8ZBJ: Somatostatin receptor 5 (SSTR5) with Gi1 complex

Cryo-EM structure of Somatostatin receptor 5 (SSTR5) with Gi1 complex. Determined by electron microscopy at 2.94 Å resolution. Released 30 Apr 2025.

Method: Electron microscopy
Resolution: 2.94 Å
Organism: Homo sapiens
Chains: 6
Atoms: 8,085
Mol. weight: 170.27 kDa
Released: 30 Apr 2025

Explore 8ZBJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8ZBJ contains 26 α-helices and 65 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-39	6	18
β-strand	185-190	6	18
β-strand	195-200	6	18
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-225	6	18
α-helix	243-254	12
α-helix	257-259	3
β-strand	263-268	6	18
α-helix	300-310	11
β-strand	319-323	5	18
α-helix	330-351	22

Chain C: 2 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	6-25	20
α-helix	30-34	5
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-179	5	5
β-strand	187	1	6
β-strand	191-192	2	7
β-strand	198-202	5	7
β-strand	203	1	6
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	260-264	5	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-295	2	10
β-strand	296-298	3	9
β-strand	307-308	2	10
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain E: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	11-23	13
α-helix	30-43	14

Chain F: 3 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	41-46	6	11
β-strand	48-50	3	12
β-strand	56-63	8	11
α-helix	67-69	3
β-strand	71-77	7	13
β-strand	83-89	7	13
β-strand	96-98	3	13
β-strand	106-111	6	11
β-strand	116-121	6	11
α-helix	126-128	3
β-strand	131-137	7	13
β-strand	149	1	13
β-strand	151	1	11
β-strand	153-154	2	13
β-strand	155-157	3	12
β-strand	178-179	2	14
β-strand	195-197	3	15
β-strand	198-199	2	14
β-strand	204	1	16
β-strand	210	1	16
β-strand	212-217	6	17
β-strand	224-228	5	17
β-strand	232-233	2	17
α-helix	234	1
β-strand	242-244	3	15
β-strand	250-253	4	15
β-strand	264-269	6	17
β-strand	277	1	17
β-strand	281-282	2	17

Chain R: 12 helices, 0 β-strands

Element	Residues	Length
α-helix	42-66	25
α-helix	75-91	17
α-helix	94-102	9
α-helix	109-142	34
α-helix	153-169	17
α-helix	172-177	6
α-helix	194-204	11
α-helix	205-211	7
α-helix	212-228	17
α-helix	241-273	33
α-helix	280-305	26
α-helix	309-315	7

Chain S: 0 helices, 2 β-strands

Element	Residues	Length	Sheet
β-strand	6	1	1
β-strand	11	1	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Somatostatin-14	S	protein	14	Homo sapiens	P61278 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	345	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	E	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv16	F	protein	296	Homo sapiens
Soluble cytochrome b562,Somatostatin receptor type 5	R	protein	470	Homo sapiens	P35346 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096

Sequence of entity 1 (S), FASTA

>8ZBJ_1 Somatostatin-14 (chains S)
AGCKNFFWKTFTSC

Sequence of entity 2 (C), FASTA

>8ZBJ_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
GPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (E), FASTA

>8ZBJ_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (F), FASTA

>8ZBJ_4 scFv16 (chains F)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFADVQLVESGGGLVQPGGSRKLSC
SASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQ
MTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELKAAAENLYFQG

Sequence of entity 5 (R), FASTA

>8ZBJ_5 Soluble cytochrome b562,Somatostatin receptor type 5 (chains R)
ADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKD
FRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLMEPLFPASTPSWNA
SSPGAASGGGDNRTLVGPAPSAGARAVLVPVLYLLVCAAGLGGNTLVIYVVLRFAKMKTV
TNIYILNLAVADVLYMLGLPFLATQNAASFWPFGPVLCRLVMTLDGVNQFTSVFCLTVMS
VDRYLAVVHPLSSARWRRPRVAKLASAAAWVLSLCMSLPLLVFADVQEGGTCNASWPEPV
GLWGAVFIIYTAVLGFFAPLLVICLCYLLIVVKVRAAGVRVGCVRRRSERKVTRMVLVVV
LVFAGCWLPFFTVNIVNLAVALPQEPASAGLYFFVVILSYANSCANPVLYGFLSDNFRQS
FQKVLCLRKGSGAKDADATEPRPDRIRQQQEATPPAHRAAANGLMQTSKL

Sequence of entity 6 (A), FASTA

>8ZBJ_6 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Primary citation

The structure of SSTR3 and SSTR5 provide insights into the specificity among somatostatin receptos. Heo, Y., Kim, Y., Cho, M. et al. To be published.

Other PDB entries of the same protein (UniProt P61278 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
8ZBI 2.79 Å, Cryo-EM structure of Somatostatin receptor 3 (SSTR3) with Gi1 complex
7WIC 2.8 Å, Cryo-EM structure of the SS-14-bound human SSTR2-Gi1 complex
7XAT 2.85 Å, Structure of somatostatin receptor 2 bound with SST14.
7XMS 2.9 Å, CryoEM structure of somatostatin receptor 4 (SSTR4) in complex with Gi1 and its…
7XMR 3.1 Å, CryoEM structure of the somatostatin receptor 2 (SSTR2) in complex with Gi1 and its…
7Y27 3.48 Å, Cryo-EM structure of the SST-14-bound SSTR2-miniGq-scFv16 complex
7WJ5 3.72 Å, Cryo-EM structure of human somatostatin receptor 2 complex with its agonist somatostatin…
2MI1 Somatostatin-14 solution structure in 5% D-mannitol

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