7RA3: PDB entry 7RA3

cryo-EM of human Gastric inhibitory polypeptide receptor GIPR bound to GIP. Determined by electron microscopy at 3.24 Å resolution. Released 13 Apr 2022.

Method: Electron microscopy
Resolution: 3.24 Å
Organisms: Homo sapiens, Mus musculus, Lama glama
Chains: 7
Atoms: 10,802
Mol. weight: 196.87 kDa
Released: 13 Apr 2022

Explore 7RA3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7RA3 contains 44 α-helices and 75 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	17-37	21
β-strand	40-46	7	1
α-helix	54-58	5
β-strand	210-213	4	1
β-strand	218-222	5	1
α-helix	234-239	6
β-strand	243-248	6	1
α-helix	265-277	13
β-strand	286-291	6	1
α-helix	294-303	10
α-helix	313-316	4
α-helix	319-321	3
α-helix	326-327	2
α-helix	332-351	20
β-strand	359-362	4	1
α-helix	372-390	19

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-24	20
α-helix	31-34	4
α-helix	38-39	2
β-strand	47-52	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	102-105	4	4
β-strand	111-115	5	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-149	4	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	175-181	7	5
β-strand	187-192	6	6
β-strand	199-203	5	6
β-strand	208-211	4	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	260-264	5	7
α-helix	272-273	2
β-strand	275-278	4	8
β-strand	284-288	5	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain E: 5 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	10-12	3	10
β-strand	17-25	9	9
α-helix	29-31	3
β-strand	33-39	7	10
β-strand	45-51	7	10
α-helix	53-55	3
β-strand	58-60	3	10
α-helix	62-64	3
β-strand	68-73	6	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-99	8	10
β-strand	110	1	11
β-strand	111	1	10
β-strand	115-119	5	10
β-strand	128-129	2	12
β-strand	134-135	2	13
β-strand	142-147	6	14
β-strand	148-149	2	12
β-strand	154	1	15
β-strand	160	1	15
β-strand	162-167	6	11
β-strand	174-178	5	11
β-strand	182-183	2	11
β-strand	191-196	6	14
β-strand	199-205	7	14
β-strand	214-219	6	11
α-helix	225	1
β-strand	226-227	2	11
β-strand	232-233	2	13

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-44	15
α-helix	45-47	3

Chain N: 5 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	16
β-strand	17-25	9	16
α-helix	29-31	3
β-strand	34-39	6	17
β-strand	45-51	7	17
β-strand	59-60	2	17
α-helix	62-64	3
β-strand	68-73	6	16
β-strand	78-84	7	16
α-helix	88-90	3
β-strand	92-95	4	17
α-helix	97	1
β-strand	98	1	17
α-helix	99	1
β-strand	122-124	3	17

Chain P: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	2-28	27
α-helix	29-31	3

Chain R: 15 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	32-52	21
α-helix	54-56	3
β-strand	71	1	18
β-strand	78-80	3	19
β-strand	82	1	18
α-helix	89-92	4
β-strand	101-103	3	19
β-strand	109	1	19
α-helix	128-161	34
α-helix	168-193	26
α-helix	209-245	37
α-helix	253-276	24
α-helix	277-281	5
α-helix	293-327	35
α-helix	337-350	14
α-helix	353-355	3
α-helix	363-366	4
α-helix	371-389	19
α-helix	390-394	5
α-helix	397-409	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-3,Isoform Gnas-2 of Guanine…	A	protein	373	Homo sapiens	P08754 (AlphaFold model), P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Single-chain variable fragment 16	E	protein	297	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	160	Lama glama
Gastric inhibitory polypeptide	P	protein	42	Homo sapiens	P09681
Gastric inhibitory polypeptide receptor	R	protein	463	Homo sapiens	P48546

Sequence of entity 1 (A), FASTA

>7RA3_1 Guanine nucleotide-binding protein G(i) subunit alpha-3,Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNG
FNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFD
FPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCR
VLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDN
QTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTP
EDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRD
IIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>7RA3_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (E), FASTA

>7RA3_3 Single-chain variable fragment 16 (chains E)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFADVQLVESGGGLVQPGGSRKLSC
SASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQ
MTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELKAAAHHHHHHHH

Sequence of entity 4 (G), FASTA

>7RA3_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (N), FASTA

>7RA3_5 Nanobody 35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA

Sequence of entity 6 (P), FASTA

>7RA3_6 Gastric inhibitory polypeptide (chains P)
YAEGTFISDYSIAMDKIHQQDFVNWLLAQKGKKNDWKHNITQ

Sequence of entity 7 (R), FASTA

>7RA3_7 Gastric inhibitory polypeptide receptor (chains R)
DYKDDDDAAALEVLFQGPRAETGSKGQTAGELYQRWERYRRECQETLAAAEPPSGLACNG
SFDMYVCWDYAAPNATARASCPWYLPWHHHVAAGFVLRQCGSDGQWGLWRDHTQCENPEK
NEAFLDQRLILERLQVMYTVGYSLSLATLLLALLILSLFRRLHCTRNYIHINLFTSFMLR
AAAILSRDRLLPRPGPYLGDQALALWNQALAACRTAQIVTQYCVGANYTWLLVEGVYLHS
LLVLVGGSEEGHFRYYLLLGWGAPALFVIPWVIVRYLYENTQCWERNEVKAIWWIIRTPI
LMTILINFLIFIRILGILLSKLRTRQMRCRDYRLRLARSTLTLVPLLGVHEVVFAPVTEE
QARGALRFAKLGFEIFLSSFQGFLVSVLYCFINKEVQSEIRRGWHHCRLRRSLGEEQRQL
PERAFRALPSGSGPGEVPTSRGLSSGTLPGPGNEASRELESYC

Primary citation

Structural determinants of dual incretin receptor agonism by tirzepatide. Sun, B., Willard, F.S., Feng, D. et al. Proc Natl Acad Sci U S A (2022) 119:e2116506119-e2116506119. DOI 10.1073/pnas.2116506119 · PubMed

Other PDB entries of the same protein (UniProt P08754 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2ODE 1.9 Å, Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3
9YCX 2.1 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the…
9YCY 2.2 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the Canonical…
8IKL 2.33 Å, Cryo-EM structure of the CD97-G13 complex
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
9VJ6 2.62 Å, Cryo-EM structure of 5-HT1AR-Gi3 in complex with F-15599
7T11 2.7 Å, CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
9VJF 2.7 Å, Cryo-EM structure of 5-HT1AR-Gi3 in complex with buspirone
2IHB 2.71 Å, Crystal structure of the heterodimeric complex of human RGS10 and activated Gi alpha 3
2V4Z 2.8 Å, The crystal structure of the human G-protein subunit alpha (GNAI3) in complex with an…
7KH0 2.8 Å, Cryo-EM structure of the human arginine vasopressin AVP-vasopressin receptor V2R-Gs…

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