7AQO: Yeast THO-Sub2 complex dimer
yeast THO-Sub2 complex dimer. Determined by electron microscopy at 4.5 Å resolution. Released 2 Dec 2020.
- Method
- Electron microscopy
- Resolution
- 4.5 Å
- Organism
- Saccharomyces cerevisiae S288C
- Chains
- 12
- Atoms
- 40,261
- Mol. weight
- 864.45 kDa
- Released
- 2 Dec 2020
Explore 7AQO in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7AQO contains 281 α-helices and 114 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains A and G: 70 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 37-50 | 14 | |
| α-helix | 54-71 | 18 | |
| α-helix | 86-100 | 15 | |
| α-helix | 106-114 | 9 | |
| α-helix | 124-131 | 8 | |
| α-helix | 136-142 | 7 | |
| α-helix | 152-165 | 14 | |
| β-strand | 172 | 1 | 18 |
| α-helix | 179-193 | 15 | |
| α-helix | 198-201 | 4 | |
| α-helix | 202-215 | 14 | |
| β-strand | 218 | 1 | 18 |
| α-helix | 220-232 | 13 | |
| α-helix | 235-248 | 14 | |
| α-helix | 263-265 | 3 | |
| β-strand | 270 | 1 | 19 |
| α-helix | 272-281 | 10 | |
| α-helix | 290-292 | 3 | |
| α-helix | 294-304 | 11 | |
| β-strand | 310 | 1 | 19 |
| α-helix | 313-316 | 4 | |
| α-helix | 323-342 | 20 | |
| α-helix | 395-401 | 7 | |
| α-helix | 404-413 | 10 | |
| α-helix | 417-424 | 8 | |
| α-helix | 428-432 | 5 | |
| α-helix | 436-455 | 20 | |
| α-helix | 468-470 | 3 | |
| β-strand | 471-475 | 5 | 20 |
| β-strand | 478-482 | 5 | 20 |
| β-strand | 484-485 | 2 | 21 |
| β-strand | 487-491 | 5 | 22 |
| β-strand | 497-498 | 2 | 22 |
| β-strand | 502-506 | 5 | 22 |
| α-helix | 511-513 | 3 | |
| α-helix | 521-535 | 15 | |
| α-helix | 536-541 | 6 | |
| α-helix | 543-559 | 17 | |
| α-helix | 566-576 | 11 | |
| α-helix | 577-581 | 5 | |
| α-helix | 582-585 | 4 | |
| α-helix | 592-602 | 11 | |
| α-helix | 607-619 | 13 | |
| α-helix | 626-643 | 18 | |
| α-helix | 651-664 | 14 | |
| α-helix | 666-669 | 4 | |
| α-helix | 671-679 | 9 | |
| α-helix | 683-690 | 8 | |
| α-helix | 691-693 | 3 | |
| α-helix | 696-711 | 16 | |
| β-strand | 717 | 1 | 23 |
| α-helix | 723 | 1 | |
| β-strand | 724 | 1 | 23 |
| α-helix | 725 | 1 | |
| α-helix | 728-741 | 14 | |
| α-helix | 748-757 | 10 | |
| α-helix | 758-760 | 3 | |
| α-helix | 763-772 | 10 | |
| α-helix | 773-777 | 5 | |
| α-helix | 787-792 | 6 | |
| α-helix | 797-806 | 10 | |
| α-helix | 815-826 | 12 | |
| α-helix | 827-829 | 3 | |
| α-helix | 831-846 | 16 | |
| α-helix | 852-876 | 25 | |
| α-helix | 879-884 | 6 | |
| α-helix | 889-894 | 6 | |
| α-helix | 900-916 | 17 | |
| α-helix | 942-950 | 9 | |
| α-helix | 961-969 | 9 | |
| α-helix | 984-992 | 9 | |
