4NIF: Heterodimeric structure of ERK2 and RSK1

Heterodimeric structure of ERK2 and RSK1. Determined by X-ray diffraction at 2.15 Å resolution. Released 12 Nov 2014.

Method

X-ray diffraction

Resolution

2.15 Å

Organism

Homo sapiens

Chains

4

Atoms

11,667

Mol. weight

159.51 kDa

Ligands

ANP

Released

12 Nov 2014

Explore 4NIF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4NIF contains 79 α-helices and 52 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 18 helices, 13 β-strands

Molecules and chains

>4NIF_1 Ribosomal protein S6 kinase alpha-1 (chains A, D)
GSNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQ
HPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHS
QGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKR
QGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETA
KDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSS
KPTPQLKPIESSILAQRRVRKLPSTTLHHHHHH

>4NIF_2 Mitogen-activated protein kinase 1 (chains B, E)
GSMAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISP
FEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKT
QHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDH
DHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLN
HILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNP
HKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGY
RS

Ligands and cofactors

Water and common crystallization additives (NA, SO4) are not listed.

Primary citation

Structural assembly of the signaling competent ERK2-RSK1 heterodimeric protein kinase complex. Alexa, A., Gogl, G., Glatz, G. et al. Proc Natl Acad Sci U S A (2015) 112:2711-2716. DOI 10.1073/pnas.1417571112 · PubMed

Related structures

Other PDB entries of the same protein (UniProt Q15418 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 5V62 1.9 Å, Phospho-ERK2 bound to bivalent inhibitor SBP3
• 7P74 1.9 Å, The PDZ domain of SYNJ2BP complexed with the phosphorylated PDZ-binding motif of RSK1
• 2Z7Q 2.0 Å, Crystal structure of the N-terminal kinase domain of human RSK-1 bound to AMP-PCP
• 2Z7R 2.0 Å, Crystal Structure of the N-terminal Kinase Domain of Human RSK1 bound to Staurosporine
• 2Z7S 2.1 Å, Crystal Structure of the N-terminal Kinase Domain of Human RSK1 bound to Purvalnol A
• 5CSI 2.13 Å, S100B-RSK1 crystal structure A'
• 5V61 2.2 Å, Phospho-ERK2 bound to bivalent inhibitor SBP2
• 4H3P 2.3 Å, Crystal structure of human ERK2 complexed with a MAPK docking peptide
• 5N7D 2.3 Å, MAGI-1 complexed with a RSK1 peptide
• 5N7F 2.3 Å, MAGI-1 complexed with a pRSK1 peptide
• 6TWY 2.3 Å, MAGI1_2 complexed with a phosphomimetic RSK1 peptide
• 2WNT 2.4 Å, Crystal Structure of the Human Ribosomal protein S6 kinase

Browse structure collections

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