6SKL: DNA replication licensing factor MCM2

Name: PDB 6SKL: Cryo-EM structure of the CMG Fork Protection Complex at a replication fork - Conformation 1
Published: 2020-05-06
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: replication

Cryo-EM structure of the CMG Fork Protection Complex at a replication fork - Conformation 1. Determined by electron microscopy at 3.7 Å resolution. Released 6 May 2020.

Method: Electron microscopy
Resolution: 3.7 Å
Organisms: Saccharomyces cerevisiae (strain ATCC 204508 / S288c), synthetic construct
Chains: 18
Atoms: 59,184
Mol. weight: 1326.61 kDa
Ligands: ZN, ANP, MG
Released: 6 May 2020

Explore 6SKL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6SKL contains 383 α-helices and 301 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 34 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	183-186	4
α-helix	194-198	5
α-helix	201-217	17
α-helix	226-236	11
β-strand	241-245	5	1
α-helix	246-250	5
α-helix	255-262	8
α-helix	264-280	17
α-helix	286-288	3
β-strand	294-298	5	1
β-strand	304	1	2
α-helix	306-308	3
α-helix	311-313	3
β-strand	317-327	11	2
β-strand	331-341	11	3
β-strand	348-352	5	3
α-helix	353	1
β-strand	373-385	13	3
β-strand	386-391	6	2
α-helix	398	1
α-helix	402-404	3
β-strand	405-410	6	2
β-strand	423-433	11	2
α-helix	436-442	7
β-strand	446	1	4
β-strand	448-458	11	2
α-helix	478-488	11
α-helix	493-499	7
α-helix	509-520	12
β-strand	525-527	3	5
β-strand	531-533	3	5
β-strand	539-543	5	6
α-helix	549-559	11
β-strand	563-567	5	6
α-helix	568-570	3
β-strand	571	1	7
β-strand	578-582	5	8
β-strand	589-593	5	8
α-helix	595-598	4
β-strand	602-607	6	6
α-helix	609-611	3
α-helix	614-617	4
α-helix	621-625	5
β-strand	628-633	6	9
β-strand	636-641	6	9
β-strand	644-650	7	6
α-helix	653-655	3
α-helix	663-666	4
α-helix	671-674	4
β-strand	679-683	5	6
β-strand	686	1	10
α-helix	691-705	15
α-helix	740-753	14
α-helix	760-773	14
β-strand	777-778	2	11
α-helix	783-796	14
α-helix	807-823	17
β-strand	828-829	2	11
α-helix	831-846	16
α-helix	851-860	10
α-helix	862-865	4

Chain 3: 36 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	19-34	16
α-helix	37-49	13
α-helix	50-55	6
β-strand	95-99	5	12
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-136	19
α-helix	144-147	4
β-strand	154-158	5	12
α-helix	162-164	3
β-strand	165-166	2	13
α-helix	173-175	3
β-strand	179-187	9	13
α-helix	189-192	4
β-strand	193-203	11	13
β-strand	208-213	6	13
α-helix	225-227	3
α-helix	230-232	3
β-strand	234	1	14
β-strand	240	1	14
β-strand	241-243	3	13
β-strand	249-259	11	13
α-helix	260-261	2
α-helix	262-264	3
α-helix	265-266	2
α-helix	269-272	4
β-strand	273-279	7	13
β-strand	291-301	11	13
β-strand	317	1	15
β-strand	318-329	12	13
α-helix	340-343	4
α-helix	344-353	10
α-helix	359-366	8
α-helix	375-385	11
β-strand	391	1	16
β-strand	399	1	16
β-strand	405-409	5	17
α-helix	415-425	11
β-strand	429-433	5	17
β-strand	437	1	18
α-helix	439-442	4
β-strand	444-449	6	19
β-strand	454-459	6	19
α-helix	460	1
α-helix	461-464	4
β-strand	469-473	5	17
α-helix	475-477	3
α-helix	480-492	13
β-strand	494-499	6	20
β-strand	502-507	6	20
β-strand	511-516	6	17
α-helix	529-532	4
α-helix	537-542	6
β-strand	545-549	5	17
α-helix	555-569	15
α-helix	576-577	2
α-helix	580-582	3
α-helix	653-665	13
α-helix	669	1
β-strand	670-671	2	21
α-helix	673-687	15
α-helix	699-715	17
β-strand	720-721	2	21
α-helix	723-738	16

