8X9T: Gs protein alpha subunit

Identification, structure and agonist design of an androgen membrane receptor. Determined by electron microscopy at 2.75 Å resolution. Released 12 Feb 2025.

Method: Electron microscopy
Resolution: 2.75 Å
Organisms: Bos taurus, Rattus norvegicus, Homo sapiens
Chains: 5
Atoms: 8,260
Mol. weight: 182.42 kDa
Ligands: YNH
Released: 12 Feb 2025

Explore 8X9T in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8X9T contains 28 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	14-37	24
β-strand	41-47	7	1
α-helix	53-58	6
β-strand	207-210	4	1
β-strand	213-214	2	2
β-strand	217-218	2	2
β-strand	219-224	6	1
α-helix	235-238	4
β-strand	243-249	7	1
α-helix	266-273	8
β-strand	286-292	7	1
α-helix	294-301	8
α-helix	308-311	4
α-helix	332-350	19
β-strand	359-363	5	1
α-helix	371-390	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-52	6	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	89-94	6	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-151	6	6
β-strand	157-161	5	6
β-strand	165-168	4	6
β-strand	178-181	4	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	249-254	6	8
β-strand	259-265	7	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-308	5	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	335-339	5	3

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-22	15
α-helix	30-43	14
α-helix	45-47	3

Chain R: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	569-591	23
α-helix	600-614	15
α-helix	617-623	7
α-helix	630-661	32
α-helix	672-678	7
α-helix	682-692	11
α-helix	714-720	7
α-helix	726-743	18
α-helix	754-768	15
α-helix	788-806	19
α-helix	813-826	14

Chain S: 3 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	11-12	2	11
β-strand	18-25	8	10
β-strand	33-39	7	12
β-strand	45-51	7	12
β-strand	58-60	3	12
β-strand	68-73	6	10
β-strand	78-83	6	10
α-helix	88-90	3
β-strand	92-94	3	13
β-strand	95-99	5	12
β-strand	110-111	2	12
β-strand	115-117	3	13
β-strand	118-119	2	11
β-strand	128-129	2	14
β-strand	134-136	3	15
β-strand	143-147	5	16
β-strand	148-149	2	14
β-strand	154	1	17
β-strand	160	1	17
β-strand	162-167	6	15
β-strand	174-177	4	15
β-strand	183	1	15
α-helix	184	1
β-strand	191-196	6	16
β-strand	199-204	6	16
β-strand	213-219	7	15
α-helix	225	1
β-strand	226	1	12
β-strand	231-234	4	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Gs protein alpha subunit	A	protein	361	Bos taurus	P04896 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	344	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
Adhesion G-protein coupled receptor D1	R	protein	598	Homo sapiens	Q6QNK2 (AlphaFold model)
scFv16	S	protein	267	synthetic construct

Sequence of entity 1 (A), FASTA

>8X9T_1 Gs protein alpha subunit (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 2 (B), FASTA

>8X9T_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
GSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLA
KIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGG
LDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQ
TTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP
NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNV
WDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>8X9T_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (R), FASTA

>8X9T_4 Adhesion G-protein coupled receptor D1 (chains R)
HPIITNLTEERKTFQSPGVILSYLQNVSLSLPSKSLSEQTALNLTKTFLKAVGEILLLPG
WIALSEDSAVVLSLIDTIDTVMGHVSSNLHGSTPQVTVEGSSAMAEFSVAKILPKTVNSS
HYRFPAHGQSFIQIPHEAFHRHAWSTVVGLLYHSMHYYLNNIWPAHTKIAEAMHHQDCLL
FATSHLISLEVSPPPTLSQNLSGSPLITVHLKHRLTRKQHSEATNSSNRVFVYCAFLDFS
SGEGVWSNHGCALTRGNLTYSVCRCTHLTNFAILMQVVPLELARGHQVALSSISYVGCSL
SVLCLVATLVTFAVLSSVSTIRNQRYHIHANLSFAVLVAQVLLLISFRLEPGTTPCQVMA
VLLHYFFLSAFAWMLVEGLHLYSMVIKVFGSEDSKHRYYYGMGWGFPLLICIISLSFAMD
SYGTSNNCWLSLASGAIWAFVAPALFVIVVNIGILIAVTRVISQISADNYKIHGDPSAFK
LTAKAVAVLLPILGTSWVFGVLAVNGCAVVFQYMFATLNSLQGLFIFLFHCLLNSEVRAA
FKHKTKVWSLTSSSARTSNAKPFHSDLMNGTRPGMASTKLSPWDKSSHSAHRVDLSAV

Sequence of entity 5 (S), FASTA

>8X9T_5 scFv16 (chains S)
MVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
AGGGGSGGGGSGGGGSSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQ
RPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTF
GAGTKLELLEENLYFQGASHHHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
YNH	methyl 3-[2-(2,4-dimethylphenoxy)ethanoylamino]-4-(4-ethylpiperazin-1-yl)benzoa…	C24 H31 N3 O4	1

Primary citation

Identification, structure, and agonist design of an androgen membrane receptor. Yang, Z., Ping, Y.Q., Wang, M.W. et al. Cell (2025) 188:1589. DOI 10.1016/j.cell.2025.01.006 · PubMed

Other PDB entries of the same protein (UniProt P04896 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1AZS 2.3 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase
1AZT 2.3 Å, Gs-alpha complexed with GTP-gamma-S
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
1CUL 2.4 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase: complex…
1CS4 2.5 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase: complex…
8DCS 2.5 Å, Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with…
7JJO 2.6 Å, Structural Basis of the Activation of Heterotrimeric Gs-protein by Isoproterenol-bound…
8DCR 2.6 Å, Cryo-EM structure of dobutamine-bound beta1-adrenergic receptor in complex with…
7X8R 2.61 Å, Cryo-EM structure of the Boc5-bound hGLP-1R-Gs complex
3C14 2.68 Å, Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex…
7VQX 2.74 Å, Cryo-EM structure of human vasoactive intestinal polypeptide receptor 2 (VIP2R) in…
9IJD 2.76 Å, Carazolol-activated human beta3 adrenergic receptor

Browse structure collections

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