| α-helix | 994-1017 | 24 | |
| α-helix | 1025-1030 | 6 | |
| α-helix | 1031-1035 | 5 | |
| α-helix | 1036-1039 | 4 | |
| α-helix | 1043-1053 | 11 | |
| α-helix | 1059-1070 | 12 | |
| α-helix | 1075-1081 | 7 | |
| α-helix | 1084-1106 | 23 | |
| α-helix | 1114-1132 | 19 | |
| α-helix | 1138-1151 | 14 | |
| α-helix | 1152-1154 | 3 | |
Chains B and H: 33 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-21 | 19 | |
| α-helix | 26-31 | 6 | |
| α-helix | 33-35 | 3 | |
| α-helix | 36-38 | 3 | |
| α-helix | 44-46 | 3 | |
| α-helix | 56-58 | 3 | |
| α-helix | 62-77 | 16 | |
| α-helix | 92-105 | 14 | |
| α-helix | 113-115 | 3 | |
| α-helix | 116-130 | 15 | |
| α-helix | 134-146 | 13 | |
| α-helix | 148-151 | 4 | |
| α-helix | 159-161 | 3 | |
| α-helix | 174-191 | 18 | |
| α-helix | 197-213 | 17 | |
| α-helix | 251-262 | 12 | |
| α-helix | 263-268 | 6 | |
| α-helix | 272-274 | 3 | |
| α-helix | 280-319 | 40 | |
| α-helix | 337-340 | 4 | |
| α-helix | 344-360 | 17 | |
| α-helix | 368-370 | 3 | |
| α-helix | 379-386 | 8 | |
| α-helix | 393-415 | 23 | |
| α-helix | 418-429 | 12 | |
| α-helix | 443-452 | 10 | |
| α-helix | 453-461 | 9 | |
| α-helix | 462-463 | 2 | |
| α-helix | 471-490 | 20 | |
| α-helix | 494-496 | 3 | |
| α-helix | 506-509 | 4 | |
| α-helix | 526-531 | 6 | |
| α-helix | 577-586 | 10 | |
Chain C: 9 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-38 | 30 | |
| α-helix | 46-83 | 38 | |
| α-helix | 87-90 | 4 | |
| α-helix | 91-93 | 3 | |
| α-helix | 97-119 | 23 | |
| β-strand | 141-142 | 2 | 21 |
| α-helix | 151-158 | 8 | |
| α-helix | 159-161 | 3 | |
| α-helix | 167-193 | 27 | |
| α-helix | 195-234 | 40 | |
Chain D: 8 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-15 | 11 | |
| α-helix | 22-43 | 22 | |
| α-helix | 61-101 | 41 | |
| α-helix | 104-106 | 3 | |
| α-helix | 107-142 | 36 | |
| α-helix | 154-159 | 6 | |
| α-helix | 164-168 | 5 | |
| α-helix | 199-246 | 48 | |
Chains E and K: 3 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 23-31 | 9 | |
| β-strand | 39 | 1 | 24 |
| α-helix | 43-52 | 10 | |
| β-strand | 66-71 | 6 | 25 |
| β-strand | 78-82 | 5 | 25 |
| β-strand | 87-91 | 5 | 25 |
| β-strand | 101-102 | 2 | 25 |
| β-strand | 117-119 | 3 | 26 |
| β-strand | 127-131 | 5 | 26 |
| β-strand | 136-142 | 7 | 26 |
| β-strand | 149-156 | 8 | 26 |
| β-strand | 163-168 | 6 | 27 |
| β-strand | 174-179 | 6 | 27 |
| β-strand | 184-188 | 5 | 27 |
| β-strand | 194-199 | 6 | 27 |
| α-helix | 201-205 | 5 | |
| β-strand | 214-217 | 4 | 28 |
| β-strand | 223-227 | 5 | 28 |
| β-strand | 233-237 | 5 | 28 |
