8B9A: DNA replication licensing factor MCM2

Name: PDB 8B9A: S. cerevisiae replisome + Ctf4, bound by pol alpha primase. Complex engaged with a fork DNA substrate containing a 60 nucleotide lagging strand.
Published: 2023-08-09
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: replication

S. cerevisiae replisome + Ctf4, bound by pol alpha primase. Complex engaged with a fork DNA substrate containing a 60 nucleotide lagging strand. Determined by electron microscopy at 3.5 Å resolution. Released 9 Aug 2023.

Method: Electron microscopy
Resolution: 3.5 Å
Organism: Saccharomyces cerevisiae
Chains: 23
Atoms: 69,712
Mol. weight: 1843.99 kDa
Ligands: ZN, MG, ANP
Released: 9 Aug 2023

Explore 8B9A in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8B9A contains 456 α-helices and 363 β-strands across 21 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 29 helices, 24 β-strands

Element	Residues	Length	Sheet
α-helix	184-187	4
α-helix	195-198	4
α-helix	201-215	15
β-strand	220	1	1
β-strand	223	1	1
α-helix	226-236	11
β-strand	241-245	5	2
α-helix	246-251	6
α-helix	254-262	9
α-helix	264-282	19
α-helix	286-288	3
β-strand	294-298	5	2
β-strand	304	1	3
α-helix	311-313	3
β-strand	317-327	11	3
α-helix	328-330	3
β-strand	331-334	4	4
β-strand	382-385	4	4
β-strand	386-391	6	3
α-helix	398	1
α-helix	402-404	3
β-strand	405-410	6	3
β-strand	423-433	11	3
α-helix	436-442	7
β-strand	446	1	5
β-strand	448-457	10	3
α-helix	478-489	12
α-helix	493-500	8
α-helix	509-520	12
β-strand	525-527	3	6
β-strand	531-533	3	6
β-strand	539-543	5	7
α-helix	549-559	11
β-strand	563-565	3	7
α-helix	596-599	4
β-strand	603-606	4	7
α-helix	615-625	11
β-strand	628-633	6	8
β-strand	636-641	6	8
β-strand	645-650	6	7
α-helix	663-666	4
α-helix	671-676	6
β-strand	679-683	5	7
α-helix	689-705	17
α-helix	746-755	10
α-helix	760-773	14
β-strand	777	1	9
α-helix	783-799	17
α-helix	802-805	4
α-helix	807-823	17
β-strand	828	1	9
α-helix	831-847	17
α-helix	851-865	15

Chain 3: 32 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	19-34	16
α-helix	37-52	16
α-helix	91-93	3
β-strand	95-99	5	10
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-135	18
α-helix	144-147	4
β-strand	154-158	5	10
α-helix	162-164	3
β-strand	165	1	11
α-helix	173-175	3
β-strand	179-189	11	11
α-helix	190-192	3
β-strand	193-203	11	11
β-strand	209-213	5	11
α-helix	230-232	3
β-strand	234	1	12
β-strand	240	1	12
β-strand	241-243	3	11
β-strand	249-259	11	11
α-helix	262-264	3
α-helix	269-271	3
β-strand	273-279	7	11
α-helix	280-282	3
β-strand	291-301	11	11
β-strand	317	1	13
β-strand	318-328	11	11
α-helix	344-353	10
α-helix	359-365	7
α-helix	375-386	12
β-strand	391-392	2	14
β-strand	398-399	2	14
β-strand	405-409	5	15
α-helix	415-422	8
β-strand	429-433	5	15
β-strand	437	1	16
α-helix	439-443	5
β-strand	444-447	4	17
β-strand	456-459	4	17
α-helix	461-464	4
β-strand	469-473	5	15
α-helix	475-477	3
α-helix	480-483	4
α-helix	484-486	3
β-strand	494-499	6	18
β-strand	502-507	6	18
β-strand	511-516	6	15
α-helix	529-532	4
α-helix	537-540	4
β-strand	545-549	5	15
α-helix	555-569	15
α-helix	653-666	14
α-helix	669	1
β-strand	670	1	19
α-helix	671	1
α-helix	673-688	16
α-helix	699-715	17
β-strand	720	1	19
α-helix	723-738	16

