7XP5: Class T GPCR in ligand-free state

Cryo-EM structure of a class T GPCR in ligand-free state. Determined by electron microscopy at 3.08 Å resolution. Released 12 Oct 2022.

Method: Electron microscopy
Resolution: 3.08 Å
Organisms: Acetivibrio thermocellus, Homo sapiens
Chains: 5
Atoms: 8,381
Mol. weight: 184.56 kDa
Released: 12 Oct 2022

Explore 7XP5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XP5 contains 31 α-helices and 45 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-35	30
β-strand	36-43	8	1
α-helix	49-59	11
β-strand	72-79	8	1
β-strand	82-89	8	1
α-helix	99-103	5
β-strand	108-114	7	1
α-helix	121-133	13
β-strand	141-147	7	1
α-helix	149-158	10
α-helix	163-166	4
α-helix	168-171	4
α-helix	187-204	18
β-strand	214-217	4	1
α-helix	225-246	22

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-44	15
α-helix	45-47	3
α-helix	53-55	3

Chain N: 1 helix, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	11	1	10
β-strand	18-25	8	9
β-strand	34-39	6	11
β-strand	45-51	7	11
β-strand	58-60	3	11
α-helix	62-64	3
β-strand	68-73	6	9
β-strand	78-83	6	9
β-strand	93-98	6	11
β-strand	122-123	2	11
β-strand	125	1	10

Chain R: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	4-38	35
α-helix	43-66	24
α-helix	67-71	5
α-helix	78-110	33
α-helix	116-123	8
α-helix	125-154	30
α-helix	156-160	5
α-helix	165-173	9
α-helix	177-180	4
α-helix	181-185	5
α-helix	186-212	27
α-helix	219-254	36
α-helix	258-282	25
α-helix	284-300	17

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Endoglucanase H,Taste receptor type 2 member 46,Bitter taste receptor T2R46	R	protein	802	Acetivibrio thermocellus, Homo sapiens	P16218 (AlphaFold model), P59540 (AlphaFold model)
Guanine nucleotide-binding protein G(t) subunit alpha-3	A	protein	264	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	366	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
Nanobody 35	N	protein	139	Homo sapiens

Sequence of entity 1 (R), FASTA

>7XP5_1 Endoglucanase H,Taste receptor type 2 member 46,Bitter taste receptor T2R46 (chains R)
MKTIIALSYIFCLVFADYKDDDDAHHHHHHHHHHENLYFQSGRAMASNYNSGLKIGAWVG
TQPSESAIKSFQELQGRKLDIVHQFINWSTDFSWVRPYADAVYNNGSILMITWEPWEYNT
VDIKNGKADAYITRMAQDMKAYGKEIWLRPLHAANGDWYPWAIGYSSRVNTNETYIAAFR
HIVDIFRANGATNVKWVFNVNCDNVGNGTSYLGHYPGDNYVDYTSIDGYNWGTTQSWGSQ
WQSFDQVFSRAYQALASINKPIIIAEFASAEIGGNKARWITEAYNSIRTSYNKVIAAVWF
HENKETDWRINSSPEALAAYREAIGAITFLPIIFSILIVVTFVIGNFANGFIALVNSIEW
FKRQKISFADQILTALAVSRVGLLWVLVLNWYATELNPAFNSIEVRITAYNVWAVINHFS
NWLATSLSIFYLLKIANFSNLIFLHLKRRVKSVVLVILLGPLLFLVCHLFVINMNQIIWT
KEYEGNMTWKIKLRSAMYLSNTTVTILANLVPFTLTLISFLLLICSLCKHLKKMQLHGKG
SQDPSMKVHIKALQTVTSFLLLCAIYFLSIIMSVWSFESLENKPVFMFCEAIAFSYPSTH
PFILIWGNKKLKQTFLSVLWHVRYWVKGEKPSSSGSGSSGSGSSVFTLEDFVGDWEQTAA
YNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEE
VFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGN
KIIDERLITPDGSMLFRVTINS

Sequence of entity 2 (A), FASTA

>7XP5_2 Guanine nucleotide-binding protein G(t) subunit alpha-3 (chains A)
MDYKDDDDKENLYFQSNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAD
NSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFND
VTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIED
YFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTQ
NVKFVFDAVTDIIIKENLKDCGLF

Sequence of entity 3 (B), FASTA

>7XP5_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWR
LFKKIS

Sequence of entity 4 (G), FASTA

>7XP5_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (N), FASTA

>7XP5_5 Nanobody 35 (chains N)
MQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASIS
YTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRG
QGTQVTVSSHHHHHHEPEA

Primary citation

Structural basis for strychnine activation of human bitter taste receptor TAS2R46. Xu, W., Wu, L., Liu, S. et al. Science (2022) 377:1298-1304. DOI 10.1126/science.abo1633 · PubMed

Other PDB entries of the same protein (UniProt P16218 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2V3G 1.2 Å, Structure of a family 26 lichenase in complex with noeuromycin
2CIP 1.4 Å, Structure of the Michaelis complex of a family 26 lichenase
2CIT 1.4 Å, Structure of the covalent intermediate of a family 26 lichenase
6R3M 1.45 Å, Family 11 Carbohydrate-Binding Module from Clostridium thermocellum in complex with…
2BV9 1.5 Å, How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides.…
2VI0 1.51 Å, Lichenase CtLic26 in complex with a thio-oligosaccharide
2BVD 1.6 Å, How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides.…
1V0A 1.98 Å, Family 11 Carbohydrate-Binding Module of cellulosomal cellulase Lic26A-Cel5E of…
4U3A 2.42 Å, Crystal structure of CtCel5E
4U5I 2.5 Å, Complex structure of mutant CtCel5E (E314A) with xylobiose
5BYW 2.6 Å, Crystal structure of engineered trifunctional CtCEL5E
6R31 2.6 Å, Family 11 Carbohydrate-Binding Module from Clostridium thermocellum in complex with…

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