7S8L: Mas-related G-protein coupled receptor member X2

CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14. Determined by electron microscopy at 2.45 Å resolution. Released 17 Nov 2021.

Method: Electron microscopy
Resolution: 2.45 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 8,177
Mol. weight: 139.96 kDa
Released: 17 Nov 2021

Explore 7S8L in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7S8L contains 34 α-helices and 61 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 11 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-40	7	1
α-helix	46-48	3
β-strand	69	1	2
β-strand	72-76	5	1
β-strand	79-84	6	1
β-strand	86	1	2
α-helix	96-98	3
β-strand	105-111	7	1
α-helix	115-117	3
α-helix	118-129	12
α-helix	132-134	3
β-strand	138-144	7	1
α-helix	146-155	10
α-helix	160-162	3
α-helix	165-169	5
α-helix	184-201	18
β-strand	211-212	2	1
β-strand	215	1	1
α-helix	224-242	19

Chain C: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-21	17
α-helix	30-33	4
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
α-helix	129-131	3
β-strand	134-139	6	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	175-181	7	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	208-212	5	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-308	5	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain D: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	12-23	12
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain E: 4 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	10-12	3	11
β-strand	19-25	7	10
α-helix	29-31	3
β-strand	33-39	7	11
β-strand	45-51	7	11
β-strand	58-60	3	11
β-strand	69-73	5	10
β-strand	78-82	5	10
α-helix	88-90	3
β-strand	92-99	8	11
β-strand	111	1	11
β-strand	115-119	5	11
β-strand	129	1	12
β-strand	134-136	3	13
β-strand	143-148	6	12
β-strand	154	1	14
β-strand	160	1	14
β-strand	162-167	6	13
β-strand	174-178	5	13
β-strand	182-183	2	13
α-helix	184	1
β-strand	191-196	6	12
β-strand	199-204	6	12
β-strand	214-219	6	13
α-helix	225	1
β-strand	226-227	2	13
β-strand	231-234	4	13

Chain R: 12 helices, 0 β-strands

Element	Residues	Length
α-helix	31-53	23
α-helix	54-58	5
α-helix	65-94	30
α-helix	104-132	29
α-helix	134-139	6
α-helix	145-166	22
α-helix	178-210	33
α-helix	219-241	23
α-helix	242-246	5
α-helix	247-249	3
α-helix	257-276	20
α-helix	277-281	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Mas-related G-protein coupled receptor member X2	R	protein	331	Homo sapiens	Q96LB1 (AlphaFold model)
Cortistatin 14	A	protein	14	Homo sapiens	O00230 (AlphaFold model)
Gs-mini-Gq chimera	B	protein	246	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	345	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv16	E	protein	257	Mus musculus

Sequence of entity 1 (R), FASTA

>7S8L_1 Mas-related G-protein coupled receptor member X2 (chains R)
GPDPTTPAWGTESTTVNGNDQALLLLCGKETLIPVFLILFIALVGLVGNGFVLWLLGFRM
RRNAFSVYVLSLAGADFLFLCFQIINCLVYLSNFFCSISINFPSFFTTVMTCAYLAGLSM
LSTVSTERCLSVLWPIWYRCRRPRHLSAVVCVLLWALSLLLSILEGKFCGFLFSDGDSGW
CQTFDFITAAWLIFLFMVLCGSSLALLVRILCGSRGLPLTRLYLTILLTVLVFLLCGLPF
GIQWFLILWIWKDSDVLFCHIHPVSVVLSSLNSSANPIIYFFVGSFRKQWRLQQPILKLA
LQRALQDIAEVDHSEGCFRQGTPEMSRSSLV

Sequence of entity 2 (A), FASTA

>7S8L_2 Cortistatin 14 (chains A)
PCKNFFWKTFSSCK

Sequence of entity 3 (B), FASTA

>7S8L_3 Gs-mini-Gq chimera (chains B)
MGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNL
REYNLV

Sequence of entity 4 (C), FASTA

>7S8L_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
GPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (D), FASTA

>7S8L_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (E), FASTA

>7S8L_6 scFv16 (chains E)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAALEVLFQ

Primary citation

Structure, function and pharmacology of human itch GPCRs. Cao, C., Kang, H.J., Singh, I. et al. Nature (2021) 600:170-175. DOI 10.1038/s41586-021-04126-6 · PubMed

Other PDB entries of the same protein (UniProt Q96LB1 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7S8M 2.54 Å, CryoEM structure of Gi-coupled MRGPRX2 with peptide agonist Cortistatin-14
7S8O 2.58 Å, CryoEM structure of Gi-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
7VV5 2.76 Å, Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with C48/80, state1
9WVX 2.8 Å, CryoEM structure of MRGPRX2 with peptide agonist DKD2
7VUY 2.84 Å, Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with PAMP-12. state1
7VUZ 2.89 Å, Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with PAMP-12, state2
7S8N 2.9 Å, CryoEM structure of Gq-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
7VDH 2.9 Å, Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with C48/80, state2
7VV3 2.97 Å, Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with linear cortistatin-14
7VV4 2.97 Å, Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with linear cortistatin-14,…
7VDM 2.98 Å, Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with substance P
7VDL 3.22 Å, Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with circular cortistatin-14

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