2DFS: 3-D structure of Myosin-V inhibited state

3-D structure of Myosin-V inhibited state. Determined by electron crystallography at 24.0 Å resolution. Released 25 Apr 2006.

Method: Electron crystallography
Resolution: 24.0 Å
Organisms: Gallus gallus, Mus musculus
Chains: 14
Atoms: 29,614
Mol. weight: 453.49 kDa
Released: 25 Apr 2006

Explore 2DFS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2DFS contains 193 α-helices and 110 β-strands across 14 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 39 helices, 31 β-strands

Element	Residues	Length	Sheet
β-strand	12-17	6	1
β-strand	21-26	6	1
β-strand	37-39	3	2
β-strand	49-51	3	2
α-helix	60-61	2
β-strand	62	1	1
α-helix	63-65	3
α-helix	66-68	3
β-strand	73	1	3
α-helix	82-94	13
β-strand	100-103	4	3
β-strand	105-110	6	3
α-helix	121-127	7
α-helix	132-134	3
α-helix	139-153	15
β-strand	157-162	6	3
α-helix	169-182	14
α-helix	193-207	15
β-strand	208-211	4	4
β-strand	214-218	5	4
β-strand	221-228	8	3
β-strand	234-242	9	3
α-helix	247-249	3
β-strand	259	1	4
α-helix	260-267	8
α-helix	273-276	4
α-helix	301-314	14
α-helix	319-335	17
β-strand	341-343	3	5
β-strand	347-349	3	5
α-helix	350-351	2
α-helix	355-364	10
α-helix	368-376	9
β-strand	377-380	4	6
β-strand	387-390	4	6
α-helix	393-423	31
α-helix	428-429	2
β-strand	431-438	8	3
β-strand	447	1	7
α-helix	449-480	32
α-helix	493-500	8
α-helix	505-512	8
α-helix	520-531	12
β-strand	532	1	8
β-strand	535	1	8
β-strand	538-539	2	7
β-strand	547-551	5	7
β-strand	556-560	5	7
α-helix	564-568	5
β-strand	569	1	9
α-helix	574-581	8
α-helix	588-591	4
β-strand	635	1	9
α-helix	636-652	17
β-strand	655-662	8	3
α-helix	675-683	9
α-helix	687-694	8
β-strand	700-703	4	10
α-helix	704-711	8
α-helix	717-719	3
α-helix	724-732	9
α-helix	739-741	3
β-strand	742-744	3	10
β-strand	748-751	4	10
α-helix	755-874	120
α-helix	878-879	2
α-helix	880-887	8
α-helix	888-905	18
α-helix	952-967	16
α-helix	969-1078	110

Chain B: 10 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	13-16	4
β-strand	26-28	3	11
α-helix	29-31	3
α-helix	32-39	8
α-helix	45-53	9
β-strand	62-64	3	11
α-helix	65-74	10
α-helix	76-80	5
α-helix	81-83	3
β-strand	99-101	3	12
α-helix	102-110	9
α-helix	118-124	7
β-strand	135-137	3	12
α-helix	138-146	9

Chains C and O: 10 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	9-17	9
β-strand	26-28	3	13
α-helix	29-31	3
α-helix	32-39	8
α-helix	45-52	8
α-helix	56-59	4
β-strand	62-64	3	13
α-helix	65-81	17
β-strand	99-101	3	14
α-helix	102-111	10
α-helix	115-117	3
α-helix	118-124	7
β-strand	135-137	3	14
α-helix	138-146	9

Chains D and P: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	13-19	7
β-strand	26-28	3	15
α-helix	29-31	3
α-helix	32-39	8
α-helix	45-53	9
β-strand	62-64	3	15
α-helix	65-74	10
α-helix	76-81	6
β-strand	99-101	3	16
α-helix	102-110	9
α-helix	118-124	7
β-strand	135-137	3	16
α-helix	138-146	9

Chain E: 10 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	9-19	11
β-strand	26-28	3	17
α-helix	29-31	3
α-helix	32-39	8
α-helix	45-52	8
α-helix	56-59	4
β-strand	62-64	3	17
α-helix	65-81	17
β-strand	99-101	3	18
α-helix	102-111	10
α-helix	115-117	3
α-helix	118-124	7
β-strand	135-137	3	18
α-helix	138-146	9

Chain F: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	13-19	7
β-strand	26-28	3	19
α-helix	29-31	3
α-helix	32-39	8
α-helix	45-53	9
β-strand	62-64	3	19
α-helix	65-74	10
α-helix	76-81	6
β-strand	99-101	3	20
α-helix	102-111	10
α-helix	118-125	8
β-strand	135-137	3	20
α-helix	138-146	9

Chain G: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	9-19	11
β-strand	26-28	3	21
α-helix	29-31	3
α-helix	32-39	8
α-helix	45-51	7
β-strand	62-64	3	21
α-helix	65-75	11
α-helix	77-81	5
β-strand	99-101	3	22
α-helix	102-111	10
α-helix	118-125	8
β-strand	135-137	3	22
α-helix	138-146	9

