6PSU: Protein TraR
Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi2) with TraR and rpsT P2 promoter. Determined by electron microscopy at 3.9 Å resolution. Released 25 Mar 2020.
- Method
- Electron microscopy
- Resolution
- 3.9 Å
- Organism
- Escherichia coli
- Chains
- 10
- Atoms
- 32,167
- Mol. weight
- 562.9 kDa
- Ligands
- ZN, MG, 1N7
- Released
- 25 Mar 2020
Explore 6PSU in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
6PSU contains 181 α-helices and 231 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain G: 8 helices, 21 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-11 | 3 | |
| β-strand | 13 | 1 | 2 |
| β-strand | 17 | 1 | 3 |
| β-strand | 23-25 | 3 | 3 |
| β-strand | 28-31 | 4 | 2 |
| α-helix | 35-49 | 15 | |
| β-strand | 53 | 1 | 4 |
| β-strand | 56-61 | 6 | 5 |
| α-helix | 78-85 | 8 | |
| β-strand | 90-91 | 2 | 6 |
| β-strand | 97-105 | 9 | 5 |
| β-strand | 108-111 | 4 | 7 |
| α-helix | 112-114 | 3 | |
| β-strand | 115 | 1 | 5 |
| α-helix | 116-118 | 3 | |
| β-strand | 122-123 | 2 | 6 |
| β-strand | 129-133 | 5 | 7 |
| β-strand | 139-147 | 9 | 5 |
| β-strand | 149 | 1 | 4 |
| β-strand | 153 | 1 | 8 |
| α-helix | 155-158 | 4 | |
| β-strand | 171-172 | 2 | 5 |
| α-helix | 173-174 | 2 | |
| β-strand | 175 | 1 | 8 |
| β-strand | 180-184 | 5 | 3 |
| β-strand | 187-189 | 3 | 2 |
| β-strand | 199-201 | 3 | 2 |
| β-strand | 204-207 | 4 | 3 |
| α-helix | 213-233 | 21 | |
Chain H: 5 helices, 24 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 16-18 | 3 | 9 |
| β-strand | 24-26 | 3 | 9 |
| β-strand | 27 | 1 | 10 |
| β-strand | 31 | 1 | 11 |
| α-helix | 35-49 | 15 | |
| β-strand | 56-60 | 5 | 12 |
| β-strand | 74 | 1 | 13 |
| α-helix | 78-86 | 9 | |
| β-strand | 90-92 | 3 | 14 |
| β-strand | 97-100 | 4 | 12 |
| β-strand | 104-105 | 2 | 15 |
| β-strand | 109-110 | 2 | 16 |
| β-strand | 121-123 | 3 | 14 |
| β-strand | 131-132 | 2 | 16 |
| β-strand | 133 | 1 | 13 |
| β-strand | 139-140 | 2 | 15 |
| β-strand | 143-147 | 5 | 12 |
| α-helix | 155-157 | 3 | |
| β-strand | 172 | 1 | 12 |
| β-strand | 180-184 | 5 | 9 |
| β-strand | 187 | 1 | 17 |
| β-strand | 190 | 1 | 18 |
| β-strand | 198 | 1 | 18 |
| β-strand | 199 | 1 | 11 |
| β-strand | 201 | 1 | 17 |
| β-strand | 202 | 1 | 10 |
| β-strand | 204-207 | 4 | 9 |
| α-helix | 213-227 | 15 | |
| α-helix | 228-230 | 3 | |
Chain I: 57 helices, 97 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-9 | 5 | |
| β-strand | 13 | 1 | 19 |
| α-helix | 19-20 | 2 | |
| α-helix | 29-35 | 7 | |
| α-helix | 48-56 | 9 | |
| β-strand | 59-60 | 2 | 20 |
| β-strand | 66-75 | 10 | 20 |
| α-helix | 76-78 | 3 | |
| α-helix | 82-88 | 7 | |
| β-strand | 93-106 | 14 | 20 |
| β-strand | 109-116 | 8 | 20 |
| β-strand | 119-128 | 10 | 20 |
| β-strand | 143-144 | 2 | 21 |
| α-helix | 146 | 1 | |
| β-strand | 147-148 | 2 | 22 |
| β-strand | 149-151 | 3 | 23 |
| α-helix | 152 | 1 | |
| β-strand | 156-158 | 3 | 24 |
| β-strand | 173-176 | 4 | 24 |
| β-strand | 180 | 1 | 25 |
| β-strand | 184-187 | 4 | 24 |
| β-strand | 195-197 | 3 | 24 |
| β-strand | 204-205 | 2 | 24 |
| α-helix | 207-212 | 6 | |
| α-helix | 217-224 | 8 | |
| β-strand | 228-231 | 4 | 26 |
| β-strand | 239-240 | 2 | 27 |
| β-strand | 250 | 1 | 28 |
