7V2Y: Yeast THO complex with Sub2
cryo-EM structure of yeast THO complex with Sub2. Determined by electron microscopy at 3.4 Å resolution. Released 27 Jul 2022.
- Method
- Electron microscopy
- Resolution
- 3.4 Å
- Organism
- Saccharomyces cerevisiae S288c
- Chains
- 6
- Atoms
- 23,097
- Mol. weight
- 445.1 kDa
- Released
- 27 Jul 2022
Explore 7V2Y in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7V2Y contains 144 α-helices and 53 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 34 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-19 | 16 | |
| α-helix | 26-31 | 6 | |
| α-helix | 33-35 | 3 | |
| α-helix | 43-45 | 3 | |
| α-helix | 50-53 | 4 | |
| α-helix | 61-78 | 18 | |
| α-helix | 85-105 | 21 | |
| α-helix | 116-130 | 15 | |
| α-helix | 135-138 | 4 | |
| α-helix | 142-146 | 5 | |
| α-helix | 149-152 | 4 | |
| α-helix | 159-160 | 2 | |
| α-helix | 178-190 | 13 | |
| α-helix | 197-211 | 15 | |
| α-helix | 227-229 | 3 | |
| α-helix | 251-262 | 12 | |
| α-helix | 263-267 | 5 | |
| α-helix | 271-274 | 4 | |
| α-helix | 282-295 | 14 | |
| α-helix | 299-320 | 22 | |
| α-helix | 343-350 | 8 | |
| α-helix | 352-359 | 8 | |
| α-helix | 379-387 | 9 | |
| α-helix | 396-414 | 19 | |
| α-helix | 417-421 | 5 | |
| α-helix | 423-426 | 4 | |
| α-helix | 445-455 | 11 | |
| α-helix | 464-467 | 4 | |
| α-helix | 472-491 | 20 | |
| α-helix | 506-509 | 4 | |
| α-helix | 514-517 | 4 | |
| α-helix | 526-531 | 6 | |
| α-helix | 548-564 | 17 | |
| α-helix | 575-582 | 8 | |
Chain B: 72 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-14 | 13 | |
| α-helix | 37-49 | 13 | |
| α-helix | 54-71 | 18 | |
| α-helix | 87-100 | 14 | |
| α-helix | 105-114 | 10 | |
| α-helix | 122-129 | 8 | |
| α-helix | 136-141 | 6 | |
| α-helix | 144-146 | 3 | |
| α-helix | 155-161 | 7 | |
| β-strand | 172 | 1 | 1 |
| α-helix | 181-191 | 11 | |
| α-helix | 203-215 | 13 | |
| β-strand | 218 | 1 | 1 |
| α-helix | 220-230 | 11 | |
| α-helix | 231-233 | 3 | |
| α-helix | 241-247 | 7 | |
| α-helix | 274-281 | 8 | |
| α-helix | 294-307 | 14 | |
| α-helix | 313-316 | 4 | |
| α-helix | 323-342 | 20 | |
| α-helix | 404-414 | 11 | |
| α-helix | 418-426 | 9 | |
| α-helix | 428-430 | 3 | |
| α-helix | 435-437 | 3 | |
| α-helix | 440-447 | 8 | |
| α-helix | 450-456 | 7 | |
| α-helix | 458-462 | 5 | |
| α-helix | 464-467 | 4 | |
| β-strand | 473-474 | 2 | 2 |
| β-strand | 481-482 | 2 | 2 |
| β-strand | 484-486 | 3 | 3 |
| β-strand | 488-491 | 4 | 4 |