| β-strand | 244-249 | 6 | 28 |
| β-strand | 256-261 | 6 | 29 |
| β-strand | 267-272 | 6 | 29 |
| β-strand | 277-281 | 5 | 29 |
| β-strand | 287-291 | 5 | 29 |
| β-strand | 298-303 | 6 | 30 |
| β-strand | 309-314 | 6 | 30 |
| β-strand | 319-323 | 5 | 30 |
| β-strand | 329-332 | 4 | 30 |
| β-strand | 344-346 | 3 | 24 |
| β-strand | 353-356 | 4 | 24 |
| β-strand | 362-363 | 2 | 24 |
Chains F and L: 18 helices, 16 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 63-67 | 5 | |
| α-helix | 71-78 | 8 | |
| α-helix | 87-96 | 10 | |
| β-strand | 102-104 | 3 | 31 |
| α-helix | 112-123 | 12 | |
| β-strand | 133-136 | 4 | 31 |
| α-helix | 140-153 | 14 | |
| β-strand | 162-165 | 4 | 31 |
| α-helix | 171-179 | 9 | |
| β-strand | 187-190 | 4 | 31 |
| α-helix | 192-200 | 9 | |
| β-strand | 211-215 | 5 | 31 |
| α-helix | 217-222 | 6 | |
| α-helix | 224-235 | 12 | |
| β-strand | 242-246 | 5 | 31 |
| β-strand | 281-283 | 3 | 32 |
| β-strand | 284-285 | 2 | 33 |
| β-strand | 286-287 | 2 | 34 |
| α-helix | 290-292 | 3 | |
| α-helix | 293-303 | 11 | |
| β-strand | 308-312 | 5 | 32 |
| α-helix | 316-328 | 13 | |
| β-strand | 333-336 | 4 | 32 |
| α-helix | 342-353 | 12 | |
| β-strand | 359-362 | 4 | 32 |
| α-helix | 364-366 | 3 | |
| β-strand | 375-380 | 6 | 32 |
| α-helix | 387-394 | 8 | |
| β-strand | 404-408 | 5 | 32 |
| β-strand | 409-410 | 2 | 34 |
| α-helix | 413-426 | 14 | |
| β-strand | 431-432 | 2 | 33 |
| α-helix | 433-434 | 2 | |
| α-helix | 440-442 | 3 | |
Chain I: 8 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-15 | 11 | |
| α-helix | 22-43 | 22 | |
| α-helix | 61-101 | 41 | |
| α-helix | 104-106 | 3 | |
| α-helix | 107-142 | 36 | |
| α-helix | 154-159 | 6 | |
| α-helix | 164-168 | 5 | |
| α-helix | 199-248 | 50 | |
Chain J: 8 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-38 | 30 | |
| α-helix | 46-83 | 38 | |
| α-helix | 87-90 | 4 | |
| α-helix | 91-93 | 3 | |
| α-helix | 97-119 | 23 | |
| β-strand | 141-142 | 2 | 4 |
| α-helix | 151-158 | 8 | |
| α-helix | 159-161 | 3 | |
| α-helix | 167-234 | 68 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| THO complex subunit 2 | A, G | protein | 1601 | Saccharomyces cerevisiae S288C | P53552 (AlphaFold model) |
| THO complex subunit HPR1 | B, H | protein | 720 | Saccharomyces cerevisiae S288C | P17629 (AlphaFold model) |
| TEX1 isoform 1 | E, K | protein | 380 | Saccharomyces cerevisiae S288C | P53851 (AlphaFold model) |
| EM14S01-3B_G0007820.mRNA.1.CDS.1 | F, L | protein | 400 | Saccharomyces cerevisiae S288C | Q07478 (AlphaFold model) |