| β-strand | 255-257 | 3 | 29 |
| β-strand | 260-263 | 4 | 29 |
| β-strand | 268 | 1 | 28 |
| α-helix | 271-280 | 10 | |
| β-strand | 284-285 | 2 | 27 |
| β-strand | 297 | 1 | 26 |
| β-strand | 301 | 1 | 30 |
| β-strand | 311 | 1 | 30 |
| α-helix | 319-328 | 10 | |
| β-strand | 332-335 | 4 | 26 |
| α-helix | 347-353 | 7 | |
| α-helix | 360-370 | 11 | |
| α-helix | 378-389 | 12 | |
| α-helix | 392-394 | 3 | |
| β-strand | 396 | 1 | 25 |
| α-helix | 399-408 | 10 | |
| α-helix | 422-436 | 15 | |
| β-strand | 451-453 | 3 | 23 |
| α-helix | 456-480 | 25 | |
| α-helix | 496-507 | 12 | |
| β-strand | 513-514 | 2 | 21 |
| α-helix | 515 | 1 | |
| α-helix | 520-526 | 7 | |
| β-strand | 529 | 1 | 31 |
| β-strand | 530-531 | 2 | 22 |
| α-helix | 552-554 | 3 | |
| β-strand | 558 | 1 | 32 |
| β-strand | 574 | 1 | 31 |
| β-strand | 575 | 1 | 32 |
| β-strand | 580-581 | 2 | 33 |
| β-strand | 587-594 | 8 | 33 |
| β-strand | 599-607 | 9 | 33 |
| α-helix | 608-611 | 4 | |
| β-strand | 616 | 1 | 33 |
| β-strand | 633-637 | 5 | 33 |
| β-strand | 642-646 | 5 | 33 |
| α-helix | 647-649 | 3 | |
| β-strand | 652-654 | 3 | 33 |
| α-helix | 658-660 | 3 | |
| α-helix | 665-667 | 3 | |
| α-helix | 671-673 | 3 | |
| β-strand | 674 | 1 | 34 |
| α-helix | 676-686 | 11 | |
| β-strand | 689 | 1 | 35 |
| α-helix | 690-691 | 2 | |
| β-strand | 700 | 1 | 19 |
| α-helix | 705-710 | 6 | |
| β-strand | 716-717 | 2 | 36 |
| β-strand | 722-727 | 6 | 37 |
| β-strand | 731-736 | 6 | 37 |
| β-strand | 749-752 | 4 | 37 |
| β-strand | 757-759 | 3 | 38 |
| β-strand | 763-765 | 3 | 38 |
| β-strand | 768-769 | 2 | 36 |
| β-strand | 776 | 1 | 37 |
| β-strand | 782-785 | 4 | 36 |
| β-strand | 789-790 | 2 | 39 |
| β-strand | 793-794 | 2 | 39 |
| β-strand | 798-801 | 4 | 40 |
| β-strand | 802-804 | 3 | 41 |
| β-strand | 815-817 | 3 | 42 |
| β-strand | 818 | 1 | 41 |
| α-helix | 820-824 | 5 | |
| β-strand | 832 | 1 | 43 |
| β-strand | 835-836 | 2 | 44 |
| β-strand | 843 | 1 | 45 |
| β-strand | 846 | 1 | 45 |
| β-strand | 849 | 1 | 46 |
| α-helix | 859-862 | 4 | |
| β-strand | 865 | 1 | 47 |
| β-strand | 870 | 1 | 48 |
| β-strand | 871 | 1 | 47 |
| β-strand | 877 | 1 | 49 |
| β-strand | 884 | 1 | 48 |
| β-strand | 887 | 1 | 46 |
| β-strand | 889 | 1 | 50 |
| α-helix | 895-896 | 2 | |
| α-helix | 900-905 | 6 | |
| β-strand | 913 | 1 | 50 |
| β-strand | 926 | 1 | 49 |
| β-strand | 927-931 | 5 | 44 |
| β-strand | 936 | 1 | 51 |
| α-helix | 940-942 | 3 | |
| α-helix | 943-978 | 36 | |
| α-helix | 988-991 | 4 | |
| α-helix | 994-996 | 3 | |
| α-helix | 1006-1037 | 32 | |
| β-strand | 1040 | 1 | 51 |
| α-helix | 1042-1043 | 2 | |
| β-strand | 1052-1055 | 4 | 44 |
| β-strand | 1056 | 1 | 43 |
| β-strand | 1065 | 1 | 42 |
| β-strand | 1075-1078 | 4 | 42 |
| β-strand | 1087-1088 | 2 | 52 |
| α-helix | 1092 | 1 | |
| β-strand | 1093 | 1 | 52 |
| α-helix | 1094 | 1 | |
| β-strand | 1096-1098 | 3 | 41 |
| α-helix | 1102-1105 | 4 | |
| β-strand | 1108 | 1 | 34 |
| α-helix | 1110-1133 | 24 | |
| α-helix | 1139-1149 | 11 | |
| α-helix | 1171-1174 | 4 | |
| β-strand | 1182 | 1 | 19 |
| α-helix | 1192-1201 | 10 | |
| β-strand | 1209-1210 | 2 | 41 |
| β-strand | 1212-1213 | 2 | 52 |
| α-helix | 1219 | 1 | |
