Immune protein Tsi3 (tsi3) is a 145-residue protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9HYC4.
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The mean pLDDT of this model is 90.0 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 85% |
| 70 to 90 | Confident: backbone generally right | 1% |
| 50 to 70 | Low: treat with caution | 5% |
| Below 50 | Very low: often disordered regions | 10% |
What pLDDT means and how to read it
Immunity protein that plays a role in preventing early activation of toxin Tse3. Occupies Tse3 substrate binding site and prevents the substrate from entering
Forms a heterotetramer with Tse3 consisting of two Tse3 dimers and two Tsi3 dimers. Formation of the complex inactivates Tse3 enzymatic activity
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 4LUQ | X-ray | 1.77 Å | C/D=23-145 |
| 3WA5 | X-ray | 1.9 Å | B=1-145 |
| 4N7S | X-ray | 2.1 Å | B/D=27-145 |
| 4N80 | X-ray | 2.4 Å | B=23-144 |
| 4M5F | X-ray | 2.5 Å | B=21-145 |
| 4N88 | X-ray | 2.8 Å | B/D=27-145 |
| 8IK0 | EM | 3.3 Å | A/B/C/D/E/F/G/H=22-145 |
| 8IK3 | EM | 3.3 Å | A/B/C/D/E/F/G/H=22-145 |
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