7QSA: Protein scribble homolog

Structural basis on the interaction of Scribble PDZ domains with the Tick Born encephalitis virus (TBEV) NS5 protein. Determined by X-ray diffraction at 2.02 Å resolution. Released 31 Aug 2022.

Method: X-ray diffraction
Resolution: 2.02 Å
Organisms: Homo sapiens, Tick-borne encephalitis virus
Chains: 3
Atoms: 1,313
Mol. weight: 20.45 kDa
Released: 31 Aug 2022

Explore 7QSA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7QSA contains 5 α-helices and 13 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	12-17	6	1
β-strand	26-30	5	1
β-strand	46-51	6	1
α-helix	56-60	5
α-helix	66	1
β-strand	67-71	5	1
β-strand	74-75	2	1
α-helix	81-89	9
β-strand	95-100	6	1

Chain B: 2 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	12-17	6	1
β-strand	26-30	5	1
β-strand	46-51	6	1
α-helix	66	1
β-strand	67-71	5	1
β-strand	74-75	2	1
α-helix	81-88	8
β-strand	95-100	6	1

Chain C: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	54-57	4	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein scribble homolog	A, B	protein	92	Homo sapiens	Q14160 (AlphaFold model)
RNA-directed RNA polymerase NS5	C	protein	8	Tick-borne encephalitis virus	Q01299

Sequence of entity 1 (A, B), FASTA

>7QSA_1 Protein scribble homolog (chains A, B)
SVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQEPGVFISKVLPRGLAARSGLRVGDRILA
VNGQDVRDATHQEAVSALLRPCLELSLLVRRD

Sequence of entity 2 (C), FASTA

>7QSA_2 RNA-directed RNA polymerase NS5 (chains C)
LRLESSII

Primary citation

Molecular basis of Tick Born encephalitis virus NS5 mediated subversion of apico-basal cell polarity signalling. Javorsky, A., Humbert, P.O., Kvansakul, M. Biochem J (2022) 479:1303-1315. DOI 10.1042/BCJ20220037 · PubMed

Other PDB entries of the same protein (UniProt Q14160 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6MYE 1.1 Å, Crystal structure of human Scribble PDZ1 domain in complex with internal PDZ binding…
6EEY 1.15 Å, Crystal structure of human Scribble PDZ4 R1110G Mutant
2W4F 1.3 Å, Crystal structure of the first PDZ domain of human SCRIB1
6MS1 1.35 Å, Crystal structure of the human Scribble PDZ1 domain bound to the PDZ-binding motif of APC
6MYF 1.6 Å, Crystal structure of free human Scribble PDZ1 domain
5VWI 1.75 Å, Crystal structure of human Scribble PDZ1:Beta-PIX complex
7QRS 1.77 Å, Structural insight into the Scribble PDZ domains interaction with the oncogenic Human…
7QS9 1.8 Å, Structural basis on the interaction of Scribble PDZ domains with the Tick Born…
7QSB 1.84 Å, Structural basis on the interaction of Scribble PDZ domains with the Tick Born…
7QS8 1.85 Å, Structural insight into the Scribble PDZ domains interaction with the oncogenic Human…
7QRT 1.9 Å, Structural insight into the Scribble PDZ domains interaction with the oncogenic Human…
7QTP 1.9 Å, Structural biology of the NS1 avian influenza protein subversion on the Scribble cell…

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