Structure of thermostabilised human NTCP in complex with Megabody 91. Determined by electron microscopy at 3.3 Å resolution. Released 18 May 2022.
Explore 7PQQ in 3D Show helices and sheets RCSB PDB PDBe
7PQQ contains 22 α-helices and 18 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 17 | 1 | |
| α-helix | 24-29 | 6 | |
| α-helix | 31-42 | 12 | |
| α-helix | 43-45 | 3 | |
| α-helix | 48-50 | 3 | |
| α-helix | 51-56 | 6 | |
| α-helix | 59-69 | 11 | |
| α-helix | 72-83 | 12 | |
| α-helix | 89-98 | 10 | |
| β-strand | 101 | 1 | 1 |
| α-helix | 106-110 | 5 | |
| α-helix | 116-121 | 6 | |
| α-helix | 134-137 | 4 | |
| β-strand | 146 | 1 | 2 |
| α-helix | 157-179 | 23 | |
| α-helix | 182-209 | 28 | |
| α-helix | 214-217 | 4 | |
| α-helix | 220-242 | 23 | |
| α-helix | 251-258 | 8 | |
| β-strand | 261 | 1 | 1 |
| α-helix | 266-273 | 8 | |
| α-helix | 276-279 | 4 | |
| α-helix | 280-283 | 4 | |
| α-helix | 284-306 | 23 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 4 | 1 | 3 |
| β-strand | 5-6 | 2 | 4 |
| β-strand | 11 | 1 | 5 |
| β-strand | 790-794 | 5 | 4 |
| β-strand | 803-809 | 7 | 6 |
| β-strand | 815-822 | 8 | 6 |
| β-strand | 828 | 1 | 6 |
| β-strand | 839-842 | 4 | 4 |
| β-strand | 848-852 | 5 | 4 |
| β-strand | 862-869 | 8 | 6 |
| β-strand | 872 | 1 | 2 |
| α-helix | 880-882 | 3 | |
| β-strand | 885-886 | 2 | 6 |
| β-strand | 887 | 1 | 3 |
| β-strand | 890-892 | 3 | 6 |
| β-strand | 893 | 1 | 5 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Sodium/bile acid cotransporter | A | protein | 333 | Homo sapiens | Q14973 (AlphaFold model) |
| Anti-RON nanobody,Megabody 91,Glucosidase YgjK | B | protein | 906 | Lama glama | P42592 (AlphaFold model) |
>7PQQ_1 Sodium/bile acid cotransporter (chains A) MAHTASAPFTFTLPPNFGKRPTDLALSVILVVMLFIIMLSLGCTMEFSKIKAHLWKPKGL AIALVAQYGIMPLTAFVLGKVFRLNNIEALAILICGCSPGGNLSNIFSLAMKGDMNLSIV MTTCSTFLALGMMPLLLYIYSRGIYDGDLKDKVPYKGIVISLVLVLIPCTIGIVLKSKRP QYMRYVIKGGMIIILLCSVAVTVLSAINVGKSIMFAMTPHLIATSSLMPFIGFLLGYVLS ALFCLNGRCRRTVSMETGCQNVQLCSTILNVAFPPEVIGPLFFFPLLYMIFQLGEGLLLI AIFWCYEKFKTPKDKTKMIYTAATTEELEVLFQ
>7PQQ_2 Anti-RON nanobody,Megabody 91,Glucosidase YgjK (chains B) QVQLVESGGGLVKEETQSGLNNYARVVEKGQYDSLEIPAQVAASWESGRDDAAVFGFIDK EQLDKYVANGGKRSDWTVKFAENRSQDGTLLGYSLLQESVDQASYMYSDNHYLAEMATIL GKPEEAKRYRQLAQQLADYINTCMFDPTTQFYYDVRIEDKPLANGCAGKPIVERGKGPEG WSPLFNGAATQANADAVVKVMLDPKEFNTFVPLGTAALTNPAFGADIYWRGRVWVDQFWF GLKGMERYGYRDDALKLADTFFRHAKGLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYM LYNDFFRKQASGGGSGGGGSGGGGSGNADNYKNVINRTGAPQYMKDYDYDDHQRFNPFFD LGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQDGKKVDFTLEAYSIPG ALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDGELLEKLEAKEGKPLSDK TIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEYQVHKSLPVQTEINGNRFTS KAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAFYLTASQQRWEEYLKKGLTNPD ATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVTGRWFSGNQTWPWDTWKQAFAMAH FNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDLIAWNLSPERGGDGGNWNERNTKPSL AAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWLRNRDHNGNGVPEYGATRDKAHNTESGE MLFTVKKSLRLSCAASTNLRSYAMAWFRQAPGKEREFVSFINWNYGNTRYADSVKGRFTI SRDNAKITVYLQMNSLKPEDTAVYYCAAATIGRLAGIDSTTLYDYWGQGTQVTVSSHHHH HHEPEA
Structural basis of sodium-dependent bile salt uptake into the liver. Goutam, K., Ielasi, F.S., Pardon, E. et al. Nature (2022) 606:1015-1020. DOI 10.1038/s41586-022-04723-z · PubMed
Other PDB entries of the same protein (UniProt Q14973 (AlphaFold model), which also has an AlphaFold model), best resolution first:
MolViewer shows 7PQQ directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.