7V2W: THO complex subunit HPR1

protomer structure from the dimer of yeast THO complex. Determined by electron microscopy at 3.2 Å resolution. Released 27 Jul 2022.

Method: Electron microscopy
Resolution: 3.2 Å
Organism: Saccharomyces cerevisiae S288c
Chains: 5
Atoms: 21,619
Mol. weight: 394.73 kDa
Released: 27 Jul 2022

Explore 7V2W in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7V2W contains 142 α-helices and 43 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain F: 37 helices, 0 β-strands

Element	Residues	Length
α-helix	4-20	17
α-helix	33-35	3
α-helix	43-45	3
α-helix	62-77	16
α-helix	85-105	21
α-helix	117-130	14
α-helix	134-140	7
α-helix	143-146	4
α-helix	159-160	2
α-helix	181-190	10
α-helix	198-213	16
α-helix	256-262	7
α-helix	263-267	5
α-helix	271-274	4
α-helix	285-296	12
α-helix	303-312	10
α-helix	314-320	7
α-helix	343-347	5
α-helix	350-353	4
α-helix	357-360	4
α-helix	373-375	3
α-helix	379-385	7
α-helix	397-404	8
α-helix	407-413	7
α-helix	418-427	10
α-helix	442-448	7
α-helix	450-453	4
α-helix	454-459	6
α-helix	460-466	7
α-helix	469-480	12
α-helix	483-491	9
α-helix	506-509	4
α-helix	515-517	3
α-helix	526-531	6
α-helix	539-542	4
α-helix	548-562	15
α-helix	576-583	8

Chain G: 75 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	5-14	10
α-helix	37-47	11
α-helix	58-73	16
α-helix	83-96	14
α-helix	105-116	12
α-helix	122-129	8
α-helix	136-141	6
α-helix	144-146	3
α-helix	155-165	11
β-strand	172	1	1
α-helix	183-192	10
α-helix	203-215	13
β-strand	218	1	1
α-helix	221-230	10
α-helix	240-246	7
α-helix	275-281	7
α-helix	294-300	7
α-helix	303-307	5
α-helix	313-316	4
α-helix	323-340	18
α-helix	392-395	4
α-helix	396-398	3
α-helix	404-414	11
α-helix	418-424	7
α-helix	437-456	20
α-helix	458-462	5
α-helix	464-467	4
α-helix	471-472	2
β-strand	473-475	3	2
β-strand	480-482	3	2
β-strand	484-486	3	3
β-strand	487-491	5	4
β-strand	497-498	2	4
β-strand	502-506	5	4
α-helix	524-527	4
α-helix	539-541	3
α-helix	543-559	17
α-helix	567-579	13
α-helix	582-585	4
α-helix	592-599	8
α-helix	602-604	3
α-helix	607-616	10
α-helix	626-642	17
α-helix	654-664	11
α-helix	666-669	4
α-helix	671-674	4
α-helix	684-690	7
α-helix	691-693	3
α-helix	696-710	15
α-helix	727-741	15
α-helix	751-760	10
α-helix	764-775	12
α-helix	787-790	4
α-helix	798-806	9
α-helix	815-827	13
α-helix	834-844	11
α-helix	854-876	23
α-helix	880-885	6
α-helix	887-888	2
α-helix	900-906	7
α-helix	910-913	4
α-helix	922-927	6
α-helix	942-950	9
α-helix	961-964	4
α-helix	967-973	7
α-helix	980-1012	33
α-helix	1015-1018	4
α-helix	1024-1030	7
α-helix	1031-1035	5
α-helix	1036-1041	6
α-helix	1043-1056	14
α-helix	1059-1071	13
α-helix	1075-1078	4
α-helix	1084-1094	11
α-helix	1098-1105	8
α-helix	1112-1133	22
α-helix	1139-1151	13
α-helix	1152-1154	3
α-helix	1159-1167	9
α-helix	1172-1175	4
α-helix	1182-1193	12
α-helix	1212-1230	19
α-helix	1242-1254	13

