5E6A: Glucocorticoid receptor

Glucocorticoid receptor DNA binding domain - PLAU NF-kB response element complex. Determined by X-ray diffraction at 2.2 Å resolution. Released 8 Feb 2017.

Method: X-ray diffraction
Resolution: 2.2 Å
Organism: Homo sapiens
Chains: 4
Atoms: 1,825
Mol. weight: 35.58 kDa
Ligands: ZN
Released: 8 Feb 2017

Explore 5E6A in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5E6A contains 7 α-helices and 6 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	427-428	2
β-strand	430-432	3	1
β-strand	435-437	3	1
α-helix	439-450	12
α-helix	474-483	10

Chain B: 4 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	420	1	2
β-strand	427	1	2
α-helix	428	1
β-strand	430-432	3	3
β-strand	435-437	3	3
α-helix	439-450	12
α-helix	469-471	3
α-helix	474-483	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Glucocorticoid receptor	A, B	protein	114	Homo sapiens	P04150 (AlphaFold model)
DNA (5'-d(cptpgpgpgpapaptptptpcpcptpgpapt)-3')	C	DNA	16	Homo sapiens
DNA (5'-d(aptpcpapgpgpapapaptptpcpcpcpapg)-3')	D	DNA	16	Homo sapiens

Sequence of entity 1 (A, B), FASTA

>5E6A_1 Glucocorticoid receptor (chains A, B)
MHHHHHHSSGVDLGTENLYFQSNAPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQ
HNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTG

Sequence of entity 2 (C), FASTA

>5E6A_2 DNA (5'-D(*CP*TP*GP*GP*GP*AP*AP*TP*TP*TP*CP*CP*TP*GP*AP*T)-3') (chains C)
CTGGGAATTTCCTGAT

Sequence of entity 3 (D), FASTA

>5E6A_3 DNA (5'-D(*AP*TP*CP*AP*GP*GP*AP*AP*AP*TP*TP*CP*CP*CP*AP*G)-3') (chains D)
ATCAGGAAATTCCCAG

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	4

Primary citation

Cryptic glucocorticoid receptor-binding sites pervade genomic NF-kappa B response elements. Hudson, W.H., Vera, I.M.S., Nwachukwu, J.C. et al. Nat Commun (2018) 9:1337-1337. DOI 10.1038/s41467-018-03780-1 · PubMed

Other PDB entries of the same protein (UniProt P04150 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4UDD 1.8 Å, GR in complex with desisobutyrylciclesonide
5E69 1.85 Å, Glucocorticoid receptor DNA binding domain - IL8 NF-kB response element complex
4HN5 1.9 Å, GR DNA Binding Domain - TSLP nGRE Complex
4P6W 1.95 Å, Crystal Structure of mometasone furoate-bound glucocorticoid receptor ligand binding…
8A9G 1.96 Å, Binary complex of 14-3-3 zeta Glucocorticoid Receptor (GR) pT524 peptide stabilised by…
5CBY 2.0 Å, AncGR2 DNA Binding Domain - (+)GRE Complex
5CBX 2.0 Å, AncGR DNA Binding Domain - (+)GRE Complex
6X6E 2.0 Å, Glucocorticoid Receptor DNA binding domain in complex with methylated precursor for a…
5UC3 2.01 Å, Structure of the dominant negative mutant Glucocorticoid Receptor alpha (L733K/N734P)…
6YMO 2.02 Å, Binary complex of 14-3-3 zeta with Glucocorticoid Receptor (GR) pS617 peptide
6YO8 2.09 Å, Binary complex of 14-3-3 zeta with Glucocorticoid Receptor (GR) pT524 peptide
3K22 2.1 Å, Glucocorticoid Receptor with Bound alaninamide 10 with TIF2 peptide

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