| β-strand | 1220 | 1 | 52 |
| α-helix | 1221 | 1 | |
| β-strand | 1225-1226 | 2 | 41 |
| β-strand | 1229-1232 | 4 | 40 |
| β-strand | 1235 | 1 | 35 |
| α-helix | 1239-1241 | 3 | |
| β-strand | 1244-1246 | 3 | 53 |
| β-strand | 1251 | 1 | 54 |
| β-strand | 1252 | 1 | 55 |
| β-strand | 1258 | 1 | 54 |
| α-helix | 1266-1269 | 4 | |
| β-strand | 1270 | 1 | 56 |
| α-helix | 1272-1280 | 9 | |
| α-helix | 1285-1288 | 4 | |
| α-helix | 1289-1293 | 5 | |
| α-helix | 1298-1309 | 12 | |
| α-helix | 1321-1332 | 12 | |
| β-strand | 1335-1339 | 5 | 57 |
Chain J: 62 helices, 81 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 20-24 | 5 | 57 |
| α-helix | 27-33 | 7 | |
| β-strand | 37 | 1 | 58 |
| β-strand | 44 | 1 | 59 |
| β-strand | 51 | 1 | 59 |
| β-strand | 52 | 1 | 60 |
| β-strand | 54 | 1 | 60 |
| β-strand | 58 | 1 | 60 |
| α-helix | 59-62 | 4 | |
| α-helix | 78-80 | 3 | |
| α-helix | 83 | 1 | |
| β-strand | 84 | 1 | 61 |
| α-helix | 85 | 1 | |
| β-strand | 91 | 1 | 61 |
| α-helix | 95-99 | 5 | |
| β-strand | 103-112 | 10 | 58 |
| α-helix | 114-117 | 4 | |
| α-helix | 132-139 | 8 | |
| β-strand | 145 | 1 | 62 |
| β-strand | 159 | 1 | 62 |
| α-helix | 162-169 | 8 | |
| β-strand | 179 | 1 | 62 |
| α-helix | 182-191 | 10 | |
| α-helix | 194-206 | 13 | |
| α-helix | 211-230 | 20 | |
| α-helix | 234-236 | 3 | |
| β-strand | 238-244 | 7 | 58 |
| α-helix | 245-246 | 2 | |
| α-helix | 253 | 1 | |
| β-strand | 254 | 1 | 63 |
| α-helix | 255 | 1 | |
| β-strand | 260 | 1 | 63 |
| β-strand | 261-262 | 2 | 64 |
| α-helix | 264-285 | 22 | |
| α-helix | 289-307 | 19 | |
| α-helix | 328-330 | 3 | |
| α-helix | 337-342 | 6 | |
| β-strand | 345 | 1 | 56 |
| β-strand | 350-352 | 3 | 53 |
| β-strand | 355 | 1 | 65 |
| β-strand | 365-369 | 5 | 66 |
| α-helix | 370-376 | 7 | |
| α-helix | 378-388 | 11 | |
| α-helix | 394-403 | 10 | |
| α-helix | 406-415 | 10 | |
| β-strand | 421-424 | 4 | 53 |
| α-helix | 431-433 | 3 | |
| β-strand | 434-437 | 4 | 53 |
| β-strand | 438-442 | 5 | 66 |
| β-strand | 447 | 1 | 65 |
| α-helix | 451-457 | 7 | |
| β-strand | 467-469 | 3 | 53 |
| α-helix | 474-479 | 6 | |
| α-helix | 480-484 | 5 | |
| β-strand | 485 | 1 | 53 |
| α-helix | 486-488 | 3 | |
| β-strand | 491 | 1 | 67 |
| β-strand | 498 | 1 | 67 |
| α-helix | 506-513 | 8 | |
| β-strand | 526-527 | 2 | 68 |
| α-helix | 530-538 | 9 | |
| β-strand | 547-554 | 8 | 68 |
| β-strand | 566-573 | 8 | 68 |
| α-helix | 574-580 | 7 | |
| α-helix | 589-591 | 3 | |
| α-helix | 598-612 | 15 | |
| α-helix | 615-635 | 21 | |
| α-helix | 641-643 | 3 | |
| α-helix | 645-647 | 3 | |
| α-helix | 650-669 | 20 | |
| α-helix | 675-702 | 28 | |
| β-strand | 706-707 | 2 | 69 |
| β-strand | 714-715 | 2 | 69 |
| α-helix | 723-725 | 3 | |
| α-helix | 734-741 | 8 | |
| α-helix | 769-786 | 18 | |
| α-helix | 795-804 | 10 | |
| β-strand | 809 | 1 | 70 |
| β-strand | 820-822 | 3 | 71 |
| α-helix | 835-839 | 5 | |
| β-strand | 843 | 1 | 72 |
| β-strand | 844 | 1 | 71 |
| β-strand | 849 | 1 | 73 |
| β-strand | 856 | 1 | 73 |
| β-strand | 863 | 1 | 72 |
| α-helix | 866-875 | 10 | |
| β-strand | 880-882 | 3 | 71 |
| β-strand | 894 | 1 | 70 |
| α-helix | 896-899 | 4 | |
| α-helix | 915-924 | 10 | |
| α-helix | 926-929 | 4 | |
