7LUV: Yeast THO-Sub2 complex
Cryo-EM structure of the yeast THO-Sub2 complex. Determined by electron microscopy at 3.7 Å resolution. Released 14 Apr 2021.
- Method
- Electron microscopy
- Resolution
- 3.7 Å
- Organisms
- Saccharomyces cerevisiae, Saccharomyces bayanus
- Chains
- 6
- Atoms
- 18,773
- Mol. weight
- 361.44 kDa
- Released
- 14 Apr 2021
Explore 7LUV in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7LUV contains 124 α-helices and 52 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 26 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-18 | 13 | |
| α-helix | 27-30 | 4 | |
| α-helix | 41-43 | 3 | |
| α-helix | 62-76 | 15 | |
| α-helix | 90-105 | 16 | |
| α-helix | 117-130 | 14 | |
| α-helix | 134-146 | 13 | |
| α-helix | 148-151 | 4 | |
| α-helix | 159-161 | 3 | |
| α-helix | 174-190 | 17 | |
| α-helix | 197-213 | 17 | |
| α-helix | 253-262 | 10 | |
| α-helix | 263-267 | 5 | |
| α-helix | 271-274 | 4 | |
| α-helix | 280-320 | 41 | |
| α-helix | 337-341 | 5 | |
| α-helix | 344-361 | 18 | |
| α-helix | 368-370 | 3 | |
| α-helix | 381-386 | 6 | |
| α-helix | 393-414 | 22 | |
| α-helix | 416-429 | 14 | |
| α-helix | 444-453 | 10 | |
| α-helix | 461-467 | 7 | |
| α-helix | 469-490 | 22 | |
| α-helix | 495-497 | 3 | |
| α-helix | 526-532 | 7 | |
Chain B: 9 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-37 | 29 | |
| α-helix | 54-82 | 29 | |
| α-helix | 103-121 | 19 | |
| β-strand | 141 | 1 | 1 |
| α-helix | 151-158 | 8 | |
| α-helix | 168-191 | 24 | |
| α-helix | 192-196 | 5 | |
| α-helix | 197-205 | 9 | |
| α-helix | 208-210 | 3 | |
| α-helix | 213-224 | 12 | |
Chain C: 64 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 41-49 | 9 | |
| α-helix | 58-72 | 15 | |
| α-helix | 84-95 | 12 | |
| α-helix | 106-115 | 10 | |
| α-helix | 125-129 | 5 | |
| α-helix | 136-142 | 7 | |
| α-helix | 144-149 | 6 | |
| α-helix | 152-166 | 15 | |
| α-helix | 181-193 | 13 | |
| α-helix | 198-200 | 3 | |
| α-helix | 202-215 | 14 | |
| α-helix | 220-231 | 12 | |
| α-helix | 235-238 | 4 | |
| α-helix | 242-248 | 7 | |
| α-helix | 263-265 | 3 | |
| α-helix | 272-282 | 11 | |
| α-helix | 294-306 | 13 | |
| α-helix | 313-316 | 4 | |
| α-helix | 323-339 | 17 | |
| α-helix | 399-402 | 4 | |
| α-helix | 405-413 | 9 | |
| α-helix | 418-426 | 9 | |
| α-helix | 428-432 | 5 | |
| α-helix | 436-455 | 20 | |
| α-helix | 468-470 | 3 | |
| β-strand | 471-474 | 4 | 2 |
| β-strand | 479-482 | 4 | 2 |
| β-strand | 484 | 1 | 1 |
| β-strand | 488-491 | 4 | 3 |
| β-strand | 497-498 | 2 | 3 |
| β-strand | 503-506 | 4 | 3 |
| α-helix | 522-526 | 5 | |
| α-helix | 537-541 | 5 | |
| α-helix | 543-559 | 17 | |
| α-helix | 565-571 | 7 | |
| α-helix | 573-576 | 4 | |
