)
E3 ubiquitin-protein ligase RBX1Eprotein108Homo sapiensP62877
Transcription regulator protein BACH1G, Hprotein736Homo sapiensO14867
Sequence of entity 1 (A), FASTA
>29HH_1 F-box only protein 22 (chains A)
MEPVGCCGECRGSSVDPRSTFVLSNLAEVVERVLTFLPAKALLRVACVCRLWRECVRRVL
RTHRSVTWISAGLAEAGHLEGHCLVRVVAEELENVRILPHTVLYMADSETFISLEECRGH
KRARKRTSMETALALEKLFPKQCQVLGIVTPGIVVTPMGSGSNRPQEIEIGESGFALLFP
QIEGIKIQPFHFIKDPKNLTLERHQLTEVGLLDNPELRVVLVFGYNCCKVGASNYLQQVV
STFSDMNIILAGGQVDNLSSLTSEKNPLDIDASGVVGLSFSGHRIQSATVLLNEDVSDEK
TAEAAMQRLKAANIPEHNTIGFMFACVGRGFQYYRAKGNVEADAFRKFFPSVPLFGFFGN
GEIGCDRIVTGNFILRKCNEVKDDDLFHSYTTIMALIHLGSSK
Sequence of entity 2 (B), FASTA
>29HH_2 Histone-lysine N-methyltransferase NSD2 (chains B)
MEFSIKQSPLSVQSVVKCIKMKQAPEILGSANGKTPSCEVNRECSVFLSKAQLSSSLQEG
VMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNG
SPEIKLKITKTYMNGKPLFESSICGDSAADVSQSEENGQKPENKARRNRKRSIKYDSLLE
QGLVEAALVSKISSPSDKKIPAKKESCPNTGRDKDHLLKYNVGDLVWSKVSGYPWWPCMV
SADPLLHSYTKLKGQKKSARQYHVQFFGDAPERAWIFEKSLVAFEGEGQFEKLCQESAKQ
APTKAEKIKLLKPISGKLRAQWEMGIVQAEEAASMSVEERKAKFTFLYVGDQLHLNPQVA
KEAGIAAESLGEMAESSGVSEEAAENPKSVREECIPMKRRRRAKLCSSAETLESHPDIGK
STPQKTAEADPRRGVGSPPGRKKTTVSMPRSRKGDAASQFLVFCQKHRDEVVAEHPDASG
EEIEELLRSQWSLLSEKQRARYNTKFALVAPVQAEEDSGNVNGKKRNHTKRIQDPTEDAE
AEDTPRKRLRTDKHSLRKRDTITDKTARTSSYKAMEAASSLKSQAATKNLSDACKPLKKR
NRASTAASSALGFSKSSSPSASLTENEVSDSPGDEPSESPYESADETQTEVSVSSKKSER
GVTAKKEYVCQLCEKPGSLLLCEGPCCGAFHLACLGLSRRPEGRFTCSECASGIHSCFVC
KESKTDVKRCVVTQCGKFYHEACVKKYPLTVFESRGFRCPLHSCVSCHASNPSNPRPSKG
KMMRCVRCPVAYHSGDACLAAGCSVIASNSIICTAHFTARKGKRHHAHVNVSWCFVCSKG
GSLLCCESCPAAFHPDCLNIEMPDGSWFCNDCRAGKKLHFQDIIWVKLGNYRWWPAEVCH
PKNVPPNIQKMKHEIGEFPVFFFGSKDYYWTHQARVFPYMEGDRGSRYQGVRGIGRVFKN
ALQEAEARFREIKLQREARETQESERKPPPYKHIKVNKPYGKVQIYTADISEIPKCNCKP
TDENPCGFDSECLNRMLMFECHPQVCPAGEFCQNQCFTKRQYPETKIIKTDGKGWGLVAK
RDIRKGEFVNEYVGELIDEEECMARIKHAHENDITHFYMLTIDKDRIIDAGPKGNYSRFM
NHSCQPNCETLKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSG
FLGDRPKTSTTLSSEEKGKKTKKKTRRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCT
KAYHLSCLGLGKRPFGKWECPWHHCDVCGKPSTSFCHLCPNSFCKEHQDGTAFSCTPDGR
SYCCEHDLGAASVRSTKTEKPPPEPGKPKGKRRRRRGWRRVTEGK
Sequence of entity 3 (C), FASTA
>29HH_3 S-phase kinase-associated protein 1 (chains C)
MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ
WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTC
KTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK
Sequence of entity 4 (D), FASTA
>29HH_4 Cullin-1 (chains D)
MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSN
QARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYT
QQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVT
NAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADT
ERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHL
EIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAL
NDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPE
LLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSA
SDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVL
SSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYT
LQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDE
VELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMK
MRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA
Sequence of entity 5 (E), FASTA
>29HH_5 E3 ubiquitin-protein ligase RBX1 (chains E)
MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ
ASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH
Sequence of entity 6 (G, H), FASTA
>29HH_6 Transcription regulator protein BACH1 (chains G, H)
MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRSVLAACSSYFH
SRIVGQADGELNITLPEEVTVKGFEPLIQFAYTAKLILSKENVDEVCKCVEFLSVHNIEE
SCFQFLKFKFLDSTADQQECPRKKCFSSHCQKTDLKLSLLDQRDLETDEVEEFLENKNVQ
TPQCKLRRYQGNAKASPPLQDSASQTYESMCLEKDAALALPSLCPKYRKFQKAFGTDRVR
TGESSVKDIHASVQPNERSENECLGGVPECRDLQVMLKCDESKLAMEPEETKKDPASQCP
TEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKPLSGTDVQEKT
FGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAV
AKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGN
DDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRND
FQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLL
KERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSFLISEKDKSTP
DGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQMSTATSEQAGPAEQCRQSGG
ISDFCQQMTDKCTTDE

Ligands and cofactors

IDNameFormulaCopies
A1J20~{N}-cyclopropyl-~{N}-[[4-[[2-(6-oxidanylhexanoyl)-1~{H}-isoquinolin-6-yl]carba…C35 H36 N4 O61

Primary citation

Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22. Robertson, K.C., Amann, S.J., Liu, T. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-72235-9 · PubMed

Other PDB entries of the same protein (UniProt Q8NEZ5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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