7F6H: Bradykinin receptor BK2R

Cryo-EM structure of human bradykinin receptor BK2R in complex Gq proteins and bradykinin. Determined by electron microscopy at 2.9 Å resolution. Released 5 Jan 2022.

Method
Electron microscopy
Resolution
2.9 Å
Organism
Homo sapiens
Chains
5
Atoms
7,342
Mol. weight
180.16 kDa
Ligands
CLR
Released
5 Jan 2022

Explore 7F6H in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7F6H contains 32 α-helices and 40 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 4 β-strands

ElementResiduesLengthSheet
α-helix48-503
α-helix52-8433
α-helix91-11828
α-helix127-15933
α-helix171-19525
β-strand19611
β-strand200-20122
β-strand208-20922
β-strand21311
α-helix218-2269
α-helix227-2348
α-helix235-25319
α-helix266-29833
α-helix304-31714
α-helix320-33213
α-helix337-35014
Chain B: 12 helices, 8 β-strands
ElementResiduesLengthSheet
α-helix15-3622
β-strand39-4683
α-helix52-6211
β-strand190-19563
β-strand200-20563
α-helix213-2164
α-helix217-2204
β-strand225-23173
α-helix232-2365
α-helix2371
β-strand23814
α-helix2391
β-strand24614
α-helix247-26014
α-helix262-2643
β-strand268-27473
α-helix276-2827
α-helix304-31411
β-strand324-32853
α-helix336-35520
Chain C: 4 helices, 28 β-strands
ElementResiduesLengthSheet
α-helix12-2413
α-helix30-334
β-strand47-5265
β-strand58-6366
β-strand69-7466
β-strand78-8366
β-strand88-9476
α-helix951
β-strand100-10567
β-strand111-11667
β-strand121-12557
β-strand134-13967
β-strand146-15168
β-strand156-16168
β-strand166-17058
β-strand176-18058
β-strand187-19269
β-strand198-20369
β-strand207-21269
β-strand218-22369
β-strand229-234610
β-strand240-245610
β-strand250-254510
β-strand259-264610
α-helix2721
β-strand273-278611
β-strand284-289611
β-strand294-298511
β-strand304-308511
β-strand315-32065
β-strand327-33155
β-strand335-33955
Chain D: 4 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix16-238
α-helix30-4314
α-helix54-552
α-helix56-583

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Bradykinin receptor BK2RAprotein770Homo sapiensP30411 (AlphaFold model)
BradykininLprotein9Homo sapiensP01042 (AlphaFold model)
Guanine nucleotide-binding protein G(q) subunit alphaBprotein369Homo sapiensP50148 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1Cprotein354Homo sapiensP62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2Dprotein81Homo sapiensP59768
Sequence of entity 1 (A), FASTA
>7F6H_1 Bradykinin receptor BK2R (chains A)
MKTIIALSYIFCLVFADYKDDDDKHHHHHHHHHHENLYFQGKIEEGKLVIWINGDKGYNG
LAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEI
TPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKEL
KAKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSAVNYGVTVLPTFKGQPSKPFV
GVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAA
TMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTLNVTLQGPTLNGT
FAQSKCPQVEWLGWLNTIQPPFLWVLFVLATLENIFVLSVFCLHKSSCTVAEIYLGNLAA
ADLILACGLPFWAITISNNFDWLFGETLCRVVNAIISMNLYSSICFLMLVSIDRYLALVK
TMSMGRMRGVRWAKLYSLVIWGCTLLLSSPMLVFRTMKEYSDEGHNVTACVISYPSLIWE
VFTNMLLNVVGFLLPLSVITFCTMQIMQVLRNNEMQKFKEIQTERRATVLVLVVLLLFII
CWLPFQISTFLDTLHRLGILSSCQDERIIDVITQIASFMAYSNSCLNPLVYVIVGKRFRK
KSWEVYQGVCQKGGCRSEPIQMENSMGTLRTSISVERQIHKLQDWAGSRQ
Sequence of entity 2 (L), FASTA
>7F6H_2 Bradykinin (chains L)
RPPGFSPFR
Sequence of entity 3 (B), FASTA
>7F6H_3 Guanine nucleotide-binding protein G(q) subunit alpha (chains B)
MHHHHHHHHHHTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGES
GKSTFIKQMRIIHGSGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHA
QLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADP
AYLPTQQDVLRVQVPTTGIIEYPFDLQSVIFRMVDVGGLRSERRKWIHCFENVTSIMFLV
ALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVD
YFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQ
LNLKEYNLV
Sequence of entity 4 (C), FASTA
>7F6H_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMR
TRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYA
PSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTC
ALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHE
SDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLL
AGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 5 (D), FASTA
>7F6H_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MHHHHHHHHHHASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPL
LTPVPASENPFREKKFFCAIL

Ligands and cofactors

IDNameFormulaCopies
CLRCholesterolC27 H46 O2

Primary citation

Cryo-EM structures of human bradykinin receptor-G q proteins complexes. Shen, J., Zhang, D., Fu, Y. et al. Nat Commun (2022) 13:714-714. DOI 10.1038/s41467-022-28399-1 · PubMed

Other PDB entries of the same protein (UniProt P30411 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 7F6H directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.