| BJ4_G0025130.mRNA.1.CDS.1 | C, J | protein | 261 | Saccharomyces cerevisiae S288C | O13539 |
| THO complex subunit MFT1 | D, I | protein | 392 | Saccharomyces cerevisiae S288C | P33441 |
Sequence of entity 1 (A, G), FASTA
>7AQO_1 THO complex subunit 2 (chains A, G)
GAASMAEQTLLSKLNALSQKVIPPASPSQASILTEEVIRNWPERSKTLCSDFTALESNDE
KEDWLRTLFIELFDFINKNDENSPLKLSDVASFTNELVNHERQVSQASIVGKMFIAVSST
VPNINDLTTISLCKLIPSLHEELFKFSWISSKLLNKEQTTLLRHLLKKSKYELKKYNLLV
ENSVGYGQLVALLILAYYDPDNFSKVSAYLKEIYHIMGKYSLDSIRTLDVILNVSSQFIT
EGYKFFIALLRKSDSWPSSHVANNSNYSSLNEGGNMIAANIISFNLSQYNEEVDKENYER
YMDMCCILLKNGFVNFYSIWDNVKPEMEFLQEYIQNLETELEEESTKGVENPLAMAAALS
TENETDEDNALVVNDDVNMKDKISEETNADIESKGKQKTQQDILLFGKIKLLERLLIHGC
VIPVIHVLKQYPKVLYVSESLSRYLGRVFEYLLNPLYTSMTSSGESKDMATALMITRIDN
GILAHKPRLIHKYKTHEPFESLELNSSYVFYYSEWNSNLTPFASVNDLFENSHIYLSIIG
PYLGRIPTLLSKISRIGVADIQKNHGSESLHVTIDKWIDYVRKFIFPATSLLQNNPIATS
EVYELMKFFPFEKRYFIYNEMMTKLSQDILPLKVSFNKAEREAKSILKALSIDTIAKESR
RFAKLISTNPLASLVPAVKQIENYDKVSELVVYTTKYFNDFAYDVLQFVLLLRLTYNRPA
VQFDGVNQAMWVQRLSIFIAGLAKNCPNMDISNIITYILKTLHNGNIIAVSILKELIITV
GGIRDLNEVNMKQLLMLNSGSPLKQYARHLIYDFRDDNSVISSRLTSFFTDQSAISEIIL
LLYTLNLKANTQNSHYKILSTRCDEMNTLLWSFIELIKHCLKGKAFEENVLPFVELNNRF
HLSTPWTFHIWRDYLDNQLNSNENFSIDELIEGAEFSDVDLTKISKDLFTTFWRLSLYDI
HFDKSLYDERKNALSGENTGHMSNRKKHLIQNQIKDILVTGISHQRAFKKTSEFISEKSN
VWNKDCGEDQIKIFLQNCVVPRVLFSPSDALFSSFFIFMAFRTENLMSILNTCITSNILK
TLLFCCTSSEAGNLGLFFTDVLKKLEKMRLNGDFNDQASRKLYEWHSVITEQVIDLLSEK
NYMSIRNGIEFMKHVTSVFPVVKAHIQLVYTTLEENLINEEREDIKLPSSALIGHLKARL
KDALELDEFCTLTEEEAEQKRIREMELEEIKNYETACQNEQKQVALRKQLELNKSQRLQN
DPPKSVASGSAGLNSKDRYTYSRNEPVIPTKPSSSQWSYSKVTRHVDDINHYLATNHLQK
AISLVENDDETRNLRKLSKQNMPIFDFRNSTLEIFERYFRTLIQNPQNPDFAEKIDSLKR
YIKNISREPYPDTTSSYSEAAAPEYTKRSSRYSGNAGGKDGYGSSNYRGPSNDRSAPKNI
KPISSYAHKRSELPTRPSKSKTYNDRSRALRPTGPDRGDGFDQRDNRLREEYKKNSSQRS
QLRFPEKPFQEGKDSSKANPYQASSYKRDSPSENEEKPNKRFKKDETIRNKFQTQDYRNT
RDSGAAHRANENQRYNGNRKSNTQALPQGPKGGNYVSRYQR
Sequence of entity 2 (B, H), FASTA
>7AQO_2 THO complex subunit HPR1 (chains B, H)
MSNTEELIQNSIGFLQKTFKALPVSFDSIRHEPLPSSMLHASVLNFEWEPLEKNISAIHD
RDSLIDIILKRFIIDSMTNAIEDEEENNLEKGLLNSCIGLDFVYNSRFNRSNPASWGNTF
FELFSTIIDLLNSPSTFLKFWPYAESRIEWFKMNTSVEPVSLGESNLISYKQPLYEKLRH
WNDILAKLENNDILNTVKHYNMKYKLENFLSELLPINEESNFNRSASISALQESDNEWNR
SARERESNRSSDVIFAADYNFVFYHLIICPIEFAFSDLEYKNDVDRSLSPLLDAILEIEE
NFYSKIKMNNRTRYSLEEALNTEYYANYDVMTPKLPVYMKHSNAMKMDRNEFWANLQNIK
ESDDYTLRPTIMDISLSNTTCLYKQLTQEDDDYYRKQFILQLCFTTNLIRNLISSDETRN
FYKSCYLRENPLSDIDFENLDEVNKKRGLNLCSYICDNRVLKFYKIKDPDFYRVIRKLMS
SDEKFTTAKIDGFKEFQNFRISKEKIPPPAFDETFKKFTFIKMGNKLINNVWKIPTGLDK
IEQEVKKPEGVYEAAQAKWESKISSETSGGEAKDEIIRQWQTLRFLRSRYLFDFDKVNEK
