7YAE: Octreotide-bound SSTR2-Gi complex

Octreotide-bound SSTR2-Gi complex. Determined by electron microscopy at 3.37 Å resolution. Released 19 Apr 2023.

Method: Electron microscopy
Resolution: 3.37 Å
Organisms: Homo sapiens, Mus musculus, synthetic construct
Chains: 6
Atoms: 9,031
Mol. weight: 158.7 kDa
Ligands: CLR
Released: 19 Apr 2023

Explore 7YAE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7YAE contains 30 α-helices and 65 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-27	21
β-strand	34-39	6	1
β-strand	185-190	6	1
β-strand	195-200	6	1
α-helix	208-210	3
α-helix	212-214	3
β-strand	221-224	4	1
α-helix	242-254	13
β-strand	264-270	7	1
α-helix	271-280	10
α-helix	283-286	4
α-helix	296-308	13
β-strand	319-324	6	1
α-helix	330-351	22

Chain B: 3 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	2-24	23
α-helix	30-34	5
α-helix	38-41	4
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	103-105	3	4
β-strand	111-114	4	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	148-151	4	5
β-strand	156-160	5	5
β-strand	166-170	5	5
β-strand	178-180	3	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	220-222	3	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	249-254	6	7
β-strand	262-265	4	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315	1	9
β-strand	320	1	2
β-strand	327-329	3	2
β-strand	331	1	9
β-strand	336-339	4	2

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-21	14
α-helix	30-44	15
α-helix	45-47	3

Chain E: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	43-71	29
α-helix	77-92	16
α-helix	96-103	8
α-helix	112-145	34
α-helix	158-173	16
α-helix	177-180	4
α-helix	207-213	7
α-helix	214-218	5
α-helix	219-239	21
α-helix	247-250	4
α-helix	253-281	29
α-helix	288-303	16
α-helix	307-312	6
α-helix	318-321	4

Chain S: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	11-12	2	11
β-strand	15	1	12
β-strand	19	1	13
β-strand	21-25	5	10
α-helix	29-31	3
β-strand	34-38	5	14
β-strand	46-51	6	14
β-strand	58	1	14
β-strand	68-73	6	13
β-strand	78-83	6	13
β-strand	85	1	12
β-strand	92-94	3	15
β-strand	98	1	16
β-strand	110	1	16
β-strand	115-117	3	15
β-strand	118-119	2	11
β-strand	146-148	3	17
β-strand	155-160	6	18
β-strand	174-180	7	19
β-strand	183-190	8	19
β-strand	194-195	2	19
β-strand	203	1	18
β-strand	206-207	2	18
β-strand	211-216	6	18
α-helix	221-223	3
β-strand	226-231	6	19
β-strand	238	1	14
β-strand	239	1	19
β-strand	243	1	19
β-strand	244-246	3	17

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv16	S	protein	259	Mus musculus
Somatostatin receptor type 2	E	protein	377	Homo sapiens	P30874 (AlphaFold model)
Dpn-cys-phe-dtr-lys-thr-cys-tho	D	protein	8	synthetic construct

Sequence of entity 1 (A), FASTA

>7YAE_1 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 2 (B), FASTA

>7YAE_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>7YAE_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (S), FASTA

>7YAE_4 scFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAHHHHHHHH

Sequence of entity 5 (E), FASTA

>7YAE_5 Somatostatin receptor type 2 (chains E)
DYKDDDDAMDMADEPLNGSHTWLSIPFDLNGSVVSTNTSNQTEPYYDLTSNAVLTFIYFV
VCIIGLCGNTLVIYVILRYAKMKTITNIYILNLAIADELFMLGLPFLAMQVALVHWPFGK
AICRVVMTVDGINQFTSIFCLTVMSIDRYLAVVHPIKSAKWRRPRTAKMITMAVWGVSLL
VILPIMIYAGLRSNQWGRSSCTINWPGESGAWYTGFIIYTFILGFLVPLTIICLCYLFII
IKVKSSGIRVGSSKRKKSEKKVTRMVSIVVAVFIFCWLPFYIFNVSSVSMAISPTPALKG
MFDFVVVLTYANSCANPILYAFLSDNFKKSFQNVLCLVKVSGTDDGERSDSKQDKSRLNE
TTETQRTLLNGDLQTSI

Sequence of entity 6 (D), FASTA

>7YAE_6 DPN-CYS-PHE-DTR-LYS-THR-CYS-THO (chains D)
FCFWKTCX

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	2

Primary citation

Prospect of acromegaly therapy: molecular mechanism of clinical drugs octreotide and paltusotine. Zhao, J., Fu, H., Yu, J. et al. Nat Commun (2023) 14:962-962. DOI 10.1038/s41467-023-36673-z · PubMed

Other PDB entries of the same protein (UniProt P63096 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
3UMR 2.04 Å, Crystal structure of the G202D mutant of human G-alpha-i1
9P7Z 2.1 Å, NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
2OM2 2.2 Å, Crystal Structure Of Human G[alpha]i1 Bound To The Goloco Motif Of Rgs14
8YN9 2.3 Å, Cryo-EM structure of histamine H4 receptor in complex with histamine and Gi
9HYI 2.3 Å, DP81-bound serotonin 5-HT1A receptor - Gi Protein Complex
9O36 2.3 Å, CryoEM structure of mu-opioid receptor - Gi protein complex bound to fluornitrazene (FNZ)
9P80 2.3 Å, NTSR1-Gi-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
8XXV 2.33 Å, Cryo-EM Structure of the Prostaglandin D2 Receptor 2-indomethacin Coupled to G Protein
3UMS 2.34 Å, Crystal structure of the G202A mutant of human G-alpha-i1
3ONW 2.38 Å, Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.
20ZG 2.4 Å, Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the…

Browse structure collections

About this viewer

MolViewer shows 7YAE directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.