7XAV: Somatostatin receptor 2

Structure of somatostatin receptor 2 bound with lanreotide. Determined by electron microscopy at 2.87 Å resolution. Released 31 Aug 2022.

Method: Electron microscopy
Resolution: 2.87 Å
Organisms: Homo sapiens, synthetic construct, Bos taurus
Chains: 6
Atoms: 8,410
Mol. weight: 184.45 kDa
Released: 31 Aug 2022

Explore 7XAV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XAV contains 41 α-helices and 64 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	44-60	17
α-helix	62-65	4
α-helix	78-91	14
α-helix	96-100	5
α-helix	101-103	3
α-helix	114-124	11
α-helix	136-144	9
α-helix	165-169	5
α-helix	175-179	5
β-strand	183-184	2	1
β-strand	192-193	2	1
α-helix	208-213	6
α-helix	220-235	16
α-helix	247-250	4
α-helix	251-254	4
α-helix	260-280	21
α-helix	283-285	3
α-helix	290-293	4
α-helix	295-298	4
α-helix	304-311	8
α-helix	317-325	9

Chain B: 12 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	9-25	17
β-strand	33-40	8	2
α-helix	46-52	7
β-strand	185-188	4	2
β-strand	195-200	6	2
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-225	6	2
α-helix	227-229	3
α-helix	233-234	2
α-helix	244-254	11
α-helix	263	1
β-strand	264-268	5	2
α-helix	271-278	8
α-helix	299-301	3
α-helix	303-308	6
β-strand	321-323	3	2
α-helix	333-350	18

Chain C: 3 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	46-52	7	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-151	6	6
β-strand	157-161	5	6
β-strand	165-168	4	6
β-strand	170	1	7
β-strand	175	1	7
β-strand	180-181	2	6
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	208-212	5	8
β-strand	220-222	3	8
β-strand	229-234	6	9
β-strand	241-245	5	9
β-strand	250-252	3	9
β-strand	262-264	3	9
β-strand	275-277	3	10
β-strand	284-288	5	10
β-strand	294-298	5	10
β-strand	303-308	6	10
β-strand	319-320	2	3
β-strand	327-330	4	3
β-strand	335-340	6	3

Chain D: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	7-21	15
α-helix	27-29	3
α-helix	30-33	4
α-helix	35-41	7
α-helix	53-55	3

Chain E: 2 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	11
β-strand	12	1	12
β-strand	17-25	9	11
α-helix	29-31	3
β-strand	33-39	7	13
β-strand	45-51	7	13
β-strand	58-60	3	13
β-strand	68-73	6	11
β-strand	78-84	7	11
β-strand	92-99	8	13
β-strand	111	1	13
β-strand	115-117	3	13
β-strand	119	1	12
β-strand	141	1	14
β-strand	146-148	3	15
α-helix	154	1
β-strand	155-160	6	14
β-strand	166	1	16
β-strand	172	1	16
β-strand	174-179	6	15
β-strand	185-187	3	15
β-strand	189-190	2	17
β-strand	194-195	2	17
β-strand	203-208	6	14
β-strand	211-216	6	14
β-strand	225-231	7	15
β-strand	239	1	15
β-strand	243-246	4	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Somatostatin receptor type 2,LargeBit	A	protein	563	Homo sapiens, synthetic construct	P30874 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	B	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	354	Bos taurus	P62871 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	71	Bos taurus	P63212 (AlphaFold model)
ScFv16	E	protein	304	Rattus
Lanreotide	F	protein	9	synthetic construct

Sequence of entity 1 (A), FASTA

>7XAV_1 Somatostatin receptor type 2,LargeBit (chains A)
MKTIIALSYIFCLVFADYKDDDDKGSGSHHHHHHHHHHLEVLFQGPMDMADEPLNGSHTW
LSIPFDLNGSVVSTNTSNQTEPYYDLTSNAVLTFIYFVVCIIGLCGNTLVIYVILRYAKM
KTITNIYILNLAIADELFMLGLPFLAMQVALVHWPFGKAICRVVMTVDGINQFTSIFCLT
VMSIDRYLAVVHPIKSAKWRRPRTAKMITMAVWGVSLLVILPIMIYAGLRSNQWGRSSCT
INWPGESGAWYTGFIIYTFILGFLVPLTIICLCYLFIIIKVKSSGIRVGSSKRKKSEKKV
TRMVSIVVAVFIFCWLPFYIFNVSSVSMAISPTPALKGMFDFVVVLTYANSCANPILYAF
LSDNFKKSFQNVLCLVKVSGTDDGERSDSKQDKSRLNETTETQRTVFTLEDFVGDWEQTA
AYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIE
EVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNG
NKIIDERLITPDGSMLFRVTINS

Sequence of entity 2 (B), FASTA

>7XAV_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains B)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (C), FASTA

>7XAV_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSS

Sequence of entity 4 (D), FASTA

>7XAV_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (E), FASTA

>7XAV_5 ScFv16 (chains E)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFDVQLVESGGGLVQPGGSRKLSCS
ASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQM
TSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSSDIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELVDENLYFQGASHHHH
HHHH

Sequence of entity 6 (F), FASTA

>7XAV_6 Lanreotide (chains F)
XCYWKVCTX

Primary citation

Structural insights into the activation of somatostatin receptor 2 by cyclic SST analogues. Bo, Q., Yang, F., Li, Y. et al. Cell Discov (2022) 8:47-47. DOI 10.1038/s41421-022-00405-2 · PubMed

Other PDB entries of the same protein (UniProt P30874 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
7XN9 2.6 Å, Crystal structure of SSTR2 and L-054,522 complex
7XNA 2.65 Å, Crystal structure of somatostatin receptor 2 (SSTR2) with peptide antagonist CYN 154806
7T11 2.7 Å, CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
7WIG 2.7 Å, Cryo-EM structure of the L-054,264-bound human SSTR2-Gi1 complex
7WIC 2.8 Å, Cryo-EM structure of the SS-14-bound human SSTR2-Gi1 complex
7XAT 2.85 Å, Structure of somatostatin receptor 2 bound with SST14.
7XAU 2.97 Å, Structure of somatostatin receptor 2 bound with octreotide.
7UL5 3.1 Å, CryoEM Structure of Inactive SSTR2 bound to Nb6
7XMR 3.1 Å, CryoEM structure of the somatostatin receptor 2 (SSTR2) in complex with Gi1 and its…
7YAC 3.24 Å, Paltusotine-bound SSTR2-Gi complex
7Y24 3.25 Å, Cryo-EM structure of the octreotide-bound SSTR2-miniGo-scFv16 complex

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