7EQ1: GPR114-Gs-scFv16 complex

GPR114-Gs-scFv16 complex. Determined by electron microscopy at 3.3 Å resolution. Released 11 May 2022.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Bos taurus, Homo sapiens, synthetic construct
Chains: 5
Atoms: 8,999
Mol. weight: 147.31 kDa
Released: 11 May 2022

Explore 7EQ1 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7EQ1 contains 35 α-helices and 64 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-39	26
β-strand	41-47	7	1
α-helix	53-59	7
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	234-238	5
β-strand	243-249	7	1
α-helix	266-278	13
β-strand	286-287	2	1
β-strand	290-292	3	1
α-helix	294-303	10
α-helix	308-311	4
α-helix	313-315	3
α-helix	319-321	3
α-helix	332-351	20
β-strand	359	1	1
β-strand	362-364	3	1
α-helix	372-388	17
α-helix	389-391	3

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	6-25	20
α-helix	30-34	5
β-strand	47-51	5	10
β-strand	58-63	6	11
β-strand	69-74	6	11
β-strand	78-83	6	11
β-strand	88-94	7	11
α-helix	95	1
β-strand	100-105	6	12
β-strand	111-116	6	12
β-strand	120-125	6	12
β-strand	134-140	7	12
β-strand	146-151	6	13
β-strand	156-161	6	13
β-strand	165-170	6	13
β-strand	175-181	7	13
β-strand	187-192	6	14
β-strand	198-203	6	14
β-strand	208-212	5	14
β-strand	220-222	3	14
β-strand	229-234	6	15
β-strand	240-245	6	15
β-strand	250-254	5	15
β-strand	259-264	6	15
β-strand	273-278	6	16
β-strand	284-289	6	16
β-strand	294-298	5	16
β-strand	304-308	5	16
β-strand	315-320	6	10
β-strand	327-331	5	10
β-strand	336-339	4	10

Chain R: 15 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	229-232	4
β-strand	239	1	2
α-helix	245-272	28
α-helix	281-298	18
α-helix	300-303	4
α-helix	313-343	31
α-helix	354-363	10
α-helix	371-375	5
β-strand	385	1	3
β-strand	389	1	2
β-strand	402	1	3
α-helix	411-413	3
α-helix	417-441	25
α-helix	457-466	10
α-helix	469-472	4
α-helix	481-482	2
α-helix	483-487	5
α-helix	488-492	5
α-helix	495-519	25

Chain S: 4 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	4
β-strand	10-12	3	5
β-strand	17-25	9	4
β-strand	33-39	7	6
β-strand	45-51	7	6
β-strand	58-60	3	6
α-helix	62-64	3
β-strand	68-73	6	4
β-strand	78-84	7	4
α-helix	88-90	3
β-strand	93-99	7	6
β-strand	111	1	6
β-strand	115-116	2	6
β-strand	117-119	3	5
β-strand	140-142	3	7
β-strand	147-148	2	8
β-strand	155-161	7	7
β-strand	166	1	9
β-strand	172	1	9
β-strand	174-179	6	8
β-strand	186-190	5	8
β-strand	194-195	2	8
β-strand	203-207	5	7
β-strand	211-216	6	7
α-helix	221-223	3
β-strand	225-231	7	8
α-helix	237-238	2
β-strand	243-246	4	8

Chain Y: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	7-23	17
α-helix	30-43	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Gs protein alpha subunit	A	protein	361	Bos taurus	P04896 (AlphaFold model), P63097 (AlphaFold model)
Adhesion G-protein coupled receptor G5	R	protein	302	Homo sapiens	Q8IZF4 (AlphaFold model)
scFv16	S	protein	247	synthetic construct
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	Y	protein	71	Bos taurus	P63212 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Rattus norvegicus	P54311

Sequence of entity 1 (A), FASTA

>7EQ1_1 Gs protein alpha subunit (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 2 (R), FASTA

>7EQ1_2 Adhesion G-protein coupled receptor G5 (chains R)
TYFAVLMQLSPALVPAELLAPLTYISLVGCSISIVASLITVLLHFHFRKQSDSLTRIHMN
LHASVLLLNIAFLLSPAFAMSPVPGSACTALAAALHYALLSCLTWMAIEGFNLYLLLGRV
YNIYIRRYVFKLGVLGWGAPALLVLLSLSVKSSVYGPCTIPVFDSWENGTGFQNMSICWV
RSPVVHSVLVMGYGGLTSLFNLVVLAWALWTLRRLRERADAPSVRACHDTVTVLGLTVLL
GTTWALAFFSFGVFLLPQLFLFTILNSLYGFFLFLWFCSQRCRSEAEAKAQIEAFSSSQT
TQ

Sequence of entity 3 (S), FASTA

>7EQ1_3 scFv16 (chains S)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL

Sequence of entity 4 (Y), FASTA

>7EQ1_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains Y)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (B), FASTA

>7EQ1_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Primary citation

Structural basis for the tethered peptide activation of adhesion GPCRs. Ping, Y.Q., Xiao, P., Yang, F. et al. Nature (2022) 604:763-770. DOI 10.1038/s41586-022-04619-y · PubMed

Other PDB entries of the same protein (UniProt P04896 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1AZS 2.3 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase
1AZT 2.3 Å, Gs-alpha complexed with GTP-gamma-S
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
1CUL 2.4 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase: complex…
1CS4 2.5 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase: complex…
8DCS 2.5 Å, Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with…
7JJO 2.6 Å, Structural Basis of the Activation of Heterotrimeric Gs-protein by Isoproterenol-bound…
8DCR 2.6 Å, Cryo-EM structure of dobutamine-bound beta1-adrenergic receptor in complex with…
7X8R 2.61 Å, Cryo-EM structure of the Boc5-bound hGLP-1R-Gs complex
3C14 2.68 Å, Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex…
7VQX 2.74 Å, Cryo-EM structure of human vasoactive intestinal polypeptide receptor 2 (VIP2R) in…
8X9T 2.75 Å, Identification, structure and agonist design of an androgen membrane receptor

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