3UM3: Brox Bro1 domain

Crystal structure of the Brox Bro1 domain in complex with the C-terminal tail of CHMP4B. Determined by X-ray diffraction at 3.8 Å resolution. Released 18 Apr 2012.

Method: X-ray diffraction
Resolution: 3.8 Å
Organism: Homo sapiens
Chains: 2
Atoms: 3,154
Mol. weight: 58.22 kDa
Released: 18 Apr 2012

Explore 3UM3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3UM3 contains 21 α-helices and 5 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 20 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	8-11	4
β-strand	13	1	1
α-helix	21-23	3
α-helix	27-47	21
α-helix	54-68	15
α-helix	69-71	3
β-strand	73	1	2
β-strand	81	1	2
β-strand	90-92	3	1
β-strand	102-104	3	1
α-helix	107-130	24
α-helix	137-156	20
α-helix	157-161	5
α-helix	162-164	3
α-helix	168-170	3
α-helix	177-201	25
α-helix	206-226	21
α-helix	232-263	32
α-helix	267-293	27
α-helix	307-326	20
α-helix	327-331	5
α-helix	339-342	4
α-helix	352-355	4
α-helix	358-362	5
α-helix	367-371	5

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	209-221	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
BRO1 domain-containing protein BROX	A	protein	411	Homo sapiens	Q5VW32 (AlphaFold model)
Charged multivesicular body protein 4b	B	protein	104	Homo sapiens	Q9H444 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3UM3_1 BRO1 domain-containing protein BROX (chains A)
DTHWFHRNPLKATAPVSFNYYGVVTGPSASKICNDLRSSRARLLELFTDLSCNPEMMKNA
ADSYFSLLQGFINSLDESTQESKLRYIQNFKWTDTLQGQVPSAQQDAVFELISMGFNVAL
WYTKYASRLAGKENITEDEAKEVHRSLKIAAGIFKHLKESHLPKLITPAEKGRDLESRLI
EAYVIQCQAEAQEVTIARAIELKHAPGLIAALAYETANFYQKADHTLSSLEPAYSAKWRK
YLHLKMCFYTAYAYCYHGETLLASDKCGEAIRSLQEAEKLYAKAEALCKEYGETKGPGPT
VKPSGHLFFRKLGNLVKNTLEKCQRENGFIYFQKIPTEAPQLELKANYGLVEPIPFEFPP
TSVQWTPETLAAFDLTKRPKDDSTKPKPEEEVKPVKEPDIKPQKDTGCYIS

Sequence of entity 2 (B), FASTA

>3UM3_2 Charged multivesicular body protein 4b (chains B)
DNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNL
LEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM

Primary citation

Two Distinct Binding Modes Define the Interaction of Brox with the C-Terminal Tails of CHMP5 and CHMP4B. Mu, R., Dussupt, V., Jiang, J. et al. Structure (2012) 20:887-898. DOI 10.1016/j.str.2012.03.001 · PubMed

Other PDB entries of the same protein (UniProt Q5VW32 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3R9M 1.95 Å, Crystal structure of the Brox Bro1 domain
3ZXP 2.5 Å, Structural and Functional Analyses of the Bro1 Domain Protein BROX
3UM2 2.59 Å, Crystal structure of the Brox Bro1 domain in complex with the C-terminal tail of CHMP5
3ULY 2.6 Å, Crystal Structure of BROX Bro1 Domain in Complex with the C-Terminal Tails of CHMP5
3UM1 2.71 Å, Crystal structure of the Brox Bro1 domain in complex with the C-terminal tail of CHMP5
3UM0 3.1 Å, Crystal structure of the Brox Bro1 domain in complex with the C-terminal tail of CHMP5

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