7F1S: Apo chemokine receptor CCR5

Cryo-EM structure of the apo chemokine receptor CCR5 in complex with Gi. Determined by electron microscopy at 2.8 Å resolution. Released 14 Jul 2021.

Method: Electron microscopy
Resolution: 2.8 Å
Organism: Homo sapiens
Chains: 4
Atoms: 6,209
Mol. weight: 127.75 kDa
Released: 14 Jul 2021

Explore 7F1S in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7F1S contains 35 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-39	6	2
α-helix	49-51	3
β-strand	185-190	6	2
β-strand	195-200	6	2
α-helix	208-210	3
α-helix	213-215	3
β-strand	220-226	7	2
α-helix	242-254	13
β-strand	263-269	7	2
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-308	13
β-strand	319-323	5	2
α-helix	328-351	24

Chain B: 6 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	23-25	3
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	103-105	3	5
β-strand	111-114	4	5
β-strand	122-125	4	5
α-helix	129-131	3
β-strand	134-137	4	5
α-helix	145	1
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	176-180	5	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	221-223	3	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	249-254	6	8
β-strand	260-265	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	307-308	2	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain C: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	30-43	14

Chain R: 19 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	34-54	21
α-helix	55-59	5
α-helix	65-80	16
α-helix	82-86	5
α-helix	99-131	33
α-helix	133-139	7
α-helix	142-165	24
β-strand	168-171	4	1
β-strand	177-179	3	1
α-helix	187-199	13
α-helix	200-204	5
α-helix	205-219	15
α-helix	227-247	21
α-helix	249-259	11
α-helix	261-265	5
α-helix	266-268	3
α-helix	271-276	6
α-helix	278-287	10
α-helix	293	1
α-helix	294-298	5
α-helix	304-312	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
C-C chemokine receptor type 5	R	protein	367	Homo sapiens	P51681 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (R), FASTA

>7F1S_1 C-C chemokine receptor type 5 (chains R)
GAPDYQVSSPIYDINYYTSEPCQKINVKQIAARLLPPLYSLVFIFGFVGNMLVILILINC
KRLKSMTDIYLLNLAISDLFFLLTVPFWAHYAAAQWDFGNTMCQLLTGLYFIGFFSGIFF
IILLTIDRYLAVVHAVFALKARTVTFGVVTSVITWVVAVFASLPNIIFTRSQKEGLHYTC
SSHFPYSQYQFWKNFQTLKIVILGLVLPLLVMVICYSGILKTLLRCRNEKKRHRAVRLIF
TIMIVYFLFWAPYNIVLLLNTFQEFFGLNNCSSSNRLDQAMQVTETLGMTHCCINPIIYA
FVGEKFRNYLLVFFQKHIAKRLEVLFQGPGSWSHPQFEKGSGAGASAGSWSHPQFEKGSD
YKDDDDK

Sequence of entity 2 (A), FASTA

>7F1S_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKCTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVTAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (B), FASTA

>7F1S_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>7F1S_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

Structural basis for chemokine recognition and receptor activation of chemokine receptor CCR5. Zhang, H., Chen, K., Tan, Q. et al. Nat Commun (2021) 12:4151-4151. DOI 10.1038/s41467-021-24438-5 · PubMed

Other PDB entries of the same protein (UniProt P51681 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5YD3 1.35 Å, Crystal structure of the scFv antibody 4B08 with epitope peptide
5YD4 1.35 Å, Crystal structure of the scFv antibody 4B08 with epitope peptide (mutation T6A)
5YY4 1.59 Å, Crystal structure of the scFv antibody 4B08 with sulfated epitope peptide
9J8A 1.75 Å, Structure of antibody BA8 in complex with sulfated peptide from CCR5
5YD5 1.96 Å, Crystal structure of the scFv antibody 4B08 with epitope peptide (mutation N3A)
5UIW 2.2 Å, Crystal Structure of CC Chemokine Receptor 5 (CCR5) in complex with high potency HIV…
7F1T 2.6 Å, Crystal structure of the human chemokine receptor CCR5 in complex with MIP-1a
4MBS 2.71 Å, Crystal Structure of the CCR5 Chemokine Receptor
6AKX 2.8 Å, The Crystal structure of Human Chemokine Receptor CCR5 in complex with compound 21
6AKY 2.8 Å, The Crystal structure of Human Chemokine Receptor CCR5 in complex with compound 34
7F1Q 2.9 Å, Cryo-EM structure of the chemokine receptor CCR5 in complex with MIP-1a and Gi
7F1R 3.0 Å, Cryo-EM structure of the chemokine receptor CCR5 in complex with RANTES and Gi

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