Chain 4: 38 helices, 37 β-strands

Element	Residues	Length	Sheet
β-strand	177-180	4	20
β-strand	186	1	20
α-helix	187-200	14
β-strand	202	1	21
α-helix	204-209	6
α-helix	213-215	3
α-helix	218-221	4
β-strand	224	1	21
α-helix	225-236	12
β-strand	240-244	5	22
α-helix	247-250	4
α-helix	252-254	3
α-helix	255-263	9
α-helix	265-286	22
α-helix	293-298	6
β-strand	302-306	5	22
α-helix	314-316	3
α-helix	319-321	3
β-strand	325-335	11	23
α-helix	336-338	3
β-strand	339-349	11	23
β-strand	355-359	5	23
β-strand	361	1	24
β-strand	364	1	24
β-strand	370	1	25
α-helix	374-376	3
β-strand	379	1	25
β-strand	382-384	3	23
α-helix	386-388	3
β-strand	390-400	11	23
α-helix	403-405	3
α-helix	406-407	2
β-strand	414-420	7	23
α-helix	421-423	3
β-strand	432-442	11	23
β-strand	445	1	26
α-helix	452	1
β-strand	453-454	2	26
β-strand	456	1	27
β-strand	458-468	11	23
α-helix	503-513	11
α-helix	518-524	7
α-helix	534-545	12
β-strand	550-551	2	28
β-strand	557-558	2	28
β-strand	564-568	5	29
α-helix	574-584	11
β-strand	588-592	5	29
β-strand	596	1	30
α-helix	598-601	4
β-strand	603-605	3	31
β-strand	616-618	3	31
α-helix	620-623	4
β-strand	628-632	5	29
α-helix	634-636	3
α-helix	639-642	4
α-helix	645-651	7
β-strand	653-658	6	32
β-strand	661-666	6	32
β-strand	670-675	6	29
α-helix	688-692	5
α-helix	696-699	4
β-strand	704-708	5	29
α-helix	714-726	13
α-helix	727-729	3
α-helix	744-757	14
β-strand	761-762	2	33
α-helix	764-778	15
α-helix	795-811	17
β-strand	816-817	2	33
α-helix	819-832	14
α-helix	835-837	3
β-strand	838	1	34
β-strand	845	1	34
α-helix	847-849	3

Chain 5: 27 helices, 25 β-strands

Element	Residues	Length	Sheet
α-helix	22-37	16
α-helix	45-55	11
β-strand	60-64	5	35
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-100	18
α-helix	134-135	2
β-strand	136-140	5	35
α-helix	143-147	5
α-helix	148-150	3
α-helix	153-155	3
β-strand	158-169	12	13
α-helix	170-172	3
β-strand	173-176	4	13
β-strand	178-183	6	36
β-strand	189-193	5	36
β-strand	241-243	3	36
α-helix	245-247	3
β-strand	250-259	10	13
α-helix	262-264	3
α-helix	266	1
α-helix	270-272	3
β-strand	273-279	7	13
α-helix	280-282	3
β-strand	291-301	11	13
β-strand	323	1	4
β-strand	325-334	10	13
α-helix	351-361	11
α-helix	366-372	7
α-helix	382-393	12
β-strand	398-399	2	37
β-strand	405-406	2	37
β-strand	412-414	3	38
α-helix	422-432	11
β-strand	436-439	4	38
β-strand	451-454	4	39
β-strand	463-466	4	39
β-strand	476-479	4	38
α-helix	482-484	3
α-helix	487-498	12
β-strand	501-506	6	16
β-strand	509-514	6	16
β-strand	518-522	5	38
α-helix	545-549	5
β-strand	552-554	3	38
α-helix	562-576	15
α-helix	596-609	14
β-strand	613-614	2	40
α-helix	616-635	20
α-helix	650-666	17
β-strand	671-672	2	40
α-helix	674-683	10

