7DSA: Actin capping protein

Crystal structure of actin capping protein in complex with V-1 (space group P62). Determined by X-ray diffraction at 2.8 Å resolution. Released 3 Mar 2021.

Method: X-ray diffraction
Resolution: 2.8 Å
Organisms: Gallus gallus, Homo sapiens
Chains: 3
Atoms: 4,977
Mol. weight: 73.85 kDa
Ligands: MG
Released: 3 Mar 2021

Explore 7DSA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7DSA contains 28 α-helices and 22 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	12-22	11
α-helix	24-25	2
α-helix	29-40	12
α-helix	43-49	7
α-helix	51-60	10
β-strand	63-65	3	1
β-strand	74-76	3	1
β-strand	81	1	2
β-strand	86-89	4	2
β-strand	94-99	6	2
β-strand	104-110	7	2
α-helix	118-135	18
β-strand	139-148	10	3
β-strand	151-164	14	3
α-helix	165-167	3
β-strand	169-182	14	3
β-strand	185-198	14	3
β-strand	203-217	15	3
α-helix	223-249	27
α-helix	250-254	5
α-helix	255-258	4
α-helix	271-273	3

Chain B: 9 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	3-13	11
α-helix	21-31	11
α-helix	33-35	3
α-helix	36-40	5
α-helix	46-47	2
β-strand	48-51	4	4
β-strand	58-61	4	4
β-strand	66-67	2	5
β-strand	70-71	2	5
β-strand	72	1	6
β-strand	79	1	6
α-helix	91-112	22
β-strand	116-123	8	3
β-strand	127-137	11	3
β-strand	144-159	16	3
β-strand	164-181	18	3
β-strand	185-202	18	3
α-helix	209-230	22
α-helix	231-235	5
α-helix	236-243	8

Chain C: 8 helices, 0 β-strands

Element	Residues	Length
α-helix	3-12	10
α-helix	15-23	9
α-helix	30-32	3
α-helix	38-44	7
α-helix	48-56	9
α-helix	71-78	8
α-helix	81-89	9
α-helix	111-116	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
F-actin-capping protein subunit alpha-1	A	protein	286	Gallus gallus	P13127 (AlphaFold model)
F-actin-capping protein subunit beta isoforms 1	B	protein	244	Gallus gallus	P14315 (AlphaFold model)
Myotrophin	C	protein	123	Homo sapiens	P58546 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7DSA_1 F-actin-capping protein subunit alpha-1 (chains A)
MADFEDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMD
QFTPVKIEGYDDQVLITEHGDLGNGRFLDPRNKISFKFDHLRKEASDPQPEDTESALKQW
RDACDSALRAYVKDHYPNGFCTVYGKSIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFT
ITPPTAQVAAVLKIQVHYYEDGNVQLVSHKDIQDSVQVSSDVQTAKEFIKIIENAENEYQ
TAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA

Sequence of entity 2 (B), FASTA

>7DSA_2 F-actin-capping protein subunit beta isoforms 1 (chains B)
MSDQQLDCALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLSSVDQPLKIARDKVVGKDYL
LCDYNRDGDSYRSPWSNKYDPPLEDGAMPSARLRKLEVEANNAFDQYRDLYFEGGVSSVY
LWDLDHGFAGVILIKKAGDGSKKIKGCWDSIHVVEVQEKSSGRTAHYKLTSTVMLWLQTN
KTGSGTMNLGGSLTRQMEKDETVSDSSPHIANIGRLVEDMENKIRSTLNEIYFGKTKDIV
NGLR

Sequence of entity 3 (C), FASTA

>7DSA_3 Myotrophin (chains C)
GPLGSMCDKEFMWALKNGDLDEVKDYVAKGEDVNRTLEGGRKPLHYAADCGQLEILEFLL
LKGADINAPDKHHITPLLSAVYEGHVSCVKLLLSKGADKTVKGPDGLTAFEATDNQAIKA
LLQ

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	2

Primary citation

Structural Insights into the Regulation of Actin Capping Protein by Twinfilin C-terminal Tail. Takeda, S., Koike, R., Fujiwara, I. et al. J Mol Biol (2021) 433:166891-166891. DOI 10.1016/j.jmb.2021.166891 · PubMed

Other PDB entries of the same protein (UniProt P13127 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7DS6 1.69 Å, Crystal structure of actin capping protein in complex with twinflin-1/CD2AP CPI chimera…
3AA0 1.7 Å, Crystal structure of Actin Capping Protein in complex with the Cp-binding motif derived…
7DS4 1.85 Å, Crystal structure of actin capping protein in complex with twinflin-1 C-terminus tail…
3AA1 1.9 Å, Crystal structure of Actin capping protein in complex with the Cp-binding motif derived…
3AA6 1.9 Å, Crystal structure of Actin capping protein in complex with the Cp-binding motif derived…
3AA7 1.9 Å, Crystal structure of Actin capping protein
7DS2 1.95 Å, Crystal structure of actin capping protein in complex with twinflin-1 C-terminus tail
7DS8 1.95 Å, Crystal structure of actin capping protein in complex with twinflin-1/CD2AP CPI chimera…
3LK4 1.99 Å, Crystal structure of CapZ bound to the uncapping motif from CD2AP
9BLI 2.0 Å, Crystal structure of Actin capping protein in complex with a fragment of Legionella…
7DS3 2.09 Å, Crystal structure of actin capping protein in complex with twinflin-2 C-terminus tail
1IZN 2.1 Å, Crystal Structure of Actin Filament Capping Protein CapZ

Browse structure collections

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