7APX: Yeast THO-Sub2 complex

yeast THO-Sub2 complex. Determined by electron microscopy at 3.4 Å resolution. Released 2 Dec 2020.

Method
Electron microscopy
Resolution
3.4 Å
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Chains
6
Atoms
20,552
Mol. weight
433.84 kDa
Released
2 Dec 2020

Explore 7APX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7APX contains 141 α-helices and 57 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 74 helices, 12 β-strands

ElementResiduesLengthSheet
α-helix37-5014
α-helix54-7118
α-helix86-10015
α-helix106-1149
α-helix124-1318
α-helix136-1427
α-helix152-16514
β-strand17211
α-helix179-19315
α-helix198-2014
α-helix202-21514
β-strand21811
α-helix220-23213
α-helix235-24814
α-helix263-2653
β-strand27012
α-helix272-28110
α-helix290-2923
α-helix294-30411
β-strand31012
α-helix313-3164
α-helix323-34220
α-helix395-4017
α-helix404-41310
α-helix417-4248
α-helix428-4325
α-helix436-45520
α-helix468-4703
β-strand471-47553
β-strand478-48253
β-strand484-48524
β-strand487-49155
β-strand497-49825
β-strand502-50655
α-helix511-5133
α-helix521-53515
α-helix536-5416
α-helix543-55917
α-helix566-57611
α-helix577-5815
α-helix582-5854
α-helix592-60211
α-helix607-61913
α-helix626-64318
α-helix651-66414
α-helix666-6694
α-helix671-6799
α-helix683-6908
α-helix691-6933
α-helix696-71116
β-strand71716
α-helix7231
β-strand72416
α-helix7251
α-helix728-74114
α-helix748-75710
α-helix758-7603
α-helix763-77210
α-helix773-7775
α-helix787-7926
α-helix797-80610
α-helix815-82612
α-helix827-8293
α-helix831-84616
α-helix852-87625
α-helix879-8846
α-helix889-8946
α-helix900-91617
α-helix942-9509
α-helix961-9699
α-helix984-9929
α-helix994-101724
α-helix1025-10306
α-helix1031-10355
α-helix1036-10394
α-helix1043-105311
α-helix1059-107012
α-helix1075-10817
α-helix1084-110623
α-helix1114-113219
α-helix1138-115114
α-helix1152-11543
α-helix1159-116911
α-helix1177-118610
α-helix1206-122116
α-helix3002-301817
Chain B: 33 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix3-2119
α-helix26-316
α-helix33-353
α-helix36-383
α-helix44-463
α-helix56-583
α-helix62-7716
α-helix92-10514
α-helix113-1153
α-helix116-13015
α-helix134-14613
α-helix148-1514
α-helix159-1613
α-helix174-19118
α-helix197-21317
α-helix251-26212
α-helix263-2686
α-helix272-2743
α-helix280-31940
α-helix337-3404
α-helix344-36017
α-helix368-3703
α-helix379-3868
α-helix393-41523
α-helix418-42912
α-helix443-45210
α-helix453-4619
α-helix462-4632
α-helix471-49020
α-helix494-4963
α-helix506-5094
α-helix526-5316
α-helix577-58610
Chain C: 6 helices, 1 β-strand
ElementResiduesLengthSheet
α-helix9-3729
α-helix47-8236
α-helix87-915
α-helix97-12226
β-strand141-14224
α-helix151-1588
α-helix167-23468
Chain D: 7 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix5-1511
α-helix22-4322
α-helix61-10141
α-helix107-14236
α-helix154-1585
α-helix161-1688
α-helix199-24648
Chain E: 3 helices, 28 β-strands
ElementResiduesLengthSheet
α-helix23-319
β-strand3917
α-helix43-5210
β-strand66-7168
β-strand78-8258
β-strand87-9158
β-strand101-10228
β-strand117-11939
β-strand127-13159
β-strand136-14279
β-strand149-15689
β-strand163-168610
β-strand174-179610
β-strand184-188510
β-strand194-199610
α-helix201-2055
β-strand214-217411
β-strand223-227511
β-strand233-237511
β-strand244-249611
β-strand256-261612
β-strand267-272612
β-strand277-281512
β-strand287-291512
β-strand298-303613
β-strand309-314613
β-strand319-323513
β-strand329-332413
β-strand344-34637
β-strand353-35647
β-strand362-36327
Chain F: 18 helices, 16 β-strands
ElementResiduesLengthSheet
α-helix63-675
α-helix71-788
α-helix87-9610
β-strand102-104314
α-helix112-12312
β-strand133-136414
α-helix140-15314
β-strand162-165414
α-helix171-1799
β-strand187-190414
α-helix192-2009
β-strand211-215514
α-helix217-2226
α-helix224-23512
β-strand242-246514
β-strand281-283315
β-strand284-285216
β-strand286-287217
α-helix290-2923
α-helix293-30311
β-strand308-312515
α-helix316-32813
β-strand333-336415
α-helix342-35312
β-strand359-362415
α-helix364-3663
β-strand375-380615
α-helix387-3948
β-strand404-408515
β-strand409-410217
α-helix413-42614
β-strand431-432216
α-helix433-4342
α-helix440-4423