| α-helix | 577-581 | 5 | |
| α-helix | 582-587 | 6 | |
| α-helix | 592-596 | 5 | |
| α-helix | 599-602 | 4 | |
| α-helix | 610-619 | 10 | |
| α-helix | 626-642 | 17 | |
| α-helix | 651-662 | 12 | |
| α-helix | 666-669 | 4 | |
| α-helix | 671-680 | 10 | |
| α-helix | 683-690 | 8 | |
| α-helix | 691-693 | 3 | |
| α-helix | 696-709 | 14 | |
| α-helix | 728-741 | 14 | |
| α-helix | 751-758 | 8 | |
| α-helix | 763-772 | 10 | |
| α-helix | 773-777 | 5 | |
| α-helix | 787-793 | 7 | |
| α-helix | 797-807 | 11 | |
| α-helix | 815-826 | 12 | |
| α-helix | 832-840 | 9 | |
| α-helix | 857-876 | 20 | |
| α-helix | 881-885 | 5 | |
| α-helix | 901-907 | 7 | |
| α-helix | 6942-6948 | 7 | |
| α-helix | 6992-7011 | 20 | |
| α-helix | 7029-7033 | 5 | |
| α-helix | 7051-7057 | 7 | |
| α-helix | 7132-7140 | 9 | |
| α-helix | 7143-7148 | 6 | |
| α-helix | 7160-7174 | 15 | |
| α-helix | 7181-7199 | 19 | |
| α-helix | 7212-7222 | 11 | |
| α-helix | 7235-7245 | 11 | |
| α-helix | 7257-7265 | 9 | |
Chain D: 5 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-16 | 11 | |
| α-helix | 22-38 | 17 | |
| α-helix | 61-101 | 41 | |
| α-helix | 107-138 | 32 | |
| α-helix | 201-226 | 26 | |
Chain E: 2 helices, 31 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 69-71 | 3 | 4 |
| β-strand | 77-81 | 5 | 4 |
| α-helix | 83-85 | 3 | |
| β-strand | 87-91 | 5 | 4 |
| β-strand | 102-105 | 4 | 4 |
| β-strand | 117-120 | 4 | 5 |
| β-strand | 127-131 | 5 | 5 |
| β-strand | 137-141 | 5 | 5 |
| β-strand | 154 | 1 | 5 |
| β-strand | 165-168 | 4 | 6 |
| β-strand | 174-178 | 5 | 6 |
| β-strand | 183-188 | 6 | 6 |
| α-helix | 190-192 | 3 | |
| β-strand | 194-200 | 7 | 6 |
| β-strand | 212-217 | 6 | 7 |
| β-strand | 219 | 1 | 8 |
| β-strand | 221 | 1 | 8 |
| β-strand | 223-228 | 6 | 7 |
| β-strand | 233-237 | 5 | 7 |
| β-strand | 238 | 1 | 9 |
| β-strand | 243 | 1 | 9 |
| β-strand | 247-249 | 3 | 7 |
| β-strand | 258-261 | 4 | 10 |
| β-strand | 267-271 | 5 | 10 |
| β-strand | 277-281 | 5 | 10 |
| β-strand | 287-290 | 4 | 10 |
| β-strand | 301-303 | 3 | 11 |
| β-strand | 309-313 | 5 | 11 |
| β-strand | 319-323 | 5 | 11 |
| β-strand | 329-332 | 4 | 11 |
| β-strand | 344-346 | 3 | 12 |
| β-strand | 353-356 | 4 | 12 |
| β-strand | 362-365 | 4 | 12 |
Chain M: 18 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 64-67 | 4 | |
| α-helix | 71-79 | 9 | |
| α-helix | 87-96 | 10 | |
| β-strand | 102-105 | 4 | 13 |
| α-helix | 113-123 | 11 | |
| β-strand | 133-136 | 4 | 13 |
| α-helix | 140-153 | 14 | |
| β-strand | 162-165 | 4 | 13 |
| α-helix | 171-179 | 9 | |
| β-strand | 187-190 | 4 | 13 |
| α-helix | 192-199 | 8 | |
| β-strand | 211-215 | 5 | 13 |
| α-helix | 217-222 | 6 | |
