6L9D: Immunoglobulin G-binding protein G

X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11S. Determined by X-ray diffraction at 1.73 Å resolution. Released 12 Aug 2020.

Method: X-ray diffraction
Resolution: 1.73 Å
Organism: Streptococcus sp. group G
Chains: 1
Atoms: 451
Mol. weight: 6.14 kDa
Released: 12 Aug 2020

Explore 6L9D in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6L9D contains 1 α-helix and 4 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 4 β-strands

Element	Residues	Length	Sheet
β-strand	2-8	7	1
β-strand	13-19	7	1
α-helix	23-36	14
β-strand	42-46	5	1
β-strand	51-55	5	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Immunoglobulin G-binding protein G	A	protein	56	Streptococcus sp. group G	P06654 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6L9D_1 Immunoglobulin G-binding protein G (chains A)
DTYKLILNGVSLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTE

Primary citation

Increasing protein stability by engineering the n -> pi * interaction at the beta-turn. Khatri, B., Majumder, P., Nagesh, J. et al. Chem Sci (2020) 11:9480-9487. DOI 10.1039/d0sc03060k · PubMed

Other PDB entries of the same protein (UniProt P06654 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1IGD 1.1 Å, The third IgG-binding domain from streptococcal protein G: an analysis by X-ray…
2IGD 1.1 Å, Anisotropic structure of protein G IgG-binding domain III at 1.1 Å resolution
3MP9 1.2 Å, Structure of Streptococcal protein G B1 domain at pH 3.0
6CNE 1.2 Å, Selenomethionine variant (V29SeM) of protein GB1
1PGX 1.66 Å, The 1.66 Å X-ray structure of the B2 immunoglobulin-binding domain of streptococcal…
6L91 1.84 Å, X-ray structure of synthetic GB1 domain with the mutation K10(DVA).
6LJI 1.84 Å, X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11V
1PGB 1.92 Å, Two crystal structures of the B1 immunoglobulin-binding domain of streptoccocal protein…
4KGS 1.95 Å, Backbone Modifications in the Protein GB1 Loops: beta-3-Val21, beta-3-Asp40
6L9B 1.95 Å, X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11A
1EM7 2.0 Å, Helix variant of the B1 domain from streptococcal protein G
4KGR 2.0 Å, Backbone Modifications in the Protein GB1 Helix: beta-3-Ala24, beta-3-Lys28,…

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