Crystal Structure of type VI peptidoglycan muramidase effector Tse3 in complex with its cognate immunity protein Tsi3. Determined by X-ray diffraction at 1.9 Å resolution. Released 2 Oct 2013.
Explore 3WA5 in 3D Show helices and sheets RCSB PDB PDBe
3WA5 contains 33 α-helices and 14 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 3-10 | 8 | |
| α-helix | 23-35 | 13 | |
| α-helix | 39-49 | 11 | |
| α-helix | 51-58 | 8 | |
| α-helix | 62-75 | 14 | |
| α-helix | 80-90 | 11 | |
| α-helix | 101-103 | 3 | |
| α-helix | 108-125 | 18 | |
| α-helix | 147-150 | 4 | |
| α-helix | 154-156 | 3 | |
| α-helix | 158-161 | 4 | |
| α-helix | 162-169 | 8 | |
| α-helix | 173-178 | 6 | |
| α-helix | 186-191 | 6 | |
| α-helix | 195-207 | 13 | |
| β-strand | 209 | 1 | 1 |
| α-helix | 210 | 1 | |
| α-helix | 215-217 | 3 | |
| α-helix | 222-223 | 2 | |
| β-strand | 224 | 1 | 1 |
| α-helix | 225-236 | 12 | |
| α-helix | 240-252 | 13 | |
| α-helix | 256-267 | 12 | |
| β-strand | 275-276 | 2 | 2 |
| β-strand | 281-282 | 2 | 2 |
| α-helix | 283-288 | 6 | |
| α-helix | 298-303 | 6 | |
| α-helix | 306-313 | 8 | |
| α-helix | 316-335 | 20 | |
| α-helix | 339-341 | 3 | |
| α-helix | 343-348 | 6 | |
| α-helix | 355-358 | 4 | |
| α-helix | 361-363 | 3 | |
| α-helix | 366-377 | 12 | |
| α-helix | 389-400 | 12 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 24-29 | 6 | 3 |
| β-strand | 34-39 | 6 | 3 |
| β-strand | 42-46 | 5 | 4 |
| β-strand | 50-55 | 6 | 4 |
| β-strand | 64-70 | 7 | 4 |
| α-helix | 73-75 | 3 | |
| β-strand | 79-83 | 5 | 4 |
| β-strand | 86-98 | 13 | 4 |
| β-strand | 101-112 | 12 | 4 |
| β-strand | 115-125 | 11 | 4 |
| α-helix | 133-141 | 9 | |
| β-strand | 143-144 | 2 | 3 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Type VI secretion exported 3 | A | protein | 416 | Pseudomonas aeruginosa | Q9HYC5 (AlphaFold model) |
| Tse3-specific immunity protein | B | protein | 153 | Pseudomonas aeruginosa | Q9HYC4 (AlphaFold model) |
>3WA5_1 Type VI secretion exported 3 (chains A) MTATSDLIESLISYSWDDWQVTRQEARRVIAAIRNDNVPDATIAALDKSGSLIKLFQRVG PPELARSLIASIAGRTTMQRYQARNALIRSLINNPLGTQTDNWIYFPTITFFDICADLAD AAGRLGFAAAGATGVASQAIQGPFSGVGATGVNPTDLPSIAFGDQLKLLNKDPATVTKYS NPLGDLGAYLSQLSPQDKLNQAQTLVGQPISTLFPDAYPGNPPSRAKVMSAAARKYDLTP QLIGAIILAEQRDQTRDEDAKDYQAAVSIKSANTSIGLGQVVVSTAIKYELFTDLLGQPV RRGLSRKAVATLLASDEFNIFATARYIRYVANLASQQDLRKLPKTRGAFPSIDLRAYAGN PRNWPRDNVRALASEYTSRPWDDNLSPGWPMFVDDAYATFLDPGMRFPLEHHHHHH
>3WA5_2 Tse3-specific immunity protein (chains B) MKTVALILASLALLACTAESGVDFDKTLTHPNGLVVERPVGFDARRSAEGFRFDEGGKLR NPRQLEVQRQDAPPPPDLASRRLGDGEARYKVEEDDGGSAGSEYRLWAAKPAGARWIVVS ASEQSEDGEPTFALAWALLERARLQLEHHHHHH
| ID | Name | Formula | Copies |
|---|---|---|---|
| CA | Calcium ion | Ca | 3 |
Water and common crystallization additives (EDO) are not listed.
Complex structure of type VI peptidoglycan muramidase effector and a cognate immunity protein. Wang, T., Ding, J., Zhang, Y. et al. Acta Crystallogr D Biol Crystallogr (2013) 69:1889-1900. DOI 10.1107/S090744491301576X · PubMed
Other PDB entries of the same protein (UniProt Q9HYC5 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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