Chain H: 5 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	8-11	4
α-helix	17-27	11
β-strand	37-39	3	5
α-helix	44-53	10
β-strand	66-71	6	6
β-strand	77-82	6	6
β-strand	87-91	5	6
β-strand	100-105	6	6
β-strand	117	1	7
β-strand	120	1	7
β-strand	128-131	4	7
β-strand	137-142	6	7
β-strand	149-155	7	7
β-strand	165-168	4	8
β-strand	174-178	5	8
β-strand	183-188	6	8
β-strand	195-200	6	8
α-helix	201-204	4
β-strand	214-217	4	9
β-strand	223-227	5	9
β-strand	232-237	6	9
β-strand	245-250	6	9
β-strand	256-261	6	10
β-strand	267-272	6	10
β-strand	276-281	6	10
β-strand	287-292	6	10
β-strand	298-303	6	11
β-strand	309-314	6	11
β-strand	318-323	6	11
β-strand	329-334	6	11
β-strand	346	1	5
β-strand	353	1	5
β-strand	356	1	12
β-strand	363	1	12
β-strand	364-366	3	5
α-helix	374-388	15

Chain I: 10 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	5-16	12
α-helix	22-41	20
α-helix	61-77	17
α-helix	80-97	18
α-helix	108-141	34
β-strand	146-150	5	13
α-helix	152-155	4
α-helix	161-167	7
β-strand	174-175	2	13
α-helix	188	1
β-strand	189-193	5	13
α-helix	199-234	36
α-helix	238-246	9

Chain J: 15 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	9-12	4
α-helix	15-36	22
α-helix	46-51	6
α-helix	54-78	25
α-helix	82-85	4
α-helix	91-93	3
α-helix	99-102	4
α-helix	104-110	7
α-helix	115-118	4
β-strand	141-143	3	3
α-helix	151-157	7
α-helix	172-177	6
α-helix	202-212	11
α-helix	214-221	8
α-helix	228-231	4
α-helix	233-239	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
THO complex subunit HPR1	F	protein	752	Saccharomyces cerevisiae S288c	P17629 (AlphaFold model)
THO complex subunit 2	G	protein	1597	Saccharomyces cerevisiae S288c	P53552 (AlphaFold model)
Protein TEX1	H	protein	422	Saccharomyces cerevisiae S288c	P53851 (AlphaFold model)
THO complex subunit MFT1	I	protein	392	Saccharomyces cerevisiae S288c	P33441 (AlphaFold model)
THO complex subunit THP2	J	protein	261	Saccharomyces cerevisiae S288c	O13539

Sequence of entity 1 (F), FASTA

>7V2W_1 THO complex subunit HPR1 (chains F)
MSNTEELIQNSIGFLQKTFKALPVSFDSIRHEPLPSSMLHASVLNFEWEPLEKNISAIHD
RDSLIDIILKRFIIDSMTNAIEDEEENNLEKGLLNSCIGLDFVYNSRFNRSNPASWGNTF
FELFSTIIDLLNSPSTFLKFWPYAESRIEWFKMNTSVEPVSLGESNLISYKQPLYEKLRH
WNDILAKLENNDILNTVKHYNMKYKLENFLSELLPINEESNFNRSASISALQESDNEWNR
SARERESNRSSDVIFAADYNFVFYHLIICPIEFAFSDLEYKNDVDRSLSPLLDAILEIEE
NFYSKIKMNNRTRYSLEEALNTEYYANYDVMTPKLPVYMKHSNAMKMDRNEFWANLQNIK
ESDDYTLRPTIMDISLSNTTCLYKQLTQEDDDYYRKQFILQLCFTTNLIRNLISSDETRN
FYKSCYLRENPLSDIDFENLDEVNKKRGLNLCSYICDNRVLKFYKIKDPDFYRVIRKLMS
SDEKFTTAKIDGFKEFQNFRISKEKIPPPAFDETFKKFTFIKMGNKLINNVWKIPTGLDK
IEQEVKKPEGVYEAAQAKWESKISSETSGGEAKDEIIRQWQTLRFLRSRYLFDFDKVNEK
TGVDGLFEEPRKVEALDDSFKEKLLYKINQEHRKKLQDAREYKIGKERKKRALEEEASFP
EREQKIKSQRINSASQTEGDELKSEQTQPKGEISEENTKIKSSEVSSQDPDSGVAGEFAP
QNTTAQLENPKTEDNNAATSNISNGSSTQDMK

