7WIC: SS-14-bound human SSTR2-Gi1 complex

Cryo-EM structure of the SS-14-bound human SSTR2-Gi1 complex. Determined by electron microscopy at 2.8 Å resolution. Released 1 Jun 2022.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Homo sapiens, Rattus norvegicus, Bos taurus
Chains: 6
Atoms: 9,048
Mol. weight: 157.88 kDa
Released: 1 Jun 2022

Explore 7WIC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7WIC contains 35 α-helices and 65 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-40	7	3
α-helix	46-52	7
β-strand	185-190	6	3
β-strand	195-200	6	3
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	3
β-strand	233	1	4
β-strand	241	1	4
α-helix	242-254	13
β-strand	263-269	7	3
α-helix	271-278	8
α-helix	283-285	3
α-helix	296-308	13
β-strand	319-323	5	3
α-helix	331-351	21

Chain B: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
α-helix	34-36	3
β-strand	47-51	5	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	88-94	7	6
β-strand	100-105	6	7
β-strand	111-116	6	7
β-strand	121-125	5	7
β-strand	134-139	6	7
β-strand	146-151	6	8
β-strand	156-161	6	8
β-strand	165-170	6	8
β-strand	175-181	7	8
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	207-212	6	9
β-strand	218-222	5	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	259-264	6	10
β-strand	273-278	6	11
β-strand	284-289	6	11
β-strand	294-298	5	11
β-strand	306-308	3	11
β-strand	315-317	3	5
β-strand	320	1	5
β-strand	327-331	5	5
β-strand	335-339	5	5

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-43	14
α-helix	53-55	3

Chain L: 0 helices, 2 β-strands

Element	Residues	Length	Sheet
β-strand	7	1	1
β-strand	10	1	1

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	42-68	27
α-helix	77-95	19
α-helix	98-104	7
α-helix	113-145	33
α-helix	150-153	4
α-helix	156-173	18
α-helix	175-180	6
β-strand	181-182	2	2
β-strand	194-195	2	2
α-helix	203-213	11
α-helix	214-218	5
α-helix	219-242	24
α-helix	247-281	35
α-helix	284-286	3
α-helix	288-309	22
α-helix	310-314	5
α-helix	319-326	8

Chain S: 6 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	12
β-strand	10-12	3	13
β-strand	17-25	9	12
β-strand	33-40	8	13
β-strand	44-51	8	13
α-helix	53-55	3
β-strand	58-60	3	13
β-strand	68-73	6	12
β-strand	78-84	7	12
α-helix	88-90	3
β-strand	92-99	8	13
β-strand	110-111	2	13
β-strand	115-119	5	13
β-strand	140-141	2	14
β-strand	146-148	3	15
β-strand	155-161	7	14
β-strand	166	1	16
β-strand	172	1	16
β-strand	174-179	6	15
β-strand	186-187	2	15
β-strand	189	1	17
α-helix	194	1
β-strand	195	1	17
α-helix	196	1
β-strand	203-208	6	14
β-strand	211-216	6	14
α-helix	221-223	3
β-strand	226-231	6	15
α-helix	237	1
β-strand	243-246	4	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
somatostatin-14	L	protein	14	Homo sapiens	P61278 (AlphaFold model)
Somatostatin receptor type 2	R	protein	369	Homo sapiens	P30874 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	70	Bos taurus	P63212
single Fab chain (svFv16)	S	protein	269	synthetic construct

Sequence of entity 1 (L), FASTA

>7WIC_1 somatostatin-14 (chains L)
AGCKNFFWKTFTSC

Sequence of entity 2 (R), FASTA

>7WIC_2 Somatostatin receptor type 2 (chains R)
MDMADEPLNGSHTWLSIPFDLNGSVVSTNTSNQTEPYYDLTSNAVLTFIYFVVCIIGLCG
NTLVIYVILRYAKMKTITNIYILNLAIADELFMLGLPFLAMQVALVHWPFGKAICRVVMT
VDGINQFTSIFCLTVMSIDRYLAVVHPIKSAKWRRPRTAKMITMAVWGVSLLVILPIMIY
AGLRSNQWGRSSCTINWPGESGAWYTGFIIYTFILGFLVPLTIICLCYLFIIIKVKSSGI
RVGSSKRKKSEKKVTRMVSIVVAVFIFCWLPFYIFNVSSVSMAISPTPALKGMFDFVVVL
TYANSCANPILYAFLSDNFKKSFQNVLCLVKVSGTDDGERSDSKQDKSRLNETTETQRTL
LNGDLQTSI

Sequence of entity 3 (A), FASTA

>7WIC_3 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 4 (B), FASTA

>7WIC_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (G), FASTA

>7WIC_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFCAIL

Sequence of entity 6 (S), FASTA

>7WIC_6 single Fab chain (svFv16) (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQGPAAAHHHHHHHH

Primary citation

Structures of the endogenous peptide- and selective non-peptide agonist-bound SSTR2 signaling complexes. Chen, L.N., Wang, W.W., Dong, Y.J. et al. Cell Res (2022) 32:785-788. DOI 10.1038/s41422-022-00669-z · PubMed

Other PDB entries of the same protein (UniProt P61278 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
8ZBI 2.79 Å, Cryo-EM structure of Somatostatin receptor 3 (SSTR3) with Gi1 complex
7XAT 2.85 Å, Structure of somatostatin receptor 2 bound with SST14.
7XMS 2.9 Å, CryoEM structure of somatostatin receptor 4 (SSTR4) in complex with Gi1 and its…
8ZBJ 2.94 Å, Cryo-EM structure of Somatostatin receptor 5 (SSTR5) with Gi1 complex
7XMR 3.1 Å, CryoEM structure of the somatostatin receptor 2 (SSTR2) in complex with Gi1 and its…
7Y27 3.48 Å, Cryo-EM structure of the SST-14-bound SSTR2-miniGq-scFv16 complex
7WJ5 3.72 Å, Cryo-EM structure of human somatostatin receptor 2 complex with its agonist somatostatin…
2MI1 Somatostatin-14 solution structure in 5% D-mannitol

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