2FF3: Gelsolin domain 1:N-wasp V2 motif hybrid

Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin. Determined by X-ray diffraction at 2.0 Å resolution. Released 21 Mar 2006.

Method: X-ray diffraction
Resolution: 2.0 Å
Organisms: Homo sapiens, Oryctolagus cuniculus
Chains: 3
Atoms: 4,192
Mol. weight: 60.81 kDa
Ligands: CA, ATP
Released: 21 Mar 2006

Explore 2FF3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2FF3 contains 33 α-helices and 28 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	30-34	5
β-strand	40-47	8	1
β-strand	50-53	4	1
α-helix	54-55	2
α-helix	56-58	3
β-strand	61-63	3	2
β-strand	67-75	9	1
β-strand	81-89	9	1
α-helix	95-111	17
α-helix	116	1
β-strand	117-122	6	1
α-helix	128-132	5
β-strand	139-141	3	2
α-helix	145-147	3
α-helix	149	1
β-strand	150	1	3
α-helix	151	1

Chain B: 24 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	8-12	5	4
β-strand	16-21	6	4
β-strand	24	1	3
β-strand	29-32	4	4
β-strand	35-37	3	5
β-strand	53-54	2	5
α-helix	56-60	5
α-helix	62-64	3
β-strand	66-68	3	5
β-strand	71-72	2	6
β-strand	75-76	2	6
α-helix	79-88	10
α-helix	89-94	6
α-helix	98-100	3
β-strand	103-107	5	4
α-helix	113-125	13
β-strand	131-136	6	4
α-helix	137-144	8
β-strand	150-155	6	7
β-strand	160-166	7	7
β-strand	169-170	2	7
α-helix	172-174	3
β-strand	176-178	3	7
α-helix	182-196	15
α-helix	203-216	14
α-helix	223-232	10
β-strand	238-241	4	8
β-strand	247-250	4	8
α-helix	253-259	7
α-helix	264-267	4
α-helix	272-273	2
α-helix	274-283	10
α-helix	287-289	3
α-helix	290-294	5
β-strand	297-300	4	7
α-helix	302-304	3
α-helix	309-320	12
β-strand	329-330	2	7
α-helix	338-348	11
α-helix	350-352	3
β-strand	357-358	2	4
α-helix	359-365	7
α-helix	367-369	3
α-helix	370-373	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Gelsolin	A	protein	150	Homo sapiens	P06396 (AlphaFold model)
Neural Wiskott-Aldrich syndrome protein	C	protein	15	Homo sapiens	O00401 (AlphaFold model)
Actin, alpha skeletal muscle	B	protein	375	Oryctolagus cuniculus	P68135 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2FF3_1 Gelsolin (chains A)
MKHHHHHHHHGGLVPRGSHGGSMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDF
FTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREV
QGFESATFLGYFKSGLKYKKGGVASGFKHV

Sequence of entity 2 (C), FASTA

>2FF3_2 Neural Wiskott-Aldrich syndrome protein (chains C)
ADGQESTPPTPAPTS

Sequence of entity 3 (B), FASTA

>2FF3_3 Actin, alpha skeletal muscle (chains B)
DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMT
QIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDL
AGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSY
ELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMS
GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQ
EYDEAGPSIVHRKCF

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	3
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1

Primary citation

The Structural Basis of Actin Interaction with Multiple WH2/beta-Thymosin Motif-Containing Proteins. Aguda, A.H., Xue, B., Irobi, E. et al. Structure (2006) 14:469-476. DOI 10.1016/j.str.2005.12.011 · PubMed

Other PDB entries of the same protein (UniProt P06396 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5FAF 1.05 Å, N184K pathological variant of gelsolin domain 2 (orthorhombic form)
6QW3 1.3 Å, Calcium-bound gelsolin domain 2
6LJE 1.4 Å, Crystal structure of gelsolin G3 domain (calcium and magnesium condition)
6LJF 1.5 Å, Crystal structure of gelsolin G3 domain (calcium condition)
2FH1 1.55 Å, C-terminal half of gelsolin soaked in low calcium at pH 4.5
3CIP 1.6 Å, Complex of Dictyostelium Discoideum Actin with Gelsolin
1KCQ 1.65 Å, Human Gelsolin Domain 2 with a Cd2+ bound
3CI5 1.7 Å, Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin
5FAE 1.7 Å, N184K pathological variant of gelsolin domain 2 (trigonal form)
5O2Z 1.7 Å, Domain swap dimer of the G167R variant of gelsolin second domain
1D4X 1.75 Å, Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin…
1NLV 1.8 Å, Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human…

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