TGVDGLFEEPRKVEALDDSFKEKLLYKINQEHRKKLQDAREYKIGKERKKRALEEEASFP
EREQKIKSQRINSASQTEGDELKSEQTQPKGEISEENTKIKSSEVSSQDPDSGVAGEFAP
Sequence of entity 3 (E, K), FASTA
>7AQO_3 TEX1 isoform 1 (chains E, K)
MSTIGAVDILNQKTITSEVAASVTSKYLQSTFSKGNTSHIEDKRFIHVSSRSHSRFTSTP
ITPNEILSLKFHVSGSSMAYSRMDGSLTVWFIKDASFDKSVEVYIPDCCGSDKLATDLSW
NPTSLNQIAVVSNSSEISLLLINEKSLTASKLRTLSLGSKTKVNTCLYDPLGNWLLAATK
SEKIYLFDVKKDHSSVCSLNISDISQEDNDVVYSLAWSNGGSHIFIGFKSGYLAILKAKH
GILEVCTKIKAHTGPITEIKMDPWGRNFITGSIDGNCYVWNMKSLCCELIINDLNSAVTT
LDVCHLGKILGICTEDEMVYFYDLNSGNLLHSKSLANYKTDPVLKFYPDKSWYIMSGKND
TLSNHFVKNEKNLITYWKDM
Sequence of entity 4 (F, L), FASTA
>7AQO_4 EM14S01-3B_G0007820.mRNA.1.CDS.1 (chains F, L)
GAASDKKGSYVGIHSTGFKDFLLKPELSRAIIDCGFEHPSEVQQHTIPQSIHGTDVLCQA
KSGLGKTAVFVLSTLQQLDPVPGEVAVVVICNARELAYQIRNEYLRFSKYMPDVKTAVFY
GGTPISKDAELLKNKDTAPHIVVATPGRLKALVREKYIDLSHVKNFVIDECDKVLEELDM
RRDVQEIFRATPRDKQVMMFSATLSQEIRPICRRFLQNPLEIFVDDEAKLTLHGLQQYYI
KLEEREKNRKLAQLLDDLEFNQVIIFVKSTTRANELTKLLNASNFPAITVHGHMKQEERI
ARYKAFKDFEKRICVSTDVFGRGIDIERINLAINYDLTNEADQYLHRVGRAGRFGTKGLA
ISFVSSKEDEEVLAKIQERFDVKIAEFPEEGIDPSTYLNN
Sequence of entity 5 (C, J), FASTA
>7AQO_5 BJ4_G0025130.mRNA.1.CDS.1 (chains C, J)
MTKEEGRTYFESLCEEEQSLQESQTHLLNILDILSVLADPRSSDDLLTESLKKLPDLHRE
LINSSIRLRYDKYQTREAQLLEDTKTGRDVAAGVQNPKSISEYYSTFEHLNRDTLRYINL
LKRLSVDLAKQVEVSDPSVTVYEMDKWVPSEKLQGILEQYCAPDTDIRGVDAQIKNYLDQ
IKMARAKFGLENKYSLKERLSTLTKELNHWRKEWDDIEMLMFGDDAHSMKKMIQKIDSLK
SEINAPSESYPVDKEGDIVLE
Sequence of entity 6 (D, I), FASTA
>7AQO_6 THO complex subunit MFT1 (chains D, I)
MPLSQKQIDQVRTKVHYSEVDTPFNKYLDILGKVTKLTGSIINGTLSNDDSKIEKLTEQN
ISQLKESAHLRFLDLQSSIDTKKVADENWETCQQETLAKLENLKDKLPDIKSIHSKLLLR
IGKLQGLYDSVQVINREVEGLSEGRTSLVVTRAEWEKELGTDLVKFLIEKNYLKLVDPGL
KKDSSEERYRIYDDFSKGPKELESINASMKSDIENVRQEVSSYKEKWLRDAEIFGKITSI
FKEELLKRDGLLNEAEGDNIDEDYESDEDEERKERFKRQRSMVEVNTIENVDEKEESDHE
YDDQEDEENEEEDDMEVDVEDIKEDNEVDGESSQQEDNSRQGNNEETDKETGVIEEPDAV
NDAEEADSDHSSRKLGGTTSDFSASSSVEEVK
Primary citation
Structural insights into the nucleic acid remodeling mechanisms of the yeast THO-Sub2 complex. Schuller, S.K., Schuller, J.M., Prabu, J.R. et al. Elife (2020) 9. DOI 10.7554/eLife.61467 · PubMed
Other PDB entries of the same protein (UniProt P53552 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7V2W 3.2 Å, protomer structure from the dimer of yeast THO complex
- 7APX 3.4 Å, yeast THO-Sub2 complex
- 7V2Y 3.4 Å, cryo-EM structure of yeast THO complex with Sub2
- 7LUV 3.7 Å, Cryo-EM structure of the yeast THO-Sub2 complex
Browse structure collections
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