Chain 4: 40 helices, 35 β-strands

Element	Residues	Length	Sheet
β-strand	178-180	3	22
β-strand	186	1	22
α-helix	187-198	12
β-strand	202	1	23
α-helix	203-205	3
α-helix	206-209	4
α-helix	218-221	4
β-strand	224	1	23
α-helix	225-236	12
β-strand	240-244	5	24
α-helix	246-250	5
α-helix	252-254	3
α-helix	255-263	9
α-helix	265-285	21
α-helix	294-297	4
β-strand	302-306	5	24
α-helix	314-316	3
α-helix	319-321	3
β-strand	325-335	11	25
α-helix	336-338	3
β-strand	339-349	11	25
β-strand	355-359	5	25
β-strand	360-361	2	26
β-strand	364-365	2	26
β-strand	370	1	27
β-strand	379	1	27
β-strand	382-384	3	25
α-helix	386-388	3
β-strand	390-401	12	25
α-helix	402	1
β-strand	414-420	7	25
α-helix	421-423	3
β-strand	432-442	11	25
β-strand	445	1	28
β-strand	453-454	2	28
β-strand	456	1	29
β-strand	458-468	11	25
α-helix	506-513	8
α-helix	518-523	6
α-helix	534-545	12
β-strand	564-567	4	30
α-helix	574-584	11
β-strand	589-592	4	30
α-helix	598-601	4
β-strand	603-605	3	31
β-strand	616-618	3	31
β-strand	628-632	5	30
α-helix	634-636	3
α-helix	639-642	4
α-helix	646-651	6
β-strand	653-658	6	32
β-strand	661-666	6	32
β-strand	670-675	6	30
α-helix	676-677	2
α-helix	681-683	3
α-helix	688-692	5
α-helix	696-699	4
β-strand	704-707	4	30
α-helix	714-724	11
α-helix	725-728	4
α-helix	746-757	12
β-strand	762	1	33
α-helix	764-779	16
α-helix	795-810	16
α-helix	811-813	3
β-strand	817	1	33
α-helix	819-833	15
α-helix	847-849	3
α-helix	852-875	24
β-strand	880-882	3	34
α-helix	883-889	7
α-helix	890-894	5
α-helix	900-912	13
β-strand	916-919	4	34
β-strand	923-926	4	34

Chain 5: 36 helices, 26 β-strands

Element	Residues	Length	Sheet
α-helix	21-37	17
β-strand	40	1	35
β-strand	43	1	35
α-helix	45-55	11
β-strand	60-64	5	36
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-100	18
α-helix	134-135	2
β-strand	136-140	5	36
α-helix	144-147	4
α-helix	148-150	3
α-helix	153-155	3
β-strand	159-169	11	15
α-helix	170-172	3
β-strand	173-183	11	15
β-strand	189-193	5	15
β-strand	241-259	19	15
α-helix	260-261	2
α-helix	262-264	3
α-helix	266	1
α-helix	269-272	4
β-strand	273-279	7	15
α-helix	280-282	3
β-strand	291-301	11	15
β-strand	323	1	3
β-strand	325-335	11	15
α-helix	351-360	10
α-helix	366-373	8
α-helix	382-393	12
β-strand	398-399	2	37
β-strand	405-406	2	37
β-strand	412-416	5	38
α-helix	422-432	11
β-strand	436-440	5	38
α-helix	441-443	3
β-strand	444	1	9
α-helix	446-449	4
β-strand	451-455	5	39
β-strand	462-466	5	39
α-helix	468-471	4
β-strand	476-480	5	38
α-helix	482-484	3
α-helix	487-490	4
α-helix	493-496	4
β-strand	501-506	6	18
β-strand	509-514	6	18
β-strand	518-523	6	38
α-helix	544-547	4
β-strand	552-556	5	38
α-helix	562-576	15
α-helix	579-590	12
α-helix	596-609	14
α-helix	612	1
β-strand	613-614	2	40
α-helix	615	1
α-helix	616-640	25
α-helix	650-666	17
β-strand	671-672	2	40
α-helix	674-684	11
α-helix	685-689	5
α-helix	690-693	4