| β-strand | 951-952 | 2 | 74 |
| β-strand | 956 | 1 | 75 |
| β-strand | 957-958 | 2 | 76 |
| β-strand | 961 | 1 | 76 |
| β-strand | 965-967 | 3 | 77 |
| β-strand | 973-975 | 3 | 77 |
| β-strand | 981-985 | 5 | 76 |
| β-strand | 993-996 | 4 | 76 |
| β-strand | 1002-1003 | 2 | 77 |
| β-strand | 1011 | 1 | 75 |
| β-strand | 1015-1016 | 2 | 74 |
| β-strand | 1021 | 1 | 78 |
| β-strand | 1024 | 1 | 78 |
| β-strand | 1025 | 1 | 79 |
| β-strand | 1033-1034 | 2 | 80 |
| β-strand | 1038 | 1 | 81 |
| β-strand | 1042 | 1 | 82 |
| β-strand | 1046 | 1 | 82 |
| β-strand | 1049-1051 | 3 | 83 |
| β-strand | 1056-1058 | 3 | 83 |
| β-strand | 1077-1079 | 3 | 81 |
| β-strand | 1089 | 1 | 84 |
| β-strand | 1096 | 1 | 84 |
| β-strand | 1098-1100 | 3 | 81 |
| β-strand | 1114-1115 | 2 | 80 |
| β-strand | 1124 | 1 | 79 |
| α-helix | 1139-1144 | 6 | |
| β-strand | 1156 | 1 | 85 |
| β-strand | 1161-1162 | 2 | 86 |
| β-strand | 1163-1165 | 3 | 87 |
| β-strand | 1173 | 1 | 88 |
| β-strand | 1175-1177 | 3 | 87 |
| β-strand | 1190 | 1 | 88 |
| α-helix | 1195-1196 | 2 | |
| β-strand | 1203-1204 | 2 | 86 |
| α-helix | 1205 | 1 | |
| β-strand | 1209 | 1 | 85 |
| α-helix | 1215-1216 | 2 | |
| α-helix | 1217-1224 | 8 | |
| α-helix | 1226-1242 | 17 | |
| α-helix | 1251-1260 | 10 | |
| β-strand | 1263-1266 | 4 | 89 |
| β-strand | 1279-1281 | 3 | 89 |
| α-helix | 1282-1294 | 13 | |
| α-helix | 1298-1299 | 2 | |
| β-strand | 1302-1304 | 3 | 89 |
| α-helix | 1309-1314 | 6 | |
| α-helix | 1328-1337 | 10 | |
| β-strand | 1341-1342 | 2 | 57 |
| α-helix | 1347-1353 | 7 | |
| α-helix | 1361-1363 | 3 | |
| α-helix | 1364-1372 | 9 | |
Chain K: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-12 | 6 | |
| α-helix | 18-31 | 14 | |
| α-helix | 46-54 | 9 | |
| α-helix | 61-72 | 12 | |
Chain L: 36 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-19 | 11 | |
| α-helix | 24-29 | 6 | |
| α-helix | 40-48 | 9 | |
| α-helix | 77-83 | 7 | |
| α-helix | 97-105 | 9 | |
| α-helix | 110-112 | 3 | |
| α-helix | 113-136 | 24 | |
| α-helix | 138-152 | 15 | |
| β-strand | 165 | 1 | 90 |
| α-helix | 214-229 | 16 | |
| α-helix | 232-234 | 3 | |
| α-helix | 246-249 | 4 | |
| α-helix | 251-257 | 7 | |
| β-strand | 259 | 1 | 90 |
| α-helix | 263-287 | 25 | |
| α-helix | 299-303 | 5 | |
| α-helix | 306-309 | 4 | |
| α-helix | 319-321 | 3 | |
| α-helix | 327-330 | 4 | |
| α-helix | 337-351 | 15 | |
| α-helix | 355-381 | 27 | |
| α-helix | 384-390 | 7 | |
| α-helix | 401-416 | 16 | |
| α-helix | 421-423 | 3 | |
| α-helix | 427-443 | 17 | |
| α-helix | 454-474 | 21 | |
| α-helix | 480-486 | 7 | |
| α-helix | 494-498 | 5 | |
| α-helix | 503-504 | 2 | |
| β-strand | 505-506 | 2 | 64 |
| α-helix | 509 | 1 | |
| β-strand | 510 | 1 | 91 |
| α-helix | 511 | 1 | |
| β-strand | 518 | 1 | 91 |
| α-helix | 519-522 | 4 | |
| β-strand | 524 | 1 | 55 |
| α-helix | 529-530 | 2 | |
| α-helix | 531-549 | 19 | |
| α-helix | 553-562 | 10 | |
| α-helix | 573-579 | 7 | |
| α-helix | 584-598 | 15 | |
| α-helix | 605-608 | 4 | |
Chain M: 6 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 251-253 | 3 | |
| α-helix | 257-259 | 3 | |
| α-helix | 264-272 | 9 | |
| α-helix | 278-281 | 4 | |
| α-helix | 286-290 | 5 | |
