3L4Q: Non-structural protein 1

Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein. Determined by X-ray diffraction at 2.3 Å resolution. Released 2 Feb 2010.

Method: X-ray diffraction
Resolution: 2.3 Å
Organisms: Influenza A virus, Bos taurus
Chains: 4
Atoms: 4,923
Mol. weight: 78.36 kDa
Released: 2 Feb 2010

Explore 3L4Q in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3L4Q contains 13 α-helices and 14 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	88-91	4	1
α-helix	95-99	5
β-strand	107-112	6	1
β-strand	115-120	6	1
β-strand	127-137	11	1
β-strand	140-151	12	1
β-strand	156-162	7	1
α-helix	171-186	16
β-strand	191-194	4	1
α-helix	196-201	6

Chain B: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	87-91	5	2
α-helix	95-99	5
β-strand	107-112	6	2
β-strand	115-120	6	2
β-strand	127-137	11	2
β-strand	140-151	12	2
β-strand	156-162	7	2
α-helix	171-187	17
β-strand	191-194	4	2
α-helix	196-201	6

Chain C: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	430-492	63
α-helix	498-500	3
α-helix	517-577	61
α-helix	582-589	8

Chain D: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	431-495	65
α-helix	518-577	60
α-helix	582-589	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Non-structural protein 1	A, B	protein	164	Influenza A virus	P03496 (AlphaFold model)
Phosphatidylinositol 3-kinase regulatory subunit beta	C, D	protein	170	Bos taurus	P23726 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>3L4Q_1 Non-structural protein 1 (chains A, B)
HHHHHHSDEALKMTMASVPASRYLTDMTLEEMSRDWSMLIPKQKVAGPLCIRMDQAIMDK
NIILKANFSVIFDRLETLILLRAFTEEGAIVGEISPLPSLPGHTAEDVKNAVGVLIGGLE
WNDNTVRVSETLQRFAWRSSNENGRPPLTPKQKREMAGTIRSEV

Sequence of entity 2 (C, D), FASTA

>3L4Q_2 Phosphatidylinositol 3-kinase regulatory subunit beta (chains C, D)
YQQDQIVKEDSVEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNET
IKIFEEQGQTQEKSSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQELR
AQASDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGARQKKINEWLGI

Primary citation

Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein. Hale, B.G., Kerry, P.S., Jackson, D. et al. Proc Natl Acad Sci U S A (2010) 107:1954-1959. DOI 10.1073/pnas.0910715107 · PubMed

Other PDB entries of the same protein (UniProt P03496 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2ZKO 1.7 Å, Structural basis for dsRNA recognition by NS1 protein of human influenza virus A
3RVC 1.8 Å, Effector domain of NS1 from influenza A/PR/8/34 containing a W187A mutation
3O9R 2.0 Å, Effector domain of NS1 from influenza A/PR/8/34 containing a W187A mutation
2GX9 2.1 Å, X-ray structure of influenza virus NS1 effector domain
3O9T 2.2 Å, Effector domain from influenza A/PR/8/34 NS1
3O9S 2.48 Å, Effector domain of influenza A/PR/8/34 NS1
3O9Q 2.5 Å, Effector domain of NS1 from A/PR/8/34 containing a W187A mutation
5NT1 2.82 Å, Complex of influenza A NS1 effector domain with TRIM25 coiled coil
3O9U 3.2 Å, Effector domain of influenza A/PR/8/34 NS1
5NT2 4.26 Å, Complex of influenza A NS1 with TRIM25 coiled coil domain

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