Sequence of entity 2 (G), FASTA

>7V2W_2 THO complex subunit 2 (chains G)
MAEQTLLSKLNALSQKVIPPASPSQASILTEEVIRNWPERSKTLCSDFTALESNDEKEDW
LRTLFIELFDFINKNDENSPLKLSDVASFTNELVNHERQVSQASIVGKMFIAVSSTVPNI
NDLTTISLCKLIPSLHEELFKFSWISSKLLNKEQTTLLRHLLKKSKYELKKYNLLVENSV
GYGQLVALLILAYYDPDNFSKVSAYLKEIYHIMGKYSLDSIRTLDVILNVSSQFITEGYK
FFIALLRKSDSWPSSHVANNSNYSSLNEGGNMIAANIISFNLSQYNEEVDKENYERYMDM
CCILLKNGFVNFYSIWDNVKPEMEFLQEYIQNLETELEEESTKGVENPLAMAAALSTENE
TDEDNALVVNDDVNMKDKISEETNADIESKGKQKTQQDILLFGKIKLLERLLIHGCVIPV
IHVLKQYPKVLYVSESLSRYLGRVFEYLLNPLYTSMTSSGESKDMATALMITRIDNGILA
HKPRLIHKYKTHEPFESLELNSSYVFYYSEWNSNLTPFASVNDLFENSHIYLSIIGPYLG
RIPTLLSKISRIGVADIQKNHGSESLHVTIDKWIDYVRKFIFPATSLLQNNPIATSEVYE
LMKFFPFEKRYFIYNEMMTKLSQDILPLKVSFNKAEREAKSILKALSIDTIAKESRRFAK
LISTNPLASLVPAVKQIENYDKVSELVVYTTKYFNDFAYDVLQFVLLLRLTYNRPAVQFD
GVNQAMWVQRLSIFIAGLAKNCPNMDISNIITYILKTLHNGNIIAVSILKELIITVGGIR
DLNEVNMKQLLMLNSGSPLKQYARHLIYDFRDDNSVISSRLTSFFTDQSAISEIILLLYT
LNLKANTQNSHYKILSTRCDEMNTLLWSFIELIKHCLKGKAFEENVLPFVELNNRFHLST
PWTFHIWRDYLDNQLNSNENFSIDELIEGAEFSDVDLTKISKDLFTTFWRLSLYDIHFDK
SLYDERKNALSGENTGHMSNRKKHLIQNQIKDILVTGISHQRAFKKTSEFISEKSNVWNK
DCGEDQIKIFLQNCVVPRVLFSPSDALFSSFFIFMAFRTENLMSILNTCITSNILKTLLF
CCTSSEAGNLGLFFTDVLKKLEKMRLNGDFNDQASRKLYEWHSVITEQVIDLLSEKNYMS
IRNGIEFMKHVTSVFPVVKAHIQLVYTTLEENLINEEREDIKLPSSALIGHLKARLKDAL
ELDEFCTLTEEEAEQKRIREMELEEIKNYETACQNEQKQVALRKQLELNKSQRLQNDPPK
SVASGSAGLNSKDRYTYSRNEPVIPTKPSSSQWSYSKVTRHVDDINHYLATNHLQKAISL
VENDDETRNLRKLSKQNMPIFDFRNSTLEIFERYFRTLIQNPQNPDFAEKIDSLKRYIKN
ISREPYPDTTSSYSEAAAPEYTKRSSRYSGNAGGKDGYGSSNYRGPSNDRSAPKNIKPIS
SYAHKRSELPTRPSKSKTYNDRSRALRPTGPDRGDGFDQRDNRLREEYKKNSSQRSQLRF
PEKPFQEGKDSSKANPYQASSYKRDSPSENEEKPNKRFKKDETIRNKFQTQDYRNTRDSG
AAHRANENQRYNGNRKSNTQALPQGPKGGNYVSRYQR

