7X8R: Boc5-bound hGLP-1R-Gs complex
Cryo-EM structure of the Boc5-bound hGLP-1R-Gs complex. Determined by electron microscopy at 2.61 Å resolution. Released 29 Jun 2022.
- Method
- Electron microscopy
- Resolution
- 2.61 Å
- Organisms
- Bos taurus, Rattus norvegicus, Homo sapiens
- Chains
- 5
- Atoms
- 8,493
- Mol. weight
- 158.64 kDa
- Ligands
- BYI
- Released
- 29 Jun 2022
Explore 7X8R in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7X8R contains 34 α-helices and 48 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 11 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 13-38 | 26 | |
| β-strand | 40-47 | 8 | 1 |
| α-helix | 49-51 | 3 | |
| α-helix | 53-62 | 10 | |
| β-strand | 207-214 | 8 | 1 |
| β-strand | 217-224 | 8 | 1 |
| α-helix | 234-238 | 5 | |
| β-strand | 243-248 | 6 | 1 |
| α-helix | 266-278 | 13 | |
| β-strand | 286-291 | 6 | 1 |
| α-helix | 294-302 | 9 | |
| α-helix | 308-310 | 3 | |
| α-helix | 313-315 | 3 | |
| α-helix | 326-327 | 2 | |
| α-helix | 332-350 | 19 | |
| β-strand | 359-363 | 5 | 1 |
| α-helix | 371-390 | 20 | |
Chain B: 3 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-25 | 22 | |
| α-helix | 30-34 | 5 | |
| β-strand | 47-51 | 5 | 2 |
| β-strand | 58-63 | 6 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 88-94 | 7 | 3 |
| α-helix | 95 | 1 | |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 121-125 | 5 | 4 |
| β-strand | 134-139 | 6 | 4 |
| β-strand | 146-151 | 6 | 5 |
| β-strand | 156-161 | 6 | 5 |
| β-strand | 165-170 | 6 | 5 |
| β-strand | 175-181 | 7 | 5 |
| β-strand | 187-192 | 6 | 6 |
| β-strand | 198-203 | 6 | 6 |
| β-strand | 208-212 | 5 | 6 |
| β-strand | 218-222 | 5 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 250-254 | 5 | 7 |
| β-strand | 259-264 | 6 | 7 |
| β-strand | 273-278 | 6 | 8 |
| β-strand | 284-289 | 6 | 8 |
| β-strand | 294-298 | 5 | 8 |
| β-strand | 303-308 | 6 | 8 |
| β-strand | 315 | 1 | 9 |
| β-strand | 319-320 | 2 | 2 |
| β-strand | 327-330 | 4 | 2 |
| β-strand | 331 | 1 | 9 |
| β-strand | 336-339 | 4 | 2 |
Chain G: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-23 | 15 | |
| α-helix | 30-43 | 14 | |
| α-helix | 53-55 | 3 | |
Chain N: 3 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 10 |
| β-strand | 11 | 1 | 11 |
| β-strand | 17-25 | 9 | 10 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 12 |
| β-strand | 45-51 | 7 | 12 |
| β-strand | 58-60 | 3 | 12 |
| α-helix | 62-64 | 3 | |
| β-strand | 68-73 | 6 | 10 |
| β-strand | 78-84 | 7 | 10 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 12 |
| β-strand | 118 | 1 | 12 |
| β-strand | 122-124 | 3 | 12 |
| β-strand | 125 | 1 | 11 |
Chain R: 14 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 33-51 | 19 | |
| α-helix | 138-169 | 32 | |
| α-helix | 175-207 | 33 | |
| α-helix | 214-222 | 9 | |
| α-helix | 224-255 | 32 | |
| α-helix | 264-274 | 11 | |
| α-helix | 278-291 | 14 | |
| α-helix | 295-297 | 3 | |
| α-helix | 303-337 | 35 | |
| α-helix | 345-360 | 16 | |
| α-helix | 364-367 | 4 | |
| α-helix | 379-399 | 21 | |
| α-helix | 400-404 | 5 | |
| α-helix | 407-422 | 16 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 394 | Bos taurus | P04896 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 345 | Rattus norvegicus | P54311 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 70 | Homo sapiens | P59768 (AlphaFold model) |
| Nanobody 35 | N | protein | 140 | synthetic construct | |
| Glucagon-like peptide 1 receptor | R | protein | 440 | Homo sapiens | P43220 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>7X8R_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
Sequence of entity 2 (B), FASTA
>7X8R_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>7X8R_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFCAIL
Sequence of entity 4 (N), FASTA
>7X8R_4 Nanobody 35 (chains N)
MAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASI
SYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYR
GQGTQVTVSSHHHHHHEPEA
Sequence of entity 5 (R), FASTA
>7X8R_5 Glucagon-like peptide 1 receptor (chains R)
RPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNV
SCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLF
LYIIYTVGYALSFSALVIASAILLGFRHLHCTRNYIHLNLFASFILRALSVFIKDAALKW
MYSTAAQQHQWDGLLSYQDSLSCRLVFLLMQYCVAANYYWLLVEGVYLYTLLAFSVFSEQ
WIFRLYVSIGWGVPLLFVVPWGIVKYLYEDEGCWTRNSNMNYWLIIRLPILFAIGVNFLI
FVRVICIVVSKLKANLMCKTDIKCRLAKSTLTLIPLLGTHEVIFAFVMDEHARGTLRFIK
LFTELSFTSFQGLMVAILYCFVNNEVQLEFRKSWERWRLEHLHIQRDSSMKPLKCPTSSL
SSGATAGSSMYTATCQASCS
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| BYI | 2,4-bis(3-methoxy-4-thiophen-2-ylcarbonyloxy-phenyl)-1,3-bis[[4-[(2-methylpropa… | C54 H52 N4 O16 S2 | 1 |
Primary citation
Structural basis of peptidomimetic agonism revealed by small- molecule GLP-1R agonists Boc5 and WB4-24. Cong, Z., Zhou, Q., Li, Y. et al. Proc Natl Acad Sci U S A (2022) 119:e2200155119-e2200155119. DOI 10.1073/pnas.2200155119 · PubMed
Other PDB entries of the same protein (UniProt P04896 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 1AZS 2.3 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase
- 1AZT 2.3 Å, Gs-alpha complexed with GTP-gamma-S
- 9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
- 1CUL 2.4 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase: complex…
- 1CS4 2.5 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase: complex…
- 8DCS 2.5 Å, Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with…
- 7JJO 2.6 Å, Structural Basis of the Activation of Heterotrimeric Gs-protein by Isoproterenol-bound…
- 8DCR 2.6 Å, Cryo-EM structure of dobutamine-bound beta1-adrenergic receptor in complex with…
- 3C14 2.68 Å, Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex…
- 7VQX 2.74 Å, Cryo-EM structure of human vasoactive intestinal polypeptide receptor 2 (VIP2R) in…
- 8X9T 2.75 Å, Identification, structure and agonist design of an androgen membrane receptor
- 9IJD 2.76 Å, Carazolol-activated human beta3 adrenergic receptor
Browse structure collections
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