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
THO complex subunit 2,Tho2Aprotein1620Saccharomyces cerevisiae (strain ATCC 204508 / S288c)P53552 (AlphaFold model)
THO complex subunit HPR1Bprotein720Saccharomyces cerevisiae (strain ATCC 204508 / S288c)P17629 (AlphaFold model)
THO complex subunit THP2Cprotein261Saccharomyces cerevisiae (strain ATCC 204508 / S288c)O13539 (AlphaFold model)
THO complex subunit MFT1Dprotein392Saccharomyces cerevisiae (strain ATCC 204508 / S288c)P33441 (AlphaFold model)
Protein TEX1Eprotein380Saccharomyces cerevisiae (strain ATCC 204508 / S288c)P53851
ATP-dependent RNA helicase SUB2Fprotein400Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Q07478
Sequence of entity 1 (A), FASTA
>7APX_1 THO complex subunit 2,Tho2 (chains A)
GAASMAEQTLLSKLNALSQKVIPPASPSQASILTEEVIRNWPERSKTLCSDFTALESNDE
KEDWLRTLFIELFDFINKNDENSPLKLSDVASFTNELVNHERQVSQASIVGKMFIAVSST
VPNINDLTTISLCKLIPSLHEELFKFSWISSKLLNKEQTTLLRHLLKKSKYELKKYNLLV
ENSVGYGQLVALLILAYYDPDNFSKVSAYLKEIYHIMGKYSLDSIRTLDVILNVSSQFIT
EGYKFFIALLRKSDSWPSSHVANNSNYSSLNEGGNMIAANIISFNLSQYNEEVDKENYER
YMDMCCILLKNGFVNFYSIWDNVKPEMEFLQEYIQNLETELEEESTKGVENPLAMAAALS
TENETDEDNALVVNDDVNMKDKISEETNADIESKGKQKTQQDILLFGKIKLLERLLIHGC
VIPVIHVLKQYPKVLYVSESLSRYLGRVFEYLLNPLYTSMTSSGESKDMATALMITRIDN
GILAHKPRLIHKYKTHEPFESLELNSSYVFYYSEWNSNLTPFASVNDLFENSHIYLSIIG
PYLGRIPTLLSKISRIGVADIQKNHGSESLHVTIDKWIDYVRKFIFPATSLLQNNPIATS
EVYELMKFFPFEKRYFIYNEMMTKLSQDILPLKVSFNKAEREAKSILKALSIDTIAKESR
RFAKLISTNPLASLVPAVKQIENYDKVSELVVYTTKYFNDFAYDVLQFVLLLRLTYNRPA
VQFDGVNQAMWVQRLSIFIAGLAKNCPNMDISNIITYILKTLHNGNIIAVSILKELIITV
GGIRDLNEVNMKQLLMLNSGSPLKQYARHLIYDFRDDNSVISSRLTSFFTDQSAISEIIL
LLYTLNLKANTQNSHYKILSTRCDEMNTLLWSFIELIKHCLKGKAFEENVLPFVELNNRF
HLSTPWTFHIWRDYLDNQLNSNENFSIDELIEGAEFSDVDLTKISKDLFTTFWRLSLYDI
HFDKSLYDERKNALSGENTGHMSNRKKHLIQNQIKDILVTGISHQRAFKKTSEFISEKSN