| β-strand | 497-498 | 2 | 4 |
| β-strand | 503-506 | 4 | 4 |
| α-helix | 528-531 | 4 | |
| α-helix | 539-541 | 3 | |
| α-helix | 543-559 | 17 | |
| α-helix | 568-572 | 5 | |
| α-helix | 574-580 | 7 | |
| α-helix | 593-602 | 10 | |
| α-helix | 611-616 | 6 | |
| α-helix | 626-639 | 14 | |
| α-helix | 651-664 | 14 | |
| α-helix | 671-675 | 5 | |
| α-helix | 684-690 | 7 | |
| α-helix | 691-693 | 3 | |
| α-helix | 698-710 | 13 | |
| α-helix | 732-741 | 10 | |
| α-helix | 748-757 | 10 | |
| α-helix | 763-775 | 13 | |
| α-helix | 787-792 | 6 | |
| α-helix | 798-806 | 9 | |
| α-helix | 815-827 | 13 | |
| α-helix | 831-844 | 14 | |
| α-helix | 858-876 | 19 | |
| α-helix | 882-885 | 4 | |
| α-helix | 887-888 | 2 | |
| α-helix | 889-892 | 4 | |
| α-helix | 900-915 | 16 | |
| α-helix | 919-926 | 8 | |
| α-helix | 942-950 | 9 | |
| α-helix | 961-973 | 13 | |
| α-helix | 980-1018 | 39 | |
| α-helix | 1028-1030 | 3 | |
| α-helix | 1031-1035 | 5 | |
| α-helix | 1036-1038 | 3 | |
| α-helix | 1043-1046 | 4 | |
| α-helix | 1048-1051 | 4 | |
| α-helix | 1053-1056 | 4 | |
| α-helix | 1059-1070 | 12 | |
| α-helix | 1075-1078 | 4 | |
| α-helix | 1086-1106 | 21 | |
| α-helix | 1112-1133 | 22 | |
| α-helix | 1139-1150 | 12 | |
| α-helix | 1159-1174 | 16 | |
| α-helix | 1182-1192 | 11 | |
| α-helix | 1193-1195 | 3 | |
| α-helix | 1202-1205 | 4 | |
| α-helix | 1212-1231 | 20 | |
| α-helix | 1242-1254 | 13 | |
Chain C: 3 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 43-44 | 2 | 5 |
| α-helix | 50-55 | 6 | |
| β-strand | 69-70 | 2 | 6 |
| β-strand | 79-80 | 2 | 6 |
| β-strand | 88-89 | 2 | 6 |
| β-strand | 115-120 | 6 | 7 |
| α-helix | 121 | 1 | |
| β-strand | 128-132 | 5 | 7 |
| β-strand | 137-142 | 6 | 7 |
| β-strand | 149-155 | 7 | 7 |
| β-strand | 163-165 | 3 | 8 |
| β-strand | 168 | 1 | 9 |
| β-strand | 174-175 | 2 | 9 |
| β-strand | 178-179 | 2 | 8 |
| β-strand | 183-186 | 4 | 8 |
| β-strand | 187-188 | 2 | 9 |
| β-strand | 197-200 | 4 | 8 |
| α-helix | 201-203 | 3 | |
| β-strand | 214-217 | 4 | 10 |
| β-strand | 223-227 | 5 | 10 |
| β-strand | 232-238 | 7 | 10 |
| β-strand | 243-250 | 8 | 10 |
| β-strand | 259-261 | 3 | 11 |
| β-strand | 267-270 | 4 | 11 |
| β-strand | 278-281 | 4 | 11 |
| β-strand | 287-290 | 4 | 11 |
| β-strand | 298-302 | 5 | 12 |
| β-strand | 311-314 | 4 | 12 |
| β-strand | 318-320 | 3 | 12 |
| β-strand | 334-336 | 3 | 12 |
| β-strand | 344-346 | 3 | 5 |
| β-strand | 353-356 | 4 | 5 |
