Architecture of the ESCPE-1 membrane coat. Determined by electron microscopy at 10.0 Å resolution. Released 21 Jun 2023.
Explore 8AFZ in 3D Show helices and sheets RCSB PDB PDBe
8AFZ contains 28 α-helices and 8 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 145-155 | 11 | 1 |
| α-helix | 158-160 | 3 | |
| β-strand | 162-171 | 10 | 1 |
| β-strand | 180-186 | 7 | 1 |
| α-helix | 187-201 | 15 | |
| α-helix | 202-204 | 3 | |
| α-helix | 209-213 | 5 | |
| α-helix | 216-223 | 8 | |
| α-helix | 229-251 | 23 | |
| α-helix | 253-256 | 4 | |
| α-helix | 259-263 | 5 | |
| α-helix | 276-299 | 24 | |
| α-helix | 307-357 | 51 | |
| α-helix | 361-388 | 28 | |
| α-helix | 389-393 | 5 | |
| α-helix | 394-439 | 46 | |
| α-helix | 443-519 | 77 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 31-41 | 11 | 2 |
| β-strand | 44-53 | 10 | 2 |
| β-strand | 62-68 | 7 | 2 |
| α-helix | 69-81 | 13 | |
| α-helix | 83-85 | 3 | |
| α-helix | 90-97 | 8 | |
| α-helix | 100-111 | 12 | |
| α-helix | 113-115 | 3 | |
| α-helix | 118-151 | 34 | |
| α-helix | 156-158 | 3 | |
| α-helix | 160-166 | 7 | |
| α-helix | 180-199 | 20 | |
| α-helix | 211-256 | 46 | |
| α-helix | 260-287 | 28 | |
| α-helix | 288-292 | 5 | |
| α-helix | 293-332 | 40 | |
| α-helix | 336-402 | 67 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 173-179 | 7 | 2 |
| β-strand | 192-198 | 7 | 2 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Sorting nexin-1 | A | protein | 522 | Homo sapiens | Q13596 (AlphaFold model) |
| Sorting nexin-5 | B | protein | 404 | Homo sapiens | Q9Y5X3 (AlphaFold model) |
| Cation-independent mannose-6-phosphate receptor | C | protein | 31 | Homo sapiens | P11717 (AlphaFold model) |
>8AFZ_1 Sorting nexin-1 (chains A) MASGGGGCSASERLPPPFPGLEPESEGAAGGSEPEAGDSDTEGEDIFTGAAVVSKHQSPK ITTSLLPINNGSKENGIHEEQDQEPQDLFADATVELSLDSTQNNQKKVLAKTLISLPPQE ATNSSKPQPTYEELEEEEQEDQFDLTVGITDPEKIGDGMNAYVAYKVTTQTSLPLFRSKQ FAVKRRFSDFLGLYEKLSEKHSQNGFIVPPPPEKSLIGMTKVKVGKEDSSSAEFLEKRRA ALERYLQRIVNHPTMLQDPDVREFLEKEELPRAVGTQTLSGAGLLKMFNKATDAVSKMTI KMNESDIWFEEKLQEVECEEQRLRKLHAVVETLVNHRKELALNTAQFAKSLAMLGSSEDN TALSRALSQLAEVEEKIEQLHQEQANNDFFLLAELLSDYIRLLAIVRAAFDQRMKTWQRW QDAQATLQKKREAEARLLWANKPDKLQQAKDEILEWESRVTQYERDFERISTVVRKEVIR FEKEKSKDFKNHVIKYLETLLYSQQQLAKYWEAFLPEAKAIS
>8AFZ_2 Sorting nexin-5 (chains B) MAAVPELLQQQEEDRSKLRSVSVDLNVDPSLQIDIPDALSERDKVKFTVHTKTTLPTFQS PEFSVTRQHEDFVWLHDTLIETTDYAGLIIPPAPTKPDFDGPREKMQKLGEGEGSMTKEE FAKMKQELEAEYLAVFKKTVSSHEVFLQRLSSHPVLSKDRNFHVFLEYDQDLSVRRKNTK EMFGGFFKSVVKSADEVLFTGVKEVDDFFEQEKNFLINYYNRIKDSCVKADKMTRSHKNV ADDYIHTAACLHSLALEEPTVIKKYLLKVAELFEKLRKVEGRVSSDEDLKLTELLRYYML NIEAAKDLLYRRTKALIDYENSNKALDKARLKSKDVKLAEAHQQECCQKFEQLSESAKEE LINFKRKRVAAFRKNLIEMSELEIKHARNNVSLLQSCIDLFKNN
>8AFZ_3 Cation-independent mannose-6-phosphate receptor (chains C) SNVSYKYSKVNKEEETDENETEWLMEEIQLP
Architecture of the ESCPE-1 membrane coat. Lopez-Robles, C., Scaramuzza, S., Astorga-Simon, E.N. et al. Nat Struct Mol Biol (2023) 30:958-969. DOI 10.1038/s41594-023-01014-7 · PubMed
Other PDB entries of the same protein (UniProt Q13596 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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