VWNKDCGEDQIKIFLQNCVVPRVLFSPSDALFSSFFIFMAFRTENLMSILNTCITSNILK
TLLFCCTSSEAGNLGLFFTDVLKKLEKMRLNGDFNDQASRKLYEWHSVITEQVIDLLSEK
NYMSIRNGIEFMKHVTSVFPVVKAHIQLVYTTLEENLINEEREDIKLPSSALIGHLKARL
KDALELDEFCTLTEEEAEQKRIREMELEEIKNYETACQNEQKQVALRKQLELNKSQRLQN
DPPKSVASGSAGLNSKDRYTYSRNEPVIPTKPSSSQWSYSKVTRHVDDINHYLATNHLQK
AISLVENDDETRNLRKLSKQNMPIFDFRNSTLEIFERYFRTLIQNPQNPDFAEKIDSLKR
YIKNISREPYPDTTSSYSEAAAPEYTKRSSRYSGNAGGKDGYGSSNYRGPSNDRSAPKNI
KPISSYAHKRSELPTRPSKSKTYNDRSRALRPTGPDRGDGFDQRDNRLREEYKKNSSQRS
QLRFPEKPFQEGKDSSKANPYQASSYKRDSPSENEEKPNKRFKKDETIRNKFQTQDYRNT
RDSGAAHRANENQRYNGNRKSNTQALPQGPKGGNYVSRYQRXXXXXXXXXXXXXXXXXXX
Sequence of entity 2 (B), FASTA
>7APX_2 THO complex subunit HPR1 (chains B)
MSNTEELIQNSIGFLQKTFKALPVSFDSIRHEPLPSSMLHASVLNFEWEPLEKNISAIHD
RDSLIDIILKRFIIDSMTNAIEDEEENNLEKGLLNSCIGLDFVYNSRFNRSNPASWGNTF
FELFSTIIDLLNSPSTFLKFWPYAESRIEWFKMNTSVEPVSLGESNLISYKQPLYEKLRH
WNDILAKLENNDILNTVKHYNMKYKLENFLSELLPINEESNFNRSASISALQESDNEWNR
SARERESNRSSDVIFAADYNFVFYHLIICPIEFAFSDLEYKNDVDRSLSPLLDAILEIEE
NFYSKIKMNNRTRYSLEEALNTEYYANYDVMTPKLPVYMKHSNAMKMDRNEFWANLQNIK
ESDDYTLRPTIMDISLSNTTCLYKQLTQEDDDYYRKQFILQLCFTTNLIRNLISSDETRN
FYKSCYLRENPLSDIDFENLDEVNKKRGLNLCSYICDNRVLKFYKIKDPDFYRVIRKLMS
SDEKFTTAKIDGFKEFQNFRISKEKIPPPAFDETFKKFTFIKMGNKLINNVWKIPTGLDK
IEQEVKKPEGVYEAAQAKWESKISSETSGGEAKDEIIRQWQTLRFLRSRYLFDFDKVNEK
TGVDGLFEEPRKVEALDDSFKEKLLYKINQEHRKKLQDAREYKIGKERKKRALEEEASFP
EREQKIKSQRINSASQTEGDELKSEQTQPKGEISEENTKIKSSEVSSQDPDSGVAGEFAP
Sequence of entity 3 (C), FASTA
>7APX_3 THO complex subunit THP2 (chains C)
MTKEEGRTYFESLCEEEQSLQESQTHLLNILDILSVLADPRSSDDLLTESLKKLPDLHRE
LINSSIRLRYDKYQTREAQLLEDTKTGRDVAAGVQNPKSISEYYSTFEHLNRDTLRYINL
LKRLSVDLAKQVEVSDPSVTVYEMDKWVPSEKLQGILEQYCAPDTDIRGVDAQIKNYLDQ
IKMARAKFGLENKYSLKERLSTLTKELNHWRKEWDDIEMLMFGDDAHSMKKMIQKIDSLK
SEINAPSESYPVDKEGDIVLE