Sequence of entity 3 (H), FASTA

>7V2W_3 Protein TEX1 (chains H)
MSTIGAVDILNQKTITSEVAASVTSKYLQSTFSKGNTSHIEDKRFIHVSSRSHSRFTSTP
ITPNEILSLKFHVSGSSMAYSRMDGSLTVWFIKDASFDKSVEVYIPDCCGSDKLATDLSW
NPTSLNQIAVVSNSSEISLLLINEKSLTASKLRTLSLGSKTKVNTCLYDPLGNWLLAATK
SEKIYLFDVKKDHSSVCSLNISDISQEDNDVVYSLAWSNGGSHIFIGFKSGYLAILKAKH
GILEVCTKIKAHTGPITEIKMDPWGRNFITGSIDGNCYVWNMKSLCCELIINDLNSAVTT
LDVCHLGKILGICTEDEMVYFYDLNSGNLLHSKSLANYKTDPVLKFYPDKSWYIMSGKND
TLSNHFVKNEKNLITYWKDMFDNTMIEKRRKNNGGGNNHNKRTSKNTDRIGKDRPSRFNS
KK

Sequence of entity 4 (I), FASTA

>7V2W_4 THO complex subunit MFT1 (chains I)
MPLSQKQIDQVRTKVHYSEVDTPFNKYLDILGKVTKLTGSIINGTLSNDDSKIEKLTEQN
ISQLKESAHLRFLDLQSSIDTKKVADENWETCQQETLAKLENLKDKLPDIKSIHSKLLLR
IGKLQGLYDSVQVINREVEGLSEGRTSLVVTRAEWEKELGTDLVKFLIEKNYLKLVDPGL
KKDSSEERYRIYDDFSKGPKELESINASMKSDIENVRQEVSSYKEKWLRDAEIFGKITSI
FKEELLKRDGLLNEAEGDNIDEDYESDEDEERKERFKRQRSMVEVNTIENVDEKEESDHE
YDDQEDEENEEEDDMEVDVEDIKEDNEVDGESSQQEDNSRQGNNEETDKETGVIEEPDAV
NDAEEADSDHSSRKLGGTTSDFSASSSVEEVK

Sequence of entity 5 (J), FASTA

>7V2W_5 THO complex subunit THP2 (chains J)
MTKEEGRTYFESLCEEEQSLQESQTHLLNILDILSVLADPRSSDDLLTESLKKLPDLHRE
LINSSIRLRYDKYQTREAQLLEDTKTGRDVAAGVQNPKSISEYYSTFEHLNRDTLRYINL
LKRLSVDLAKQVEVSDPSVTVYEMDKWVPSEKLQGILEQYCAPDTDIRGVDAQIKNYLDQ
IKMARAKFGLENKYSLKERLSTLTKELNHWRKEWDDIEMLMFGDDAHSMKKMIQKIDSLK
SEINAPSESYPVDKEGDIVLE

Primary citation

Structural and functional insights into R-loop prevention and mRNA export by budding yeast THO-Sub2 complex. Chen, C., Tan, M., Wu, Z. et al. Sci Bull (Beijing) (2021) 66:2347-2352. DOI 10.1016/j.scib.2021.08.004 · PubMed

Other PDB entries of the same protein (UniProt P17629 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7APX 3.4 Å, yeast THO-Sub2 complex
7V2Y 3.4 Å, cryo-EM structure of yeast THO complex with Sub2
7LUV 3.7 Å, Cryo-EM structure of the yeast THO-Sub2 complex
7AQO 4.5 Å, yeast THO-Sub2 complex dimer

Browse structure collections

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