8UA6: SCF-FBOX22-BACH1BTB

Cryo-EM Structure of SCF-FBOX22-BACH1BTB. Determined by electron microscopy at 3.9 Å resolution. Released 6 Nov 2024.

Method: Electron microscopy
Resolution: 3.9 Å
Organism: Homo sapiens
Chains: 5
Atoms: 9,806
Mol. weight: 178.83 kDa
Released: 6 Nov 2024

Explore 8UA6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8UA6 contains 65 α-helices and 45 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 30 helices, 0 β-strands

Element	Residues	Length
α-helix	20-22	3
α-helix	24-26	3
α-helix	39-53	15
α-helix	87-94	8
α-helix	96-99	4
α-helix	124-127	4
α-helix	129-131	3
α-helix	139-142	4
α-helix	159-166	8
α-helix	180-187	8
α-helix	198-210	13
α-helix	226-228	3
α-helix	229-233	5
α-helix	234-241	8
α-helix	248-251	4
α-helix	257-270	14
α-helix	284-287	4
α-helix	289-291	3
α-helix	304-312	9
α-helix	318-328	11
α-helix	338-354	17
α-helix	358-361	4
α-helix	363-379	17
α-helix	380-384	5
α-helix	389-395	7
α-helix	400-402	3
α-helix	417-429	13
α-helix	439-453	15
α-helix	460-476	17
α-helix	482-494	13

Chain B: 9 helices, 3 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	14
β-strand	13-16	4	14
α-helix	18-21	4
α-helix	25-32	8
β-strand	45-46	2	14
α-helix	52-64	13
α-helix	87-93	7
α-helix	97-110	14
α-helix	113-127	15
α-helix	133-135	3
α-helix	136-139	4
α-helix	147-153	7

Chain C: 15 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	22-25	4
α-helix	27-34	8
α-helix	39-42	4
α-helix	45-47	3
α-helix	50-59	10
β-strand	67-71	5	1
α-helix	73-75	3
α-helix	83-91	9
α-helix	92-94	3
β-strand	101-106	6	1
α-helix	128-138	11
β-strand	144-149	6	1
β-strand	153-155	3	2
α-helix	163-165	3
β-strand	166-168	3	2
β-strand	174-179	6	1
β-strand	190-193	4	3
α-helix	205-208	4
β-strand	217-220	4	4
β-strand	223-225	3	3
α-helix	232-243	12
β-strand	249	1	4
β-strand	252	1	5
β-strand	254-255	2	3
β-strand	257-261	5	1
α-helix	267-269	3
β-strand	274-277	4	3
β-strand	279-281	3	4
β-strand	286	1	6
β-strand	289	1	7
β-strand	291-292	2	2
α-helix	299-310	12
β-strand	320-324	5	2
β-strand	325-326	2	8
α-helix	340-348	9
β-strand	354-357	4	2
β-strand	358-359	2	8
β-strand	362-363	2	5
β-strand	367	1	7
β-strand	369	1	9
β-strand	373-374	2	9
β-strand	377	1	10
β-strand	387-388	2	5
β-strand	392-397	6	2
β-strand	398	1	6

Chain D: 5 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	12	1	11
α-helix	16-29	14
β-strand	36-40	5	12
β-strand	45-47	3	12
α-helix	49-55	7
α-helix	70-71	2
β-strand	72-75	4	12
α-helix	81-93	13
β-strand	95	1	9
β-strand	98	1	10
α-helix	102-111	10

Chain E: 6 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	9	1	10
β-strand	12-13	2	9
α-helix	16-28	13
β-strand	36-40	5	13
β-strand	43-47	5	13
α-helix	52-55	4
α-helix	58-63	6
α-helix	67	1
β-strand	72	1	13
α-helix	85-93	9
β-strand	95	1	11
α-helix	103-113	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
F-box only protein 22	C	protein	403	Homo sapiens	Q8NEZ5 (AlphaFold model)
Transcription regulator protein BACH1	D, E	protein	122	Homo sapiens	O14867 (AlphaFold model)
S-phase kinase-associated protein 1	B	protein	160	Homo sapiens	P63208 (AlphaFold model)
Cullin-1	A	protein	764	Homo sapiens	Q13616 (AlphaFold model)

Sequence of entity 1 (C), FASTA

>8UA6_1 F-box only protein 22 (chains C)
MEPVGCCGECRGSSVDPRSTFVLSNLAEVVERVLTFLPAKALLRVACVCRLWRECVRRVL
RTHRSVTWISAGLAEAGHLEGHCLVRVVAEELENVRILPHTVLYMADSETFISLEECRGH
KRARKRTSMETALALEKLFPKQCQVLGIVTPGIVVTPMGSGSNRPQEIEIGESGFALLFP
QIEGIKIQPFHFIKDPKNLTLERHQLTEVGLLDNPELRVVLVFGYNCCKVGASNYLQQVV
STFSDMNIILAGGQVDNLSSLTSEKNPLDIDASGVVGLSFSGHRIQSATVLLNEDVSDEK
TAEAAMQRLKAANIPEHNTIGFMFACVGRGFQYYRAKGNVEADAFRKFFPSVPLFGFFGN
GEIGCDRIVTGNFILRKCNEVKDDDLFHSYTTIMALIHLGSSK

Sequence of entity 2 (D, E), FASTA

>8UA6_2 Transcription regulator protein BACH1 (chains D, E)
SVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRSVLAACSSYFHSRIVGQ
ADGELNITLPEEVTVKGFEPLIQFAYTAKLILSKENVDEVCKCVEFLSVHNIEESCFQFL
KF

Sequence of entity 3 (B), FASTA

>8UA6_3 S-phase kinase-associated protein 1 (chains B)
MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ
WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTC
KTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVGSTQFCL

Sequence of entity 4 (A), FASTA

>8UA6_4 Cullin-1 (chains A)
KQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKS
KKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKV
LNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERN
GETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFL
QQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLD
ADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLD
VHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKK
SSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKL
KQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCT
FALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILL
QYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLY
LGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLG
EVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Primary citation

Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress. Cao, S., Garcia, S.F., Shi, H. et al. Cell (2024) 187:7568-7584.e22. DOI 10.1016/j.cell.2024.10.012 · PubMed

Other PDB entries of the same protein (UniProt Q8NEZ5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8S7D 3.2 Å, Cryo-EM structure of SKP1-FBXO22 in complex with a BACH1 BTB dimer at 3.2A resolution
8S7E 3.4 Å, Cryo-EM structure of SKP1-FBXO22
8UA3 3.8 Å, Cryo-EM Structure of FBOX22-BACH1BTB
29HG 4.0 Å, Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase in complex with…
29HH 4.2 Å, Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase in complex with…
29HI 5.9 Å, Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO22 SCF ubiquition ligase in complex with…

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