Chain 6: 34 helices, 34 β-strands

Element	Residues	Length	Sheet
β-strand	94-97	4	41
α-helix	99-101	3
α-helix	102-104	3
α-helix	106-121	16
β-strand	124-125	2	42
β-strand	131-132	2	42
α-helix	135-146	12
β-strand	150-154	5	43
α-helix	155-160	6
α-helix	161-164	4
α-helix	165-172	8
α-helix	174-192	19
β-strand	198	1	44
α-helix	259	1
β-strand	260	1	44
α-helix	261-262	2
β-strand	264-268	5	43
β-strand	274-275	2	4
α-helix	276-278	3
α-helix	281-283	3
β-strand	287-297	11	4
β-strand	301-311	11	4
β-strand	317-322	6	4
β-strand	344-346	3	4
β-strand	352-362	11	4
α-helix	373-375	3
β-strand	376-382	7	4
α-helix	383-385	3
β-strand	394-405	12	4
α-helix	407-410	4
β-strand	437	1	45
β-strand	438	1	26
β-strand	448	1	45
β-strand	450-461	12	4
α-helix	501-507	7
α-helix	510-520	11
α-helix	525-532	8
α-helix	541-552	12
α-helix	554-556	3
β-strand	557-558	2	46
β-strand	564-565	2	46
β-strand	571-575	5	47
α-helix	581-591	11
β-strand	596-599	4	47
α-helix	605-608	4
β-strand	610-614	5	48
β-strand	621-625	5	48
α-helix	627-630	4
β-strand	635-639	5	47
α-helix	641-643	3
α-helix	646-649	4
α-helix	650-652	3
α-helix	653-657	5
β-strand	660-665	6	7
β-strand	668-673	6	7
β-strand	677-682	6	47
α-helix	695-698	4
α-helix	703-706	4
β-strand	711-715	5	47
α-helix	721-734	14
α-helix	748-758	11
β-strand	764-765	2	49
α-helix	767-783	17
β-strand	786	1	10
β-strand	791	1	10
α-helix	797-813	17
β-strand	818-819	2	49
α-helix	821-835	15

Chain 7: 34 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	7-8	2	13
α-helix	14-27	14
α-helix	31-33	3
α-helix	62-71	10
β-strand	77-81	5	50
α-helix	82-94	13
α-helix	102-106	5
α-helix	110-122	13
α-helix	125-128	4
α-helix	134-136	3
α-helix	139-154	16
α-helix	194-197	4
β-strand	201-205	5	50
α-helix	224	1
β-strand	225	1	29
α-helix	226	1
α-helix	227-229	3
α-helix	232-234	3
β-strand	238-248	11	29
α-helix	249-251	3
β-strand	252-262	11	29
β-strand	268-272	5	29
β-strand	277-278	2	28
β-strand	283	1	51
α-helix	287-291	5
β-strand	297	1	51
β-strand	298-300	3	29
α-helix	302-304	3
β-strand	306-316	11	29
β-strand	330-336	7	29
α-helix	337-339	3
β-strand	348-358	11	29
α-helix	363-367	5
β-strand	371	1	13
β-strand	373-383	11	29
α-helix	397-405	9
α-helix	410-416	7
α-helix	426-437	12
β-strand	442	1	52
β-strand	450	1	52
β-strand	456-459	4	53
α-helix	466-476	11
β-strand	481-484	4	53
β-strand	496-499	4	54
β-strand	506-509	4	54
α-helix	512-515	4
β-strand	520-524	5	53
α-helix	531-543	13
β-strand	545-547	3	55
β-strand	550	1	56
β-strand	553	1	56
β-strand	556-558	3	55
β-strand	562-566	5	53
α-helix	580-583	4
α-helix	588-591	4
β-strand	596-599	4	53
α-helix	606-621	16
α-helix	625-627	3
α-helix	631-634	4
α-helix	635-646	12
α-helix	654-671	18
α-helix	686-702	17
α-helix	713-728	16

Chain A: 7 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	3-22	20
α-helix	27-32	6
α-helix	34-60	27
α-helix	64-103	40
α-helix	120-122	3
α-helix	125-142	18
β-strand	162-167	6	57
β-strand	172-176	5	58
β-strand	179-183	5	58
β-strand	188-192	5	57
α-helix	193-201	9
β-strand	205-206	2	57

Chain B: 10 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	5-7	3
α-helix	13-20	8
β-strand	24-29	6	59
α-helix	34-36	3
α-helix	57-59	3
β-strand	69-72	4	59
α-helix	73-81	9
β-strand	85-87	3	59
α-helix	95-107	13
α-helix	119-129	11
α-helix	138-159	22
α-helix	160-162	3
β-strand	168-169	2	60
α-helix	175-199	25

