6UCV: Mitochondrial TOM complex from yeast
Cryo-EM structure of the mitochondrial TOM complex from yeast (tetramer). Determined by electron microscopy at 4.1 Å resolution. Released 6 Nov 2019.
- Method
- Electron microscopy
- Resolution
- 4.1 Å
- Organism
- Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
- Chains
- 20
- Atoms
- 15,102
- Mol. weight
- 323.47 kDa
- Ligands
- MC3
- Released
- 6 Nov 2019
Explore 6UCV in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
6UCV contains 44 α-helices and 76 β-strands across 20 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain a: 4 helices, 19 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 40-53 | 14 | |
| α-helix | 68-74 | 7 | |
| β-strand | 85-93 | 9 | 3 |
| β-strand | 97-105 | 9 | 3 |
| β-strand | 114-121 | 8 | 3 |
| β-strand | 125-132 | 8 | 3 |
| β-strand | 137-144 | 8 | 3 |
| β-strand | 150-157 | 8 | 3 |
| β-strand | 164-172 | 9 | 3 |
| β-strand | 176-187 | 12 | 3 |
| β-strand | 193-203 | 11 | 3 |
| β-strand | 209-219 | 11 | 3 |
| α-helix | 225-226 | 2 | |
| β-strand | 227-237 | 11 | 3 |
| β-strand | 243-249 | 7 | 3 |
| β-strand | 255-261 | 7 | 3 |
| β-strand | 267-275 | 9 | 3 |
| β-strand | 299-308 | 10 | 3 |
| β-strand | 312-319 | 8 | 3 |
| β-strand | 323-330 | 8 | 3 |
| β-strand | 336-345 | 10 | 3 |
| β-strand | 350-362 | 13 | 3 |
| α-helix | 365-372 | 8 | |
Chain A: 4 helices, 19 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 44-54 | 11 | |
| α-helix | 68-74 | 7 | |
| β-strand | 83-93 | 11 | 1 |
| β-strand | 97-106 | 10 | 1 |
| β-strand | 114-121 | 8 | 1 |
| β-strand | 125-132 | 8 | 1 |
| β-strand | 137-144 | 8 | 1 |
| β-strand | 149-157 | 9 | 1 |
| β-strand | 164-172 | 9 | 1 |
| β-strand | 176-187 | 12 | 1 |
| β-strand | 193-203 | 11 | 1 |
| β-strand | 209-219 | 11 | 1 |
| α-helix | 225-226 | 2 | |
| β-strand | 227-237 | 11 | 1 |
| β-strand | 243-249 | 7 | 1 |
| β-strand | 255-262 | 8 | 1 |
| β-strand | 267-275 | 9 | 1 |
| β-strand | 299-308 | 10 | 1 |
| β-strand | 312-319 | 8 | 1 |
| β-strand | 323-330 | 8 | 1 |
| β-strand | 336-345 | 10 | 1 |
| β-strand | 350-362 | 13 | 1 |
| α-helix | 365-372 | 8 | |
Chain b: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 85-134 | 50 | |
Chains B and j: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 87-134 | 48 | |
Chains c, C, k and K: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-48 | 35 | |
Chain d: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 30-50 | 21 | |
| α-helix | 52-57 | 6 | |
Chains D and L: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 31-50 | 20 | |
| α-helix | 52-57 | 6 | |
Chains e, E, m and M: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 12-30 | 19 | |
| α-helix | 32-42 | 11 | |
| α-helix | 50-54 | 5 | |
4 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Mitochondrial import receptor subunit TOM40 | A, I, a, i | protein | 397 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P23644 (AlphaFold model) |
| Mitochondrial import receptor subunit TOM22 | B, J, b, j | protein | 162 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P49334 (AlphaFold model) |
