8D6A: Leukemia inhibitory factor

Cryo-EM structure of human LIF signaling complex: model containing the interaction core region. Determined by electron microscopy at 3.54 Å resolution. Released 29 Mar 2023.

Method
Electron microscopy
Resolution
3.54 Å
Organism
Homo sapiens
Chains
3
Atoms
7,912
Mol. weight
186.95 kDa
Ligands
NAG
Released
29 Mar 2023

Explore 8D6A in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8D6A contains 22 α-helices and 75 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 34 β-strands

ElementResiduesLengthSheet
α-helix125-1284
β-strand134-13521
β-strand136-13722
β-strand143-14421
β-strand159-16023
β-strand16114
α-helix170-1712
β-strand172-17323
α-helix187-1882
β-strand194-19635
β-strand19714
β-strand199-20026
β-strand207-20826
β-strand212-21435
β-strand220-22122
α-helix222-2254
β-strand226-23277
α-helix2331
β-strand240-24567
α-helix248-2514
β-strand255-26398
β-strand270-27128
α-helix272-2732
β-strand283-28647
β-strand295-30398
α-helix309-3135
β-strand317-31828
β-strand332-33659
β-strand345-35179
α-helix352-3554
β-strand365-370610
β-strand378-380310
β-strand387-39159
β-strand396-403810
β-strand414-416310
β-strand431-433311
β-strand440-442311
β-strand452-459812
β-strand469-473512
β-strand479-481311
β-strand492-500912
β-strand503-504212
β-strand508-512512
Chain B: 7 helices, 41 β-strands
ElementResiduesLengthSheet
β-strand133-134213
α-helix136-1394
β-strand143-147514
β-strand152-158714
β-strand169-1781015
β-strand185-192815
β-strand194116
β-strand197116
β-strand200-206714
β-strand216-2251015
β-strand232-233213
α-helix235-2417
β-strand242-244315
β-strand254-255217
β-strand259-262418
β-strand267-271517
β-strand279-281318
β-strand290-291217
β-strand297-301517
α-helix3111
β-strand312-317618
β-strand322-330918
α-helix331-3366
β-strand337-340419
β-strand343120
β-strand348-350321
β-strand352119
β-strand353122
β-strand354119
β-strand368-373623
β-strand379-381323
β-strand393122
β-strand396-398321
α-helix399-4002
β-strand406-413823
β-strand418-425823
β-strand431120
α-helix433-4364
β-strand437-440424
β-strand452-454324
β-strand464125
β-strand465-471726
β-strand477-481526
β-strand484125
β-strand491124
β-strand506-512726
α-helix518-5225
β-strand526126
Chain D: 6 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix44-6926
α-helix73-764
α-helix78-814
α-helix98-12629
α-helix132-15827
α-helix177-20125

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Leukemia inhibitory factorDprotein179Homo sapiensP15018 (AlphaFold model)
Interleukin-6 receptor subunit betaAprotein625Homo sapiensP40189 (AlphaFold model)
Leukemia inhibitory factor receptorBprotein817Homo sapiensP42702 (AlphaFold model)
Sequence of entity 1 (D), FASTA
>8D6A_1 Leukemia inhibitory factor (chains D)
PLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCG
PNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADI
LRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
Sequence of entity 2 (A), FASTA
>8D6A_2 Interleukin-6 receptor subunit beta (chains A)
ELLDPCGYISPESPVVQLHSNFTAVCVLKEKCMDYFHVNANYIVWKTNHFTIPKEQYTII
NRTASSVTFTDIASLNIQLTCNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGKKM
RCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAEN
ALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYR
TKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITY
EDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVTLTRWKSHLQNYTV
NATKLTVNLTNDRYLATLTVRNLVGKSDAAVLTIPACDFQATHPVMDLKAFPKDNMLWVE
WTTPRESVKKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESKCYLITVTPVYAD
GPGSPESIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRTII
GNETAVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIEEQK
LISEEDLGGEQKLISEEDLHHHHHH
Sequence of entity 3 (B), FASTA
>8D6A_3 Leukemia inhibitory factor receptor (chains B)
QKKGAPHDLKCVTNNLQVWNCSWKAPSGTGRGTDYEVCIENRSRSCYQLEKTSIKIPALS
HGDYEITINSLHDFGSSTSKFTLNEQNVSLIPDTPEILNLSADFSTSTLYLKWNDRGSVF
PHRSNVIWEIKVLRKESMELVKLVTHNTTLNGKDTLHHWSWASDMPLECAIHFVEIRCYI
DNLHFSGLEEWSDWSPVKNISWIPDSQTKVFPQDKVILVGSDITFCCVSQEKVLSALIGH
TNCPLIHLDGENVAIKIRNISVSASSGTNVVFTTEDNIFGTVIFAGYPPDTPQQLNCETH
DLKEIICSWNPGRVTALVGPRATSYTLVESFSGKYVRLKRAEAPTNESYQLLFQMLPNQE
IYNFTLNAHNPLGRSQSTILVNITEKVYPHTPTSFKVKDINSTAVKLSWHLPGNFAKINF
LCEIEIKKSNSVQEQRNVTIKGVENSSYLVALDKLNPYTLYTFRIRCSTETFWKWSKWSN
KKQHLTTEASPSKGPDTWREWSSDGKNLIIYWKPLPINEANGKILSYNVSCSSDEETQSL
SEIPDPQHKAEIRLDKNDYIISVVAKNSVGSSPPSKIASMEIPNDDLKIEQVVGMGKGIL
LTWHYDPNMTCDYVIKWCNSSRSEPCLMDWRKVPSNSTETVIESDEFRPGIRYNFFLYGC
RNQGYQLLRSMIGYIEELAPIVAPNFTVEDTSADSILVKWEDIPVEELRGFLRGYLFYFG
KGERDTSKMRVLESGRSDIKVKNITDISQKTLRIADLQGKTSYHLVLRAYTDGGVGPEKS
MYVVTKENSEQKLISEEDLGGEQKLISEEDLHHHHHH

Ligands and cofactors

IDNameFormulaCopies
NAG2-acetamido-2-deoxy-beta-D-glucopyranoseC8 H15 N O65

Primary citation

Structural insights into the assembly of gp130 family cytokine signaling complexes. Zhou, Y., Stevis, P.E., Cao, J. et al. Sci Adv (2023) 9:eade4395-eade4395. DOI 10.1126/sciadv.ade4395 · PubMed

Other PDB entries of the same protein (UniProt P15018 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 8D6A directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.