Chain 6: 36 helices, 33 β-strands

Element	Residues	Length	Sheet
β-strand	94-96	3	41
α-helix	101-103	3
α-helix	106-121	16
α-helix	124	1
β-strand	125	1	42
α-helix	126	1
β-strand	132	1	42
α-helix	135-146	12
β-strand	150-154	5	43
α-helix	155-159	5
α-helix	161-164	4
α-helix	165-172	8
α-helix	174-192	19
α-helix	194-196	3
β-strand	198	1	44
α-helix	259	1
β-strand	260	1	44
α-helix	261	1
β-strand	264-268	5	43
β-strand	274	1	5
α-helix	276-278	3
α-helix	281-283	3
β-strand	287-297	11	5
α-helix	298-300	3
β-strand	301-310	10	5
β-strand	317-322	6	5
β-strand	345-346	2	5
β-strand	352-362	11	5
α-helix	365-367	3
β-strand	376-382	7	5
α-helix	383-385	3
β-strand	394-405	12	5
α-helix	407-409	3
β-strand	437	1	45
β-strand	438	1	24
α-helix	441-443	3
β-strand	448	1	45
β-strand	450-461	12	5
α-helix	501-507	7
α-helix	510-520	11
α-helix	525-531	7
α-helix	541-552	12
β-strand	557-558	2	46
β-strand	564-565	2	46
β-strand	571-575	5	47
α-helix	581-591	11
β-strand	595-599	5	47
β-strand	603	1	32
α-helix	605-608	4
β-strand	610-611	2	48
β-strand	624-625	2	48
α-helix	627-630	4
β-strand	635-639	5	47
α-helix	641-643	3
α-helix	646-658	13
β-strand	660-665	6	49
β-strand	668-673	6	49
β-strand	677-682	6	47
α-helix	695-698	4
α-helix	703-706	4
β-strand	711-715	5	47
α-helix	721-736	16
α-helix	748-758	11
β-strand	764-765	2	50
α-helix	767-784	18
α-helix	793-795	3
α-helix	797-813	17
β-strand	818-819	2	50
α-helix	821-832	12

Chain 7: 39 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	7-8	2	11
α-helix	14-27	14
α-helix	62-71	10
β-strand	77-81	5	51
α-helix	82-94	13
α-helix	102-108	7
α-helix	110-124	15
α-helix	125-128	4
α-helix	134-136	3
α-helix	138-153	16
α-helix	194-197	4
β-strand	201-205	5	51
α-helix	206-208	3
α-helix	220-222	3
β-strand	225	1	27
α-helix	232-234	3
β-strand	238-248	11	27
α-helix	249-251	3
β-strand	252-262	11	27
β-strand	267-272	6	27
β-strand	277-278	2	26
α-helix	279-281	3
α-helix	287-291	5
α-helix	296-297	2
β-strand	298-316	19	27
α-helix	319-321	3
α-helix	322-323	2
β-strand	330-336	7	27
α-helix	337-339	3
β-strand	348-358	11	27
α-helix	363-366	4
β-strand	371	1	11
β-strand	373-383	11	27
α-helix	388-390	3
α-helix	395-407	13
α-helix	410-417	8
α-helix	426-437	12
β-strand	442-443	2	52
β-strand	449-450	2	52
β-strand	456-460	5	53
α-helix	466-476	11
β-strand	480-484	5	53
β-strand	488	1	18
α-helix	490-494	5
β-strand	495-498	4	54
β-strand	507-510	4	54
α-helix	512-515	4
β-strand	520-524	5	53
α-helix	531-534	4
α-helix	537-543	7
β-strand	545-550	6	30
β-strand	553-558	6	30
β-strand	562-567	6	53
α-helix	568-569	2
α-helix	580-584	5
α-helix	588-591	4
β-strand	596-600	5	53
α-helix	606-622	17
α-helix	632-634	3
α-helix	635-645	11
β-strand	651	1	55
α-helix	654-674	21
α-helix	687-702	16
β-strand	707	1	55
α-helix	710-721	12
α-helix	722-728	7

Chain A: 15 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	56
α-helix	45-54	10
β-strand	69-71	3	57
α-helix	72-94	23
α-helix	99-113	15
α-helix	121-138	18
α-helix	142-162	21
α-helix	165-174	10
β-strand	182	1	58
α-helix	183-184	2
α-helix	185-190	6
α-helix	192-206	15
α-helix	212-221	10
β-strand	224-228	5	58
α-helix	229-232	4
α-helix	233-237	5
β-strand	242-244	3	58
β-strand	247-251	5	58
α-helix	256-276	21
α-helix	278-281	4
α-helix	288-296	9