Sequence of entity 4 (D), FASTA
>7APX_4 THO complex subunit MFT1 (chains D)
MPLSQKQIDQVRTKVHYSEVDTPFNKYLDILGKVTKLTGSIINGTLSNDDSKIEKLTEQN
ISQLKESAHLRFLDLQSSIDTKKVADENWETCQQETLAKLENLKDKLPDIKSIHSKLLLR
IGKLQGLYDSVQVINREVEGLSEGRTSLVVTRAEWEKELGTDLVKFLIEKNYLKLVDPGL
KKDSSEERYRIYDDFSKGPKELESINASMKSDIENVRQEVSSYKEKWLRDAEIFGKITSI
FKEELLKRDGLLNEAEGDNIDEDYESDEDEERKERFKRQRSMVEVNTIENVDEKEESDHE
YDDQEDEENEEEDDMEVDVEDIKEDNEVDGESSQQEDNSRQGNNEETDKETGVIEEPDAV
NDAEEADSDHSSRKLGGTTSDFSASSSVEEVK
Sequence of entity 5 (E), FASTA
>7APX_5 Protein TEX1 (chains E)
MSTIGAVDILNQKTITSEVAASVTSKYLQSTFSKGNTSHIEDKRFIHVSSRSHSRFTSTP
ITPNEILSLKFHVSGSSMAYSRMDGSLTVWFIKDASFDKSVEVYIPDCCGSDKLATDLSW
NPTSLNQIAVVSNSSEISLLLINEKSLTASKLRTLSLGSKTKVNTCLYDPLGNWLLAATK
SEKIYLFDVKKDHSSVCSLNISDISQEDNDVVYSLAWSNGGSHIFIGFKSGYLAILKAKH
GILEVCTKIKAHTGPITEIKMDPWGRNFITGSIDGNCYVWNMKSLCCELIINDLNSAVTT
LDVCHLGKILGICTEDEMVYFYDLNSGNLLHSKSLANYKTDPVLKFYPDKSWYIMSGKND
TLSNHFVKNEKNLITYWKDM
Sequence of entity 6 (F), FASTA
>7APX_6 ATP-dependent RNA helicase SUB2 (chains F)
GAASDKKGSYVGIHSTGFKDFLLKPELSRAIIDCGFEHPSEVQQHTIPQSIHGTDVLCQA
KSGLGKTAVFVLSTLQQLDPVPGEVAVVVICNARELAYQIRNEYLRFSKYMPDVKTAVFY
GGTPISKDAELLKNKDTAPHIVVATPGRLKALVREKYIDLSHVKNFVIDECDKVLEELDM
RRDVQEIFRATPRDKQVMMFSATLSQEIRPICRRFLQNPLEIFVDDEAKLTLHGLQQYYI
KLEEREKNRKLAQLLDDLEFNQVIIFVKSTTRANELTKLLNASNFPAITVHGHMKQEERI
ARYKAFKDFEKRICVSTDVFGRGIDIERINLAINYDLTNEADQYLHRVGRAGRFGTKGLA
ISFVSSKEDEEVLAKIQERFDVKIAEFPEEGIDPSTYLNN

Primary citation

Structural insights into the nucleic acid remodeling mechanisms of the yeast THO-Sub2 complex. Schuller, S.K., Schuller, J.M., Prabu, J.R. et al. Elife (2020) 9. DOI 10.7554/eLife.61467 · PubMed

Other PDB entries of the same protein (UniProt P53552 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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