4CK7: Tubulin alpha-1D chain

Pseudo-atomic model of microtubule-bound human kinesin-5 motor domain in presence of adp.alfx (NECK-LINKER IN ITS DISCONNECTED CONFORMATION, based on cryo-electron microscopy experiment. Determined by electron microscopy at 9.2 Å resolution. Released 5 Feb 2014.

Method

Electron microscopy

Resolution

9.2 Å

Organisms

BOS TAURUS, HOMO SAPIENS

Chains

3

Atoms

1,354

Mol. weight

144.2 kDa

Ligands

ADP, AF3, TA1, GDP

Released

5 Feb 2014

Explore 4CK7 in 3D Show helices and sheets RCSB PDB PDBe

Molecules and chains

>4CK7_1 TUBULIN ALPHA-1D CHAIN (chains A)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFSVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRGHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYEPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEEGEEY

>4CK7_2 TUBULIN BETA-2B CHAIN (chains B)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM
AACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEEGEDEA

>4CK7_3 KINESIN-LIKE PROTEIN KIF11 (chains C)
MASQPNSSAKKKEEKGKNIQVVVRVRPFNLAERKASAHSIVESDPVRKEVSVRTGGLADK
SSRKTYTFDMVFGASTKQIDVYRSVVAPILDEVIMGYNATIFAYGQTGTGKTFTMEGERS
PNEEYCWEEDPLAGIIPRTLHQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLNPSSDVSE
RLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFS
VTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVIT
ALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNIL
NKPEVNQHHHHHH

Ligands and cofactors

Primary citation

Comprehensive Structural Model of the Mechanochemical Cycle of a Mitotic Motor Highlights Molecular Adaptations in the Kinesin Family. Goulet, A., Major, J., Jun, Y. et al. Proc Natl Acad Sci U S A (2014) 111:1837. DOI 10.1073/PNAS.1319848111 · PubMed

Related structures

Other PDB entries of the same protein (UniProt Q2HJ86 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 1TVK 2.89 Å, The binding mode of epothilone A on a,b-tubulin by electron crystallography
• 7RRO 3.4 Å, Structure of the 48-nm repeat doublet microtubule from bovine tracheal cilia
• 9CPB 3.52 Å, Atomic model of bovine Fallopian tube cilia doublet microtubule (48-nm periodicity)
• 1SA0 3.58 Å, Tubulin-colchicine: stathmin-like domain complex
• 1Z2B 4.1 Å, Tubulin-colchicine-vinblastine: stathmin-like domain complex
• 1SA1 4.2 Å, Tubulin-podophyllotoxin: stathmin-like domain complex
• 2XRP 8.2 Å, Human Doublecortin N-DC Repeat (1MJD) and Mammalian Tubulin (1JFF and 3HKE) Docked into…
• 4ATX 8.2 Å, Rigor kinesin motor domain with an ordered neck-linker, docked on tubulin dimer,…
• 4ATU 8.3 Å, Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM…
• 3IZ0 8.6 Å, Human Ndc80 Bonsai Decorated Microtubule
• 4CK6 9.2 Å, Pseudo-atomic model of microtubule-bound human kinesin-5 motor domain in the ADP.AlFx…
• 5M5I 9.3 Å, Pseudo-atomic model of microtubule-bound S.pombe kinesin-5 motor domain in the AMPPNP…

Browse structure collections

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