8 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2	protein	868	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P29469 (AlphaFold model)
DNA replication licensing factor MCM3	3	protein	971	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P24279 (AlphaFold model)
DNA replication licensing factor MCM4	4	protein	933	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P30665 (AlphaFold model)
Minichromosome maintenance protein 5	5	protein	775	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P29496 (AlphaFold model)
DNA replication licensing factor MCM6	6	protein	1017	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P53091
DNA replication licensing factor MCM7	7	protein	845	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P38132
DNA replication complex GINS protein PSF1	A	protein	208	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q12488
DNA replication complex GINS protein PSF2	B	protein	213	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P40359
DNA replication complex GINS protein PSF3	C	protein	217	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q12146
DNA replication complex GINS protein SLD5	D	protein	294	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q03406
Cell division control protein 45	E	protein	650	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q08032
DNA polymerase alpha-binding protein	F, G, H	protein	927	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q01454

4 more molecules are not listed.

Sequence of entity 1 (2), FASTA

>6SKL_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 2 (3), FASTA

>6SKL_2 DNA replication licensing factor MCM3 (chains 3)
MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQ
DDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAY
FIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLV
SVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNK
LTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKS
LGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIF
DILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLR
FVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTD
VDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLL
SRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINP
EEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYV
QYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKT
VNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSG
SPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQL
GLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTIST
GRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNN
LMVADDKVWRV

Sequence of entity 3 (4), FASTA

>6SKL_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 4 (5), FASTA

>6SKL_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 5 (6), FASTA

>6SKL_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Sequence of entity 6 (7), FASTA

>6SKL_6 DNA replication licensing factor MCM7 (chains 7)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA

Sequence of entity 7 (A), FASTA

>6SKL_7 DNA replication complex GINS protein PSF1 (chains A)
MYGDLGNKLVLEAKRTKQLYARSNQDVNLPMYHEDIIRNILKEVSNLRKNTEYLKEQQQL
GMLDDKVAKCQYFVTLLCMERNKRCLLAYQRLRTDILDSMAWNNNGLDLMSSITFSQQDT
NNLSHQEQEYLKEYCDLITDLKSGDLVDIDLSGSLVPPSDVFIDVRVLKDAGEIQTEYGV
FNLIKDSQFFVRQSDVERLIQQGYLQKI

Sequence of entity 8 (B), FASTA

>6SKL_8 DNA replication complex GINS protein PSF2 (chains B)
MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGTGNHTRWQLITTDDKAL
NNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKIQYEKTHPDRFSELPWNWL
VLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGLKYLNESHLQLDNLSLLEINE
LRPFITEIMDKLREIHTASLTAGTENDEEEFNI

Sequence of entity 9 (C), FASTA

>6SKL_9 DNA replication complex GINS protein PSF3 (chains C)
MGSSHHHHHHSSGLVPRGSHMASMGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRP
ITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDL
HSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSAN
TNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK

Sequence of entity 10 (D), FASTA

>6SKL_10 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI

Sequence of entity 11 (E), FASTA

>6SKL_11 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEY
YSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVK
RLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLH
KMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSIS
ASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLS
WDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYR
NPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKP
ILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL

Sequence of entity 12 (F, G, H), FASTA

>6SKL_12 DNA polymerase alpha-binding protein (chains F, G, H)
MVSVIDKLVFDFGGKTLVSLAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSI
KCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELIL
LELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYI
VANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENNVADPEFCAANRICTRVAWHPK
GLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKL
TVWNWETSEIHYTREFKRKITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIP
DQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSGL
SEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFS
PAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLN
EKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSF
NQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMS
LPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWLP
ILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPV
FVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDT
LENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVK
ISERAELPSLVKKINNIREARYEQQLK

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	5
ANP	Phosphoaminophosphonic acid-adenylate ester	C10 H17 N6 O12 P3	3
MG	Magnesium ion	Mg	3

Primary citation

Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork. Baretic, D., Jenkyn-Bedford, M., Aria, V. et al. Mol Cell (2020) 78:926-940.e13. DOI 10.1016/j.molcel.2020.04.012 · PubMed

Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
8KG6 3.07 Å, Yeast replisome in state I
9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD

Browse structure collections

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