7F1Q: Chemokine receptor CCR5

Cryo-EM structure of the chemokine receptor CCR5 in complex with MIP-1a and Gi. Determined by electron microscopy at 2.9 Å resolution. Released 14 Jul 2021.

Method: Electron microscopy
Resolution: 2.9 Å
Organism: Homo sapiens
Chains: 4
Atoms: 7,123
Mol. weight: 136.97 kDa
Released: 14 Jul 2021

Explore 7F1Q in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7F1Q contains 29 α-helices and 41 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-39	6	4
α-helix	47-50	4
β-strand	184-190	7	4
β-strand	195-201	7	4
α-helix	208-210	3
β-strand	220-226	7	4
α-helix	242-254	13
β-strand	263-269	7	4
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-309	14
β-strand	319-323	5	4
α-helix	328-351	24

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	5
β-strand	58-62	5	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	88-94	7	6
β-strand	103-105	3	7
β-strand	111-114	4	7
β-strand	121-125	5	7
α-helix	129-131	3
β-strand	135-139	5	7
β-strand	148-151	4	8
β-strand	156-161	6	8
β-strand	165-170	6	8
β-strand	176-181	6	8
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	207-212	6	9
β-strand	218-222	5	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	260-264	5	10
β-strand	273-278	6	11
β-strand	284-289	6	11
β-strand	293-298	6	11
β-strand	304-309	6	11
β-strand	315-320	6	5
β-strand	327-331	5	5
β-strand	336-339	4	5

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	30-43	14
α-helix	56-58	3

Chain R: 16 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	3-5	3
β-strand	9-10	2	1
β-strand	26-29	4	2
β-strand	39-42	4	2
β-strand	48-51	4	2
α-helix	57-68	12
β-strand	116-117	2	1
α-helix	119-128	10
α-helix	130-150	21
α-helix	151-155	5
α-helix	160-187	28
α-helix	194-227	34
α-helix	229-235	7
α-helix	238-261	24
β-strand	264-268	5	3
β-strand	271-275	5	3
α-helix	283-295	13
α-helix	296-300	5
α-helix	301-316	16
α-helix	317-319	3
α-helix	323-355	33
α-helix	365-393	29
α-helix	398-408	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
C-C motif chemokine 3,C-C chemokine receptor type 5	R	protein	457	Homo sapiens	P10147 (AlphaFold model), P51681 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768

Sequence of entity 1 (R), FASTA

>7F1Q_1 C-C motif chemokine 3,C-C chemokine receptor type 5 (chains R)
SLAADTPTACCFSYCSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQK
YVSDLELSAGSGSGSGSGSGSGSGSGSGSGSGSDYQVSSPIYDINYYCSEPCQKINVKQI
AARLLPPLYSLVFIFGFVGNMLVILILINCKRLKSMTDIYLLNLAISDLFFLLTVPFWAH
YAAAQWDFGNTMCQLLTGLYFIGFFSGIFFIILLTIDRYLAVVHAVFALKARTVTFGVVT
SVITWVVAVFASLPNIIFTRSQKEGLHYTCSSHFPYSQYQFWKNFQTLKIVILGLVLPLL
VMVICYSGILKTLLRCRNEKKRHRAVRLIFTIMIVYFLFWAPYNIVLLLNTFQEFFGLNN
CSSSNRLDQAMQVTETLGMTHCCINPIIYAFVGEKFRNYLLVFFQKHIAKRLEVLFQGPG
SWSHPQFEKGSGAGASAGSWSHPQFEKGSDYKDDDDK

Sequence of entity 2 (A), FASTA

>7F1Q_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKCTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVTAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (B), FASTA

>7F1Q_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>7F1Q_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

Structural basis for chemokine recognition and receptor activation of chemokine receptor CCR5. Zhang, H., Chen, K., Tan, Q. et al. Nat Commun (2021) 12:4151-4151. DOI 10.1038/s41467-021-24438-5 · PubMed

Other PDB entries of the same protein (UniProt P10147 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3FPU 1.76 Å, The crystallographic structure of the Complex between Evasin-1 and CCL3
2X6G 2.18 Å, X-ray Structure of Macrophage Inflammatory Protein-1 alpha (D27A)
5COR 2.55 Å, X-ray structure of macrophage inflammatory protein-1 alpha (CCL3) N-terminal-switch…
4RA8 2.6 Å, Structure analysis of the Mip1a P8A mutant
7F1T 2.6 Å, Crystal structure of the human chemokine receptor CCR5 in complex with MIP-1a
2X69 2.65 Å, X-ray Structure of Macrophage Inflammatory Protein-1 alpha polymer
3KBX 2.65 Å, Human macrophage inflammatory protein-1 alpha L3M_V63M
4ZKB 2.9 Å, The chemokine binding protein of orf virus complexed with CCL3
3H44 3.0 Å, Crystal Structure of Insulin Degrading Enzyme in Complex with macrophage inflammatory…
5D65 3.1 Å, X-ray structure of macrophage inflammatory protein-1 alpha (CCL3) with heparin complex
1B50 NMR structure of human mip-1A D26A, 10 structures
1B53 NMR structure of human mip-1A D26A, minimized average structure

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