| β-strand | 363-366 | 4 | 5 |
Chain D: 5 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-8 | 4 | |
| α-helix | 11-14 | 4 | |
| α-helix | 22-43 | 22 | |
| α-helix | 59-99 | 41 | |
| α-helix | 107-137 | 31 | |
Chain E: 9 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-8 | 3 | |
| α-helix | 9-36 | 28 | |
| α-helix | 46-52 | 7 | |
| α-helix | 54-83 | 30 | |
| α-helix | 97-103 | 7 | |
| α-helix | 105-108 | 4 | |
| α-helix | 110-118 | 9 | |
| β-strand | 141-143 | 3 | 3 |
| α-helix | 151-161 | 11 | |
| α-helix | 169-183 | 15 | |
Chain F: 21 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 65-67 | 3 | |
| α-helix | 75-80 | 6 | |
| α-helix | 89-97 | 9 | |
| β-strand | 102-105 | 4 | 13 |
| α-helix | 113-123 | 11 | |
| α-helix | 124-126 | 3 | |
| β-strand | 133-136 | 4 | 13 |
| α-helix | 140-150 | 11 | |
| β-strand | 162-165 | 4 | 13 |
| α-helix | 171-179 | 9 | |
| α-helix | 181-183 | 3 | |
| β-strand | 187-190 | 4 | 13 |
| α-helix | 192-199 | 8 | |
| β-strand | 211-215 | 5 | 13 |
| α-helix | 217-221 | 5 | |
| α-helix | 225-235 | 11 | |
| β-strand | 242-247 | 6 | 13 |
| α-helix | 255-258 | 4 | |
| α-helix | 265 | 1 | |
| β-strand | 266-267 | 2 | 13 |
| β-strand | 282-287 | 6 | 14 |
| α-helix | 297-301 | 5 | |
| β-strand | 309-312 | 4 | 14 |
| α-helix | 318-327 | 10 | |
| β-strand | 333-335 | 3 | 14 |
| α-helix | 342-353 | 12 | |
| β-strand | 359-362 | 4 | 14 |
| α-helix | 364-367 | 4 | |
| β-strand | 377-380 | 4 | 14 |
| α-helix | 390-393 | 4 | |
| α-helix | 399-401 | 3 | |
| β-strand | 405-410 | 6 | 14 |
| α-helix | 415-425 | 11 | |
| β-strand | 431-432 | 2 | 14 |
| α-helix | 433-434 | 2 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| THO complex subunit HPR1 | A | protein | 752 | Saccharomyces cerevisiae S288c | P17629 (AlphaFold model) |
| THO complex subunit 2 | B | protein | 1597 | Saccharomyces cerevisiae S288c | P53552 (AlphaFold model) |
| Protein TEX1 | C | protein | 422 | Saccharomyces cerevisiae S288c | P53851 (AlphaFold model) |
| THO complex subunit MFT1 | D | protein | 392 | Saccharomyces cerevisiae S288c | P33441 (AlphaFold model) |
| THO complex subunit THP2 | E | protein | 261 | Saccharomyces cerevisiae S288c | O13539 |
| ATP-dependent RNA helicase SUB2 | F | protein | 446 | Saccharomyces cerevisiae S288c | Q07478 |
Sequence of entity 1 (A), FASTA
>7V2Y_1 THO complex subunit HPR1 (chains A)
MSNTEELIQNSIGFLQKTFKALPVSFDSIRHEPLPSSMLHASVLNFEWEPLEKNISAIHD