| Mitochondrial import receptor subunit TOM5 | C, K, c, k | protein | 50 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P80967 (AlphaFold model) |
| Mitochondrial import receptor subunit TOM6 | D, L, d, l | protein | 61 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P33448 (AlphaFold model) |
| Mitochondrial import receptor subunit TOM7 | E, M, e, m | protein | 60 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P53507 |
Sequence of entity 1 (A, I, a, i), FASTA
>6UCV_1 Mitochondrial import receptor subunit TOM40 (chains A, I, a, i)
MSAPTPLAEASQIPTIPALSPLTAKQSKGNFFSSNPISSFVVDTYKQLHSHRQSLELVNP
GTVENLNKEVSRDVFLSQYFFTGLRADLNKAFSMNPAFQTSHTFSIGSQALPKYAFSALF
ANDNLFAQGNIDNDLSVSGRLNYGWDKKNISKVNLQISDGQPTMCQLEQDYQASDFSVNV
KTLNPSFSEKGEFTGVAVASFLQSVTPQLALGLETLYSRTDGSAPGDAGVSYLTRYVSKK
QDWIFSGQLQANGALIASLWRKVAQNVEAGIETTLQAGMVPITDPLMGTPIGIQPTVEGS
TTIGAKYEYRQSVYRGTLDSNGKVACFLERKVLPTLSVLFCGEIDHFKNDTKIGCGLQFE
TAGNQELLMLQQGLDADGNPLQALPQLGGWSHPQFEK
Sequence of entity 2 (B, J, b, j), FASTA
>6UCV_2 Mitochondrial import receptor subunit TOM22 (chains B, J, b, j)
MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDSDSDFEDEFDENETLLD
RIVALKDIVPPGKRQTISNFFGFTSSFVRNAFTKSGNLAWTLTTTALLLGVPLSLSILAE
QQLIEMEKTFDLQSDANNILAQGEKDAAATANGGHHHHHHHH
Sequence of entity 3 (C, K, c, k), FASTA
>6UCV_3 Mitochondrial import receptor subunit TOM5 (chains C, K, c, k)
MFGLPQQEVSEEEKRAHQEQTEKTLKQAAYVAAFLWVSPMIWHLVKKQWK
Sequence of entity 4 (D, L, d, l), FASTA
>6UCV_4 Mitochondrial import receptor subunit TOM6 (chains D, L, d, l)
MDGMFAMPGAAAGAASPQQPKSRFQAFKESPLYTIALNGAFFVAGVAFIQSPLMDMLAPQ
L
Sequence of entity 5 (E, M, e, m), FASTA
>6UCV_5 Mitochondrial import receptor subunit TOM7 (chains E, M, e, m)
MSFLPSFILSDESKERISKILTLTHNVAHYGWIPFVLYLGWAHTSNRPNFLNLLSPLPSV
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| MC3 | 1,2-dimyristoyl-rac-glycero-3-phosphocholine | C36 H72 N O8 P | 2 |
Primary citation
Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution. Tucker, K., Park, E. Nat Struct Mol Biol (2019) 26:1158-1166. DOI 10.1038/s41594-019-0339-2 · PubMed
Other PDB entries of the same protein (UniProt P23644 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 9J99 2.94 Å, Substrate-engaged TOM complex from yeast
- 7E4H 3.01 Å, Cryo-EM structure of the yeast mitochondrial SAM-Tom40 complex at 3.0 angstrom
- 7E4I 3.05 Å, Cryo-EM structure of the yeast mitochondrial SAM-Tom40/Tom5/Tom6 complex at 3.0 angstrom
- 6UCU 3.06 Å, Cryo-EM structure of the mitochondrial TOM complex from yeast (dimer)
- 7VKU 3.2 Å, Cryo-EM structure of SAM-Tom40 intermediate complex
- 8XKY 3.42 Å, Structure of the TOM40 complex annealed
- 8W5K 3.6 Å, Cryo-EM structure of the yeast TOM core complex crosslinked by BS3 (from TOM-TIM23…
- 8XKW 3.64 Å, Structure of the TOM40 complex unannealed
- 8XKX 3.7 Å, Structure of the TOM40 complex with pre-protein
- 6JNF 3.81 Å, Cryo-EM structure of the translocator of the outer mitochondrial membrane
- 8HCO 4.1 Å, Substrate-engaged TOM complex from yeast
- 8W5J 4.4 Å, Cryo-EM structure of the yeast TOM core complex (from TOM-TIM23 complex)
Browse structure collections
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