| α-helix | 297-309 | 13 | |
Chain N: 3 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-25 | 22 | |
| β-strand | 36-37 | 2 | 1 |
| β-strand | 42-43 | 2 | 1 |
| α-helix | 46-51 | 6 | |
| α-helix | 59-70 | 12 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Protein TraR | N | protein | 72 | Escherichia coli | P41065 (AlphaFold model) |
| DNA-directed RNA polymerase subunit alpha | G, H, M | protein | 329 | Escherichia coli | P0A7Z4 (AlphaFold model) |
| DNA-directed RNA polymerase subunit beta | I | protein | 1342 | Escherichia coli | P0A8V2 (AlphaFold model) |
| DNA-directed RNA polymerase subunit beta' | J | protein | 1430 | Escherichia coli | P0A8T7 (AlphaFold model) |
| DNA-directed RNA polymerase subunit omega | K | protein | 91 | Escherichia coli | P0A800 |
| RNA polymerase sigma factor RpoD | L | protein | 616 | Escherichia coli | P00579 |
| DNA (85-mer) | O | DNA | 85 | Escherichia coli | |
| DNA (85-mer) | P | DNA | 85 | Escherichia coli | |
Sequence of entity 1 (N), FASTA
>6PSU_1 Protein TraR (chains N)
SDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPIPEARRKIFPGVTLCVEC
QAYQERQRKHYA
Sequence of entity 2 (G, H, M), FASTA
>6PSU_2 DNA-directed RNA polymerase subunit alpha (chains G, H, M)
MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
IDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGD
VEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPV
ERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSL
TEIKDVLASRGLSLGMRLENWPPASIADE
Sequence of entity 3 (I), FASTA
>6PSU_3 DNA-directed RNA polymerase subunit beta (chains I)
MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQ
SYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQ
EVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYR
GSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKD
YIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRL
SALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGIL
SKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTR
ERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVT
DEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQV
VSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERG
DVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEK
LLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDE
LKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKIN
PIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAK
LREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLK
LGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE
Sequence of entity 4 (J), FASTA
>6PSU_4 DNA-directed RNA polymerase subunit beta' (chains J)
VKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCAR
IFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELASPTAHIWFLKSL
PSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGDEFDAKM
GAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQ
EAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPY
LRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEVIREHP
VLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA
RALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSG
LASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKA
ISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAE
VAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFN
SIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGAR
KGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVL
GRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYG
RDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKL
SNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANW
DPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKI
VDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPR
VADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFE
GERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQM
LRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPA
APQVTAEDASASLAELLNAGLGGSDNELELEVLFQGPSSGHHHHHHHHHH
Sequence of entity 5 (K), FASTA
>6PSU_5 DNA-directed RNA polymerase subunit omega (chains K)
MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLIN
NQILDVRERQEQQEQEAAELQAVTAIAEGRR
Sequence of entity 6 (L), FASTA
>6PSU_6 RNA polymerase sigma factor RpoD (chains L)
SEFMEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVME
EAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEI
DIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLITGFVDPNAEEDLAPT
ATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAREKFAELRAQYVVTRDTIKA
KGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKM
PKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQ
VKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVD
KFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGRE
PTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATT
ESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRK
LRHPSRSEVLRSFLDD
Sequence of entity 7 (O), FASTA
>6PSU_7 DNA (85-MER) (chains O)
GGCGGCGCTTATTTGCACAAATCCATTGACAAAAGAAGGCTAAAAGGGCATATTCCTCGG
CCTTTGAATTGTCCATATAGAACGC
Sequence of entity 8 (P), FASTA
>6PSU_8 DNA (85-MER) (chains P)
GCGTTCTATATGGACAATTCAAAGGCCGAGGAATATGCCCTTTTAGCCTTCTTTTGTCAA
TGGATTTGTGCAAATAAGCGCCGCC
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 3 |
| MG | Magnesium ion | Mg | 1 |
| 1N7 | Chapso | C32 H59 N2 O8 S | 4 |
Primary citation
Stepwise Promoter Melting by Bacterial RNA Polymerase. Chen, J., Chiu, C., Gopalkrishnan, S. et al. Mol Cell (2020) 78:275. DOI 10.1016/j.molcel.2020.02.017 · PubMed
Other PDB entries of the same protein (UniProt P41065 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 6PST 3.0 Å, Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi1.5b) with TraR and…
- 6PSQ 3.4 Å, Escherichia coli RNA polymerase closed complex (TRPc) with TraR and rpsT P2 promoter
- 6PSR 3.4 Å, Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi1) with TraR and…
- 6PSS 3.5 Å, Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi1.5a) with TraR and…
- 6PSV 3.5 Å, Escherichia coli RNA polymerase promoter unwinding intermediate (TpreRPo) with TraR and…
- 6N57 3.7 Å, Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation I
- 6PSW 3.7 Å, Escherichia coli RNA polymerase promoter unwinding intermediate (TRPo) with TraR and…
- 6N58 3.78 Å, Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation II
- 5W1S 3.81 Å, X-ray crystal structure of Escherichia coli RNA polymerase and TraR complex
Browse structure collections
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