Chain B: 31 helices, 39 β-strands

Element	Residues	Length	Sheet
α-helix	2-8	7
α-helix	13-16	4
α-helix	18-31	14
α-helix	35-46	12
α-helix	58-78	21
β-strand	205-207	3	59
α-helix	213-215	3
β-strand	236-238	3	60
α-helix	242-245	4
β-strand	249	1	61
α-helix	254-276	23
α-helix	280-282	3
β-strand	283	1	62
β-strand	294-302	9	62
α-helix	313-315	3
β-strand	316-319	4	62
α-helix	320-321	2
α-helix	322-325	4
β-strand	329-333	5	62
β-strand	340-342	3	63
β-strand	347-353	7	62
β-strand	359-366	8	62
α-helix	367-371	5
β-strand	372	1	64
α-helix	373-374	2
β-strand	375-377	3	65
α-helix	378-388	11
α-helix	391-392	2
β-strand	393-399	7	59
α-helix	411-419	9
α-helix	420-424	5
β-strand	428-432	5	59
β-strand	437	1	66
α-helix	441-445	5
α-helix	448-450	3
α-helix	461-473	13
β-strand	481-485	5	59
β-strand	490	1	66
β-strand	498	1	67
β-strand	500	1	68
α-helix	501-503	3
β-strand	515-517	3	59
β-strand	520	1	68
β-strand	522-526	5	60
β-strand	529-533	5	60
α-helix	538-541	4
β-strand	545-547	3	63
α-helix	549-553	5
α-helix	556-567	12
β-strand	569	1	69
α-helix	576-577	2
β-strand	578-581	4	70
β-strand	605-609	5	70
β-strand	612	1	67
α-helix	614-620	7
β-strand	621	1	69
α-helix	622	1
α-helix	623-625	3
β-strand	626	1	64
β-strand	630-632	3	60
β-strand	640-644	5	60
β-strand	647-651	5	60
β-strand	656	1	71
β-strand	662	1	71
β-strand	664-670	7	59
α-helix	671-673	3
β-strand	681-683	3	65
β-strand	689-691	3	65
α-helix	694-697	4
β-strand	698-704	7	59

13 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2	protein	868	Saccharomyces cerevisiae	P29469 (AlphaFold model)
DNA replication licensing factor MCM3	3	protein	1009	Saccharomyces cerevisiae	P24279 (AlphaFold model)
DNA replication licensing factor MCM4	4	protein	933	Saccharomyces cerevisiae	P30665 (AlphaFold model)
Minichromosome maintenance protein 5	5	protein	775	Saccharomyces cerevisiae	P29496 (AlphaFold model)
DNA replication licensing factor MCM6	6	protein	1017	Saccharomyces cerevisiae	P53091
DNA replication licensing factor MCM7	7	protein	845	Saccharomyces cerevisiae	P38132
DNA primase large subunit	A	protein	528	Saccharomyces cerevisiae	P20457
DNA polymerase alpha subunit B	B	protein	705	Saccharomyces cerevisiae	P38121
DNA replication complex GINS protein PSF1	C	protein	208	Saccharomyces cerevisiae	Q12488
DNA replication complex GINS protein PSF2	D	protein	213	Saccharomyces cerevisiae	P40359
DNA replication complex GINS protein PSF3	E	protein	217	Saccharomyces cerevisiae	Q12146
DNA replication complex GINS protein SLD5	F	protein	294	Saccharomyces cerevisiae	Q03406

9 more molecules are not listed.

Sequence of entity 1 (2), FASTA

>8B9A_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 2 (3), FASTA

>8B9A_2 DNA replication licensing factor MCM3 (chains 3)
MKRRWKKNFIAVSAANRFKKISSSGALENLYFQGEAPVMEGSTGFDGDATTFFAPDAVFG
DRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKK
AASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHP
NASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHY
AAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMP
EMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLIL
GNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAIL
LMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGL
TAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGI
HTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSIS
EHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVF
EKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKN
YTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDI
GNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSA
RRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGP
LTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEE
SYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV

Sequence of entity 3 (4), FASTA

>8B9A_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 4 (5), FASTA

>8B9A_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 5 (6), FASTA

>8B9A_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Sequence of entity 6 (7), FASTA

>8B9A_6 DNA replication licensing factor MCM7 (chains 7)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA

Sequence of entity 7 (A), FASTA

>8B9A_7 DNA primase large subunit (chains A)
MFRQSKRRIASRKNFSSYDDIVKSELDVGNTNAANQIILSSSSSEEEKKLYARLYESKLS
FYDLPPQGEITLEQFEIWAIDRLKILLEIESCLSRNKSIKEIETIIKPQFQKLLPFNTES
LEDRKKDYYSHFILRLCFCRSKELREKFVRAETFLFKIRFNMLTSTDQTKFVQSLDLPLL
QFISNEEKAELSHQLYQTVSASLQFQLNLNEEHQRKQYFQQEKFIKLPFENVIELVGNRL
VFLKDGYAYLPQFQQLNLLSNEFASKLNQELIKTYQYLPRLNEDDRLLPILNHLSSGYTI
ADFNQQKANQFSENVDDEINAQSVWSEEISSNYPLCIKNLMEGLKKNHHLRYYGRQQLSL
FLKGIGLSADEALKFWSEAFTRNGNMTMEKFNKEYRYSFRHNYGLEGNRINYKPWDCHTI
LSKPRPGRGDYHGCPFRDWSHERLSAELRSMKLTQAQIISVLDSCQKGEYTIACTKVFEM
THNSASADLEIGEQTHIAHPNLYFERSRQLQKKQQKLEKEKLFNNGNH

Sequence of entity 8 (B), FASTA

>8B9A_8 DNA polymerase alpha subunit B (chains B)
MSGSIDVITHFGPDADKPEIITALENLTKLHALSVEDLYIKWEQFSNQRRQTHTDLTSKN
IDEFKQFLQLQMEKRANQISSSSKVNTSTKKPVIKKSLNSSPLFGLSIPKTPTLKKRKLH
GPFSLSDSKQTYNVGSEAETNEKGNSSLKLEFTPGMAEDAVGDSAPLSHAKSSDAKTPGS
STFQTPTTNTPTTSRQNVPAGEILDSLNPENIEISSGNPNVGLLSTEEPSYNQVKVEPFY
DAKKYKFRTMRQNLQEASDVLDDQIESFTKIIQNHYKLSPNDFADPTIQSQSEIYAVGRI
VPDSPTYDKFLNPESLSLETSRMGGVGRRVRLDLSQVNELSFFLGQIVAFKGKNANGDYF
TVNSILPLPYPNSPVSTSQELQEFQANLEGSSLKVIVTCGPYFANDNFSLELLQEFIDSI
NNEVKPHVLIMFGPFIDITHPLIASGKLPNFPQFKTQPKTLDELFLKLFTPILKTISPHI
QTVLIPSTKDAISNHAAYPQASLIRKALQLPKRNFKCMANPSSFQINEIYFGCSNVDTFK
DLKEVIKGGTTSSRYRLDRVSEHILQQRRYYPIFPGSIRTRIKPKDVSTKKETNDMESKE
EKVYEHISGADLDVSYLGLTEFVGGFSPDIMIIPSELQHFARVVQNVVVINPGRFIRATG
NRGSYAQITVQCPDLEDGKLTLVEGEEPVYLHNVWKRARVDLIAS

Sequence of entity 9 (C), FASTA

>8B9A_9 DNA replication complex GINS protein PSF1 (chains C)
MYGDLGNKLVLEAKRTKQLYARSNQDVNLPMYHEDIIRNILKEVSNLRKNTEYLKEQQQL
GMLDDKVAKCQYFVTLLCMERNKRCLLAYQRLRTDILDSMAWNNNGLDLMSSITFSQQDT
NNLSHQEQEYLKEYCDLITDLKSGDLVDIDLSGSLVPPSDVFIDVRVLKDAGEIQTEYGV
FNLIKDSQFFVRQSDVERLIQQGYLQKI

Sequence of entity 10 (D), FASTA

>8B9A_10 DNA replication complex GINS protein PSF2 (chains D)
MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGTGNHTRWQLITTDDKAL
NNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKIQYEKTHPDRFSELPWNWL
VLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGLKYLNESHLQLDNLSLLEINE
LRPFITEIMDKLREIHTASLTAGTENDEEEFNI

Sequence of entity 11 (E), FASTA

>8B9A_11 DNA replication complex GINS protein PSF3 (chains E)
MGSSHHHHHHSSGLVPRGSHMASMGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRP
ITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDL
HSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSAN
TNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK

Sequence of entity 12 (F), FASTA

>8B9A_12 DNA replication complex GINS protein SLD5 (chains F)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	4
MG	Magnesium ion	Mg	4
ANP	Phosphoaminophosphonic acid-adenylate ester	C10 H17 N6 O12 P3	4

Primary citation

How Pol alpha-primase is targeted to replisomes to prime eukaryotic DNA replication. Jones, M.L., Aria, V., Baris, Y. et al. Mol Cell (2023) 83:2911. DOI 10.1016/j.molcel.2023.06.035 · PubMed

Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
8KG6 3.07 Å, Yeast replisome in state I
9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD

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