4AYI: Complement factor H

Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 Wild type. Determined by X-ray diffraction at 2.31 Å resolution. Released 7 Nov 2012.

Method: X-ray diffraction
Resolution: 2.31 Å
Organisms: HOMO SAPIENS, NEISSERIA MENINGITIDIS MC58
Chains: 3
Atoms: 4,013
Mol. weight: 58.4 kDa
Released: 7 Nov 2012

Explore 4AYI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4AYI contains 19 α-helices and 47 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	325	1	1
β-strand	333-335	3	2
α-helix	338-341	4
α-helix	342-344	3
β-strand	347	1	1
β-strand	352-357	6	2
β-strand	361-362	2	3
β-strand	369-375	7	2
β-strand	378-380	3	2
β-strand	386-387	2	3
β-strand	388-390	3	4
α-helix	391-393	3
β-strand	397	1	5
β-strand	405-407	3	4
β-strand	411-413	3	6
β-strand	416	1	5
α-helix	417	1
α-helix	423-425	3
β-strand	428-432	5	6
β-strand	435-437	3	6

Chain D: 9 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	82-86	5
α-helix	88-89	2
β-strand	98-99	2	7
α-helix	102-104	3
α-helix	106-108	3
β-strand	110-115	6	8
β-strand	118-123	6	8
β-strand	128-129	2	7
α-helix	130-132	3
α-helix	134	1
β-strand	138-149	12	8
β-strand	152-165	14	8
β-strand	169-180	12	8
β-strand	188-201	14	8
β-strand	203	1	9
α-helix	204	1
β-strand	205	1	10
α-helix	206-208	3
β-strand	214-223	10	9
β-strand	226-236	11	9
β-strand	241-247	7	9
α-helix	255	1
β-strand	256-265	10	9
β-strand	270	1	10
β-strand	271-279	9	9
β-strand	282-292	11	9
β-strand	298-307	10	9
β-strand	310-319	10	9

Chain E: 5 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	333-335	3	11
α-helix	338-341	4
α-helix	342-344	3
β-strand	352-357	6	11
β-strand	361-362	2	12
β-strand	369-375	7	11
β-strand	378-380	3	11
β-strand	386-387	2	12
β-strand	388-390	3	13
α-helix	391-393	3
β-strand	397	1	14
β-strand	405-407	3	13
β-strand	411-413	3	15
β-strand	416	1	14
α-helix	417	1
α-helix	423-425	3
β-strand	428-432	5	15
β-strand	435-437	3	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Complement factor H	A, E	protein	125	HOMO SAPIENS	P08603 (AlphaFold model)
Lipoprotein GNA1870 ccompnd 7	D	protein	270	NEISSERIA MENINGITIDIS MC58	Q19KF7 (AlphaFold model)

Sequence of entity 1 (A, E), FASTA

>4AYI_1 COMPLEMENT FACTOR H (chains A, E)
MGTLKPCDYPDIKHGGLYHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDHIHCTQDG
WSPAVPCLRKCYFPYLENGYNQNHGRKFVQGKSIDVACHPGYALPKAQTTVTCMENGWSP
TPRCI

Sequence of entity 2 (D), FASTA

>4AYI_2 LIPOPROTEIN GNA1870 CCOMPND 7 (chains D)
MGPDSDRLQQRRVAADIGTGLADALTAPLDHKDKGLKSLTLEDSIPQNGTLTLSAQGAEK
TFKAGDKDNSLNTGKLKNDKISRFDFVQKIEVDGQTITLASGEFQIYKQNHSAVVALQIE
KINNPDKTDSLINQRSFLVSGLGGEHTAFNQLPGGKAEYHGKAFSSDDPNGRLHYSIDFT
KKQGYGRIEHLKTLEQNVELAAAELKADEKSHAVILGDTRYGSEEKGTYHLALFGDRAQE
IAGSATVKIGEKVHEIGIAGKQLEHHHHHH

Primary citation

Design and Evaluation of Meningococcal Vaccines Through Structure-Based Modification of Host and Pathogen Molecules. Johnson, S., Tan, L., Van Der Veen, S. et al. PLoS Pathog (2012) 8:2981. DOI 10.1371/JOURNAL.PPAT.1002981 · PubMed

Other PDB entries of the same protein (UniProt P08603 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4K12 1.08 Å, Structural Basis for Host Specificity of Factor H Binding by Streptococcus pneumoniae
3R62 1.52 Å, Structure of complement regulator Factor H mutant, T1184R.
3KZJ 1.65 Å, Structure of complement Factor H variant R1203A
2G7I 1.75 Å, Structure of Human Complement Factor H Carboxyl Terminal Domains 19-20: a Basis for…
3SW0 1.8 Å, Structure of the C-terminal region (modules 18-20) of complement regulator Factor H
6ATG 1.8 Å, Insights to complement factor H recruitment by the borrelial CspZ protein as revealed by…
9MLU 1.82 Å, FbaA with Factor H 6-7 domain
9MMX 1.9 Å, M6 protein with Factor H 6-7 domain
3KXV 2.0 Å, Structure of complement Factor H variant Q1139A
3OXU 2.1 Å, Complement components factor H CCP19-20 and C3d in complex
4ONT 2.15 Å, Ternary host recognition complex of complement factor H, C3d, and sialic acid
6ZH1 2.2 Å, Crystal structure of complex between FH19-20 and FhbA protein from Borrelia hermsii

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