| α-helix | 224-236 | 13 | |
| β-strand | 242-247 | 6 | 13 |
| α-helix | 254-258 | 5 | |
| β-strand | 266-268 | 3 | 13 |
| β-strand | 283-287 | 5 | 14 |
| α-helix | 292-295 | 4 | |
| α-helix | 297-299 | 3 | |
| β-strand | 309-312 | 4 | 14 |
| α-helix | 316-326 | 11 | |
| β-strand | 333-335 | 3 | 14 |
| α-helix | 342-353 | 12 | |
| β-strand | 359-362 | 4 | 14 |
| α-helix | 364-366 | 3 | |
| β-strand | 377-380 | 4 | 14 |
| α-helix | 387-396 | 10 | |
| β-strand | 406-410 | 5 | 14 |
| α-helix | 414-426 | 13 | |
| β-strand | 431-432 | 2 | 14 |
| α-helix | 440-442 | 3 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| THO complex subunit HPR1 | A | protein | 603 | Saccharomyces cerevisiae | P17629 (AlphaFold model) |
| THO complex subunit THP2 | B | protein | 261 | Saccharomyces cerevisiae | O13539 (AlphaFold model) |
| THO complex subunit 2 | C | protein | 1262 | Saccharomyces cerevisiae | P53552 (AlphaFold model) |
| THO complex subunit MFT1 | D | protein | 256 | Saccharomyces cerevisiae | P33441 (AlphaFold model) |
| Tex1 | E | protein | 385 | Saccharomyces bayanus | A0ABN8WJ72 |
| ATP-dependent RNA helicase SUB2 | M | protein | 446 | Saccharomyces cerevisiae | Q07478 |
Sequence of entity 1 (A), FASTA
>7LUV_1 THO complex subunit HPR1 (chains A)
MSNTEELIQNSIGFLQKTFKALPVSFDSIRHEPLPSSMLHASVLNFEWEPLEKNISAIHD
RDSLIDIILKRFIIDSMTNAIEDEEENNLEKGLLNSCIGLDFVYNSRFNRSNPASWGNTF
FELFSTIIDLLNSPSTFLKFWPYAESRIEWFKMNTSVEPVSLGESNLISYKQPLYEKLRH
WNDILAKLENNDILNTVKHYNMKYKLENFLSELLPINEESNFNRSASISALQESDNEWNR
SARERESNRSSDVIFAADYNFVFYHLIICPIEFAFSDLEYKNDVDRSLSPLLDAILEIEE
NFYSKIKMNNRTRYSLEEALNTEYYANYDVMTPKLPVYMKHSNAMKMDRNEFWANLQNIK
ESDDYTLRPTIMDISLSNTTCLYKQLTQEDDDYYRKQFILQLCFTTNLIRNLISSDETRN
FYKSCYLRENPLSDIDFENLDEVNKKRGLNLCSYICDNRVLKFYKIKDPDFYRVIRKLMS
SDEKFTTAKIDGFKEFQNFRISKEKIPPPAFDETFKKFTFIKMGNKLINNVWKIPTGLDK
IEQEVKKPEGVYEAAQAKWESKISSETSGGEAKDEIIRQWQTLRFLRSRYLFDFDKVNEK
TGV
Sequence of entity 2 (B), FASTA
>7LUV_2 THO complex subunit THP2 (chains B)
MTKEEGRTYFESLCEEEQSLQESQTHLLNILDILSVLADPRSSDDLLTESLKKLPDLHRE
LINSSIRLRYDKYQTREAQLLEDTKTGRDVAAGVQNPKSISEYYSTFEHLNRDTLRYINL
LKRLSVDLAKQVEVSDPSVTVYEMDKWVPSEKLQGILEQYCAPDTDIRGVDAQIKNYLDQ
IKMARAKFGLENKYSLKERLSTLTKELNHWRKEWDDIEMLMFGDDAHSMKKMIQKIDSLK
SEINAPSESYPVDKEGDIVLE
Sequence of entity 3 (C), FASTA
>7LUV_3 THO complex subunit 2 (chains C)
GAMGSMAEQTLLSKLNALSQKVIPPASPSQASILTEEVIRNXXXXXXXXXXXXXXXESND
EKEDWLRTLFIELFDFINKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXLTTISLCKLIPSLHEELFKFSWISSKLLNKEQTTLLRHLLKKSKYELKKYNLL
VENSVGYGQLVALLILAYYDPDNFSKVSAYLKEIYHIMGKYSLDSIRTLDVILNVSSQFI
TEGYKFFIALLRKSDSWPSSHVANNSNYSSLNEGGNMIAANIISFNLSQYNEEVDKENYE
RYMDMCCILLKNGFVNFYSIWDNVKPEMEFLQEYIQNLETELEEESTKGVENPLAMAAAL