Chain M: 41 helices, 31 β-strands

Element	Residues	Length	Sheet
β-strand	12-17	6	23
β-strand	21-26	6	23
β-strand	37-39	3	24
β-strand	49-51	3	24
α-helix	52-53	2
α-helix	60-61	2
β-strand	62	1	23
α-helix	63-65	3
α-helix	66-68	3
β-strand	73	1	25
α-helix	82-94	13
β-strand	100-103	4	25
β-strand	106-110	5	25
α-helix	121-127	7
α-helix	132-134	3
α-helix	139-153	15
β-strand	157-162	6	25
α-helix	169-183	15
α-helix	193-207	15
β-strand	208-211	4	26
β-strand	214-218	5	26
β-strand	221-228	8	25
β-strand	234-242	9	25
α-helix	247-249	3
β-strand	259	1	26
α-helix	260-267	8
α-helix	273-276	4
α-helix	301-314	14
α-helix	319-335	17
β-strand	341-343	3	27
β-strand	347-349	3	27
α-helix	350-351	2
α-helix	355-364	10
α-helix	368-376	9
β-strand	377-380	4	28
β-strand	387-390	4	28
α-helix	393-421	29
α-helix	428-429	2
β-strand	431-438	8	25
β-strand	447	1	29
α-helix	449-480	32
α-helix	493-500	8
α-helix	505-512	8
α-helix	520-531	12
β-strand	532	1	30
β-strand	535	1	30
β-strand	538-539	2	29
β-strand	547-551	5	29
β-strand	556-560	5	29
α-helix	564-568	5
β-strand	569	1	31
α-helix	574-581	8
α-helix	588-591	4
β-strand	635	1	31
α-helix	636-652	17
β-strand	655-662	8	25
α-helix	675-683	9
α-helix	687-694	8
β-strand	700-703	4	32
α-helix	704-711	8
α-helix	717-719	3
α-helix	724-732	9
α-helix	739-741	3
β-strand	742-744	3	32
β-strand	748-751	4	32
α-helix	755-874	120
α-helix	875-877	3
α-helix	878-879	2
α-helix	880-887	8
α-helix	888-905	18
α-helix	952-967	16
α-helix	969-1079	111

4 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Myosin-5A	A, M	protein	1080	Gallus gallus	Q02440 (AlphaFold model)
Calmodulin	B, C, D, E, F, G, N, O, P, Q, R, S	protein	148	Mus musculus	P0DP26 (AlphaFold model)

Sequence of entity 1 (A, M), FASTA

>2DFS_1 Myosin-5A (chains A, M)
MAASELYTKYARVWIPDPEEVWKSAELLKDYKPGDKVLQLRLEEGKDLEYCLDPKTKELP
PLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYG
EDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYF
ATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMR
TYLLEKSRVVFQAEEERNYHIFYQLCASAALPEFKTLRLGNANYFHYTKQGGSPVIDGID
DAKEMVNTRQACTLLGISDSYQMGIFRILAGILHLGNVEFASRDSDSCAIPPKHDPLTIF
CDLMGVDYEEMAHWLCHRKLATATETYIKPISKLHAINARDALAKHIYANLFNWIVDHVN
KALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKE
QIPWTLIDFYDNQPCINLIEAKMGVLDLLDEECKMPKGSDDTWAQKLYNTHLNKCALFEK
PRLSNKAFIIKHFADKVEYQCEGFLEKNKDTVYEEQIKVLKSSKKFKLLPELFQDEEKAI
SPTSATPSGRVPLSRTPVKPAKARPGQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVR
CIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDV
LSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRAACIRIQKTIRG
WLMRKKYMRMRRAAITIQRYVRGHQARCYATFLRRTRAAIIIQKFQRMYVVRKRYQCMRD
ATIALQALLRGYLVRNKYQMMLREHKSIIIQKHVRGWLARVHYHRTLKAIVYLQCCYRRM
MAKRELKKLKIEARSVERYKKLHIGLENKIMQLQRKIDEQNKEYKSLLEKMNNLEITYST
ETEKLRSDVERLRMSEEEAKNATNRVLSLQEEIAKLRKELHQTQTEKKTIEEWADKYKHE
TEQLVSELKEQNTLLKTEKEELNRRIHDQAKEITETMEKKLVEETKQLELDLNDERLRYQ

Sequence of entity 2 (B, C, D, E, F, G, N, O, P, Q, R, S), FASTA

>2DFS_2 Calmodulin (chains B, C, D, E, F, G, N, O, P, Q, R, S)
ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEEFVQMMTAK

Primary citation

Three-dimensional structure of the myosin V inhibited state by cryoelectron tomography. Liu, J., Taylor, D.W., Krementsova, E.B. et al. Nature (2006) 442:208-211. DOI 10.1038/nature04719 · PubMed

Other PDB entries of the same protein (UniProt Q02440 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1W7J 2.0 Å, Crystal Structure Of Myosin V Motor With Essential Light Chain + ADP-BeFx - Near Rigor
1OE9 2.05 Å, Crystal structure of Myosin V motor with essential light chain-nucleotide-free
1W8J 2.7 Å, Crystal Structure Of Myosin V Motor Domain - Nucleotide-Free
7PMD 2.9 Å, Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state…
7PME 2.9 Å, Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state…
1W7I 3.0 Å, Crystal Structure Of Myosin V Motor Without nucleotide soaked in 10 mM MgADP
7PM6 3.0 Å, Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 3er/2er)
7PM5 3.1 Å, Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er)
7PLU 3.2 Å, Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er)
7PLY 3.2 Å, Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young…
7PLZ 3.2 Å, Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er, young…
7PLT 3.3 Å, Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er)

Browse structure collections

About this viewer

MolViewer shows 2DFS directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.