RDSLIDIILKRFIIDSMTNAIEDEEENNLEKGLLNSCIGLDFVYNSRFNRSNPASWGNTF
FELFSTIIDLLNSPSTFLKFWPYAESRIEWFKMNTSVEPVSLGESNLISYKQPLYEKLRH
WNDILAKLENNDILNTVKHYNMKYKLENFLSELLPINEESNFNRSASISALQESDNEWNR
SARERESNRSSDVIFAADYNFVFYHLIICPIEFAFSDLEYKNDVDRSLSPLLDAILEIEE
NFYSKIKMNNRTRYSLEEALNTEYYANYDVMTPKLPVYMKHSNAMKMDRNEFWANLQNIK
ESDDYTLRPTIMDISLSNTTCLYKQLTQEDDDYYRKQFILQLCFTTNLIRNLISSDETRN
FYKSCYLRENPLSDIDFENLDEVNKKRGLNLCSYICDNRVLKFYKIKDPDFYRVIRKLMS
SDEKFTTAKIDGFKEFQNFRISKEKIPPPAFDETFKKFTFIKMGNKLINNVWKIPTGLDK
IEQEVKKPEGVYEAAQAKWESKISSETSGGEAKDEIIRQWQTLRFLRSRYLFDFDKVNEK
TGVDGLFEEPRKVEALDDSFKEKLLYKINQEHRKKLQDAREYKIGKERKKRALEEEASFP
EREQKIKSQRINSASQTEGDELKSEQTQPKGEISEENTKIKSSEVSSQDPDSGVAGEFAP
QNTTAQLENPKTEDNNAATSNISNGSSTQDMK
Sequence of entity 2 (B), FASTA
>7V2Y_2 THO complex subunit 2 (chains B)
MAEQTLLSKLNALSQKVIPPASPSQASILTEEVIRNWPERSKTLCSDFTALESNDEKEDW
LRTLFIELFDFINKNDENSPLKLSDVASFTNELVNHERQVSQASIVGKMFIAVSSTVPNI
NDLTTISLCKLIPSLHEELFKFSWISSKLLNKEQTTLLRHLLKKSKYELKKYNLLVENSV
GYGQLVALLILAYYDPDNFSKVSAYLKEIYHIMGKYSLDSIRTLDVILNVSSQFITEGYK
FFIALLRKSDSWPSSHVANNSNYSSLNEGGNMIAANIISFNLSQYNEEVDKENYERYMDM
CCILLKNGFVNFYSIWDNVKPEMEFLQEYIQNLETELEEESTKGVENPLAMAAALSTENE
TDEDNALVVNDDVNMKDKISEETNADIESKGKQKTQQDILLFGKIKLLERLLIHGCVIPV
IHVLKQYPKVLYVSESLSRYLGRVFEYLLNPLYTSMTSSGESKDMATALMITRIDNGILA
HKPRLIHKYKTHEPFESLELNSSYVFYYSEWNSNLTPFASVNDLFENSHIYLSIIGPYLG
RIPTLLSKISRIGVADIQKNHGSESLHVTIDKWIDYVRKFIFPATSLLQNNPIATSEVYE
LMKFFPFEKRYFIYNEMMTKLSQDILPLKVSFNKAEREAKSILKALSIDTIAKESRRFAK
LISTNPLASLVPAVKQIENYDKVSELVVYTTKYFNDFAYDVLQFVLLLRLTYNRPAVQFD
GVNQAMWVQRLSIFIAGLAKNCPNMDISNIITYILKTLHNGNIIAVSILKELIITVGGIR
DLNEVNMKQLLMLNSGSPLKQYARHLIYDFRDDNSVISSRLTSFFTDQSAISEIILLLYT
LNLKANTQNSHYKILSTRCDEMNTLLWSFIELIKHCLKGKAFEENVLPFVELNNRFHLST
PWTFHIWRDYLDNQLNSNENFSIDELIEGAEFSDVDLTKISKDLFTTFWRLSLYDIHFDK
SLYDERKNALSGENTGHMSNRKKHLIQNQIKDILVTGISHQRAFKKTSEFISEKSNVWNK
DCGEDQIKIFLQNCVVPRVLFSPSDALFSSFFIFMAFRTENLMSILNTCITSNILKTLLF
CCTSSEAGNLGLFFTDVLKKLEKMRLNGDFNDQASRKLYEWHSVITEQVIDLLSEKNYMS
IRNGIEFMKHVTSVFPVVKAHIQLVYTTLEENLINEEREDIKLPSSALIGHLKARLKDAL
ELDEFCTLTEEEAEQKRIREMELEEIKNYETACQNEQKQVALRKQLELNKSQRLQNDPPK
SVASGSAGLNSKDRYTYSRNEPVIPTKPSSSQWSYSKVTRHVDDINHYLATNHLQKAISL
VENDDETRNLRKLSKQNMPIFDFRNSTLEIFERYFRTLIQNPQNPDFAEKIDSLKRYIKN
ISREPYPDTTSSYSEAAAPEYTKRSSRYSGNAGGKDGYGSSNYRGPSNDRSAPKNIKPIS
SYAHKRSELPTRPSKSKTYNDRSRALRPTGPDRGDGFDQRDNRLREEYKKNSSQRSQLRF
PEKPFQEGKDSSKANPYQASSYKRDSPSENEEKPNKRFKKDETIRNKFQTQDYRNTRDSG
AAHRANENQRYNGNRKSNTQALPQGPKGGNYVSRYQR
Sequence of entity 3 (C), FASTA