STENETDEDNALVVNDDVNMKDKISEETNADIESKGKQKTQQDILLFGKIKLLERLLIHG
CVIPVIHVLKQYPKVLYVSESLSRYLGRVFEYLLNPLYTSMTSSGESKDMATALMITRID
NGILAHKPRLIHKYKTHEPFESLELNSSYVFYYSEWNSNLTPFASVNDLFENSHIYLSII
GPYLGRIPTLLSKISRIGVADIQKNHGSESLHVTIDKWIDYVRKFIFPATSLLQNNPIAT
SEVYELMKFFPFEKRYFIYNEMMTKLSQDILPLKVSFNKAEREAKSILKALSIDTIAKES
RRFAKLISTNPLASLVPAVKQIENYDKVSELVVYTTKYFNDFAYDVLQFVLLLRLTYNRP
AVQFDGVNQAMWVQRLSIFIAGLAKNCPNMDISNIITYILKTLHNGNIIAVSILKELIIT
VGGIRDLNEVNMKQLLMLNSGSPLKQYARHLIYDFRDDNSVISSRLTSFFTDQSAISEII
LLLYTLNLKANTQNSHYKILSTRCDEMNTLLWSFIELIKHCLKGKAFEENVLPFVELNNR
FHLSTPWTFHIWRDYLDNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXL
KTLLFCCTSSEAGNLGLFFTDVLKKLEKMRLNGDFNDQASRKLYEWHSVITEQVIDLLSE
KNYMSIRNGIEFMKHVTSVFPVVKAHIQLVYTTLEENLINEEREDIKLPSSALIGHLKAR
LKDALELDEFCTLTEEEAEQKRIREMELEEIKNYETACQNEQKQVALRKQLELNKSQRLQ
ND
Sequence of entity 4 (D), FASTA
>7LUV_4 THO complex subunit MFT1 (chains D)
MPLSQKQIDQVRTKVHYSEVDTPFNKYLDILGKVTKLTGSIINGTLSNDDSKIEKLTEQN
ISQLKESAHLRFLDLQSSIDTKKVADENWETCQQETLAKLENLKDKLPDIKSIHSKLLLR
IGKLQGLYDSVQVINREVEGLSEGRTSLVVTRAEWEKELGTDLVKFLIEKNYLKLVDPGL
KKDSSEERYRIYDDFSKGPKELESINASMKSDIENVRQEVSSYKEKWLRDAEIFGKITSI
FKEELLKRDGLLNEAE
Sequence of entity 5 (E), FASTA
>7LUV_5 Tex1 (chains E)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXSLKFHASGSSMAYSRMDGSLTVWFIKDASFDKSVKVYIPDCCGSDKLA
TDLSWNPTSLNQMAVVSNSSEISLLLINEITLTASKLRTLSLGSKTKVNSCLYDPLGNWL
LAATKSEKIYLFNVKEDHRLVHSLNVNDISPNTNDVVYSIAWNNNGSHIFVGFKSGHLVI
LKLRDEMLEVSTKIKAHTSSITGIKIDPWGRYFVTGSSDGNCYIWSLKSLCCEKIIQDLD
SAVVALDVCHLGKILGVCTEDEMVYFYDLNEAKLLESKSLANHKSDLVLKFYPDKSWYIL
SGKNDTLSNHFVKNEKNLITYWKDM
Sequence of entity 6 (M), FASTA
>7LUV_6 ATP-dependent RNA helicase SUB2 (chains M)
MSHEGEEDLLEYSDNEQEIQIDASKAAEAGETGAATSATEGDNNNNTAAGDKKGSYVGIH
STGFKDFLLKPELSRAIIDCGFEHPSEVQQHTIPQSIHGTDVLCQAKSGLGKTAVFVLST
LQQLDPVPGEVAVVVICNARELAYQIRNEYLRFSKYMPDVKTAVFYGGTPISKDAELLKN
KDTAPHIVVATPGRLKALVREKYIDLSHVKNFVIDECDKVLEELDMRRDVQEIFRATPRD
KQVMMFSATLSQEIRPICRRFLQNPLEIFVDDEAKLTLHGLQQYYIKLEEREKNRKLAQL
LDDLEFNQVIIFVKSTTRANELTKLLNASNFPAITVHGHMKQEERIARYKAFKDFEKRIC
VSTDVFGRGIDIERINLAINYDLTNEADQYLHRVGRAGRFGTKGLAISFVSSKEDEEVLA
KIQERFDVKIAEFPEEGIDPSTYLNN
Primary citation
Cryo-EM structure of the yeast TREX complex and coordination with the SR-like protein Gbp2. Xie, Y., Clarke, B.P., Kim, Y.J. et al. Elife (2021) 10. DOI 10.7554/eLife.65699 · PubMed
Other PDB entries of the same protein (UniProt P17629 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7V2W 3.2 Å, protomer structure from the dimer of yeast THO complex
- 7APX 3.4 Å, yeast THO-Sub2 complex
- 7V2Y 3.4 Å, cryo-EM structure of yeast THO complex with Sub2
- 7AQO 4.5 Å, yeast THO-Sub2 complex dimer
Browse structure collections
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