>7V2Y_3 Protein TEX1 (chains C)
MSTIGAVDILNQKTITSEVAASVTSKYLQSTFSKGNTSHIEDKRFIHVSSRSHSRFTSTP
ITPNEILSLKFHVSGSSMAYSRMDGSLTVWFIKDASFDKSVEVYIPDCCGSDKLATDLSW
NPTSLNQIAVVSNSSEISLLLINEKSLTASKLRTLSLGSKTKVNTCLYDPLGNWLLAATK
SEKIYLFDVKKDHSSVCSLNISDISQEDNDVVYSLAWSNGGSHIFIGFKSGYLAILKAKH
GILEVCTKIKAHTGPITEIKMDPWGRNFITGSIDGNCYVWNMKSLCCELIINDLNSAVTT
LDVCHLGKILGICTEDEMVYFYDLNSGNLLHSKSLANYKTDPVLKFYPDKSWYIMSGKND
TLSNHFVKNEKNLITYWKDMFDNTMIEKRRKNNGGGNNHNKRTSKNTDRIGKDRPSRFNS
KK
Sequence of entity 4 (D), FASTA
>7V2Y_4 THO complex subunit MFT1 (chains D)
MPLSQKQIDQVRTKVHYSEVDTPFNKYLDILGKVTKLTGSIINGTLSNDDSKIEKLTEQN
ISQLKESAHLRFLDLQSSIDTKKVADENWETCQQETLAKLENLKDKLPDIKSIHSKLLLR
IGKLQGLYDSVQVINREVEGLSEGRTSLVVTRAEWEKELGTDLVKFLIEKNYLKLVDPGL
KKDSSEERYRIYDDFSKGPKELESINASMKSDIENVRQEVSSYKEKWLRDAEIFGKITSI
FKEELLKRDGLLNEAEGDNIDEDYESDEDEERKERFKRQRSMVEVNTIENVDEKEESDHE
YDDQEDEENEEEDDMEVDVEDIKEDNEVDGESSQQEDNSRQGNNEETDKETGVIEEPDAV
NDAEEADSDHSSRKLGGTTSDFSASSSVEEVK
Sequence of entity 5 (E), FASTA
>7V2Y_5 THO complex subunit THP2 (chains E)
MTKEEGRTYFESLCEEEQSLQESQTHLLNILDILSVLADPRSSDDLLTESLKKLPDLHRE
LINSSIRLRYDKYQTREAQLLEDTKTGRDVAAGVQNPKSISEYYSTFEHLNRDTLRYINL
LKRLSVDLAKQVEVSDPSVTVYEMDKWVPSEKLQGILEQYCAPDTDIRGVDAQIKNYLDQ
IKMARAKFGLENKYSLKERLSTLTKELNHWRKEWDDIEMLMFGDDAHSMKKMIQKIDSLK
SEINAPSESYPVDKEGDIVLE
Sequence of entity 6 (F), FASTA
>7V2Y_6 ATP-dependent RNA helicase SUB2 (chains F)
MSHEGEEDLLEYSDNEQEIQIDASKAAEAGETGAATSATEGDNNNNTAAGDKKGSYVGIH
STGFKDFLLKPELSRAIIDCGFEHPSEVQQHTIPQSIHGTDVLCQAKSGLGKTAVFVLST
LQQLDPVPGEVAVVVICNARELAYQIRNEYLRFSKYMPDVKTAVFYGGTPISKDAELLKN
KDTAPHIVVATPGRLKALVREKYIDLSHVKNFVIDECDKVLEELDMRRDVQEIFRATPRD
KQVMMFSATLSQEIRPICRRFLQNPLEIFVDDEAKLTLHGLQQYYIKLEEREKNRKLAQL
LDDLEFNQVIIFVKSTTRANELTKLLNASNFPAITVHGHMKQEERIARYKAFKDFEKRIC
VSTDVFGRGIDIERINLAINYDLTNEADQYLHRVGRAGRFGTKGLAISFVSSKEDEEVLA
KIQERFDVKIAEFPEEGIDPSTYLNN
Primary citation
Structural and functional insights into R-loop prevention and mRNA export by budding yeast THO-Sub2 complex. Chen, C., Tan, M., Wu, Z. et al. Sci Bull (Beijing) (2021) 66:2347-2352. DOI 10.1016/j.scib.2021.08.004 · PubMed
Other PDB entries of the same protein (UniProt P17629 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7V2W 3.2 Å, protomer structure from the dimer of yeast THO complex
- 7APX 3.4 Å, yeast THO-Sub2 complex
- 7LUV 3.7 Å, Cryo-EM structure of the yeast THO-Sub2 complex
- 7AQO 4.5 Å, yeast THO-Sub2 complex dimer
Browse structure collections
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