8GY7: ACTH-bound melanocortin-2 receptor
Cryo-EM structure of ACTH-bound melanocortin-2 receptor in complex with MRAP1 and Gs protein. Determined by electron microscopy at 3.3 Å resolution. Released 11 Jan 2023.
- Method
- Electron microscopy
- Resolution
- 3.3 Å
- Organisms
- Homo sapiens, synthetic construct
- Chains
- 6
- Atoms
- 7,661
- Mol. weight
- 180.14 kDa
- Ligands
- CA
- Released
- 11 Jan 2023
Explore 8GY7 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8GY7 contains 33 α-helices and 40 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-31 | 25 | |
| β-strand | 33-34 | 2 | 1 |
| β-strand | 36-40 | 5 | 2 |
| α-helix | 46-52 | 7 | |
| β-strand | 185-186 | 2 | 2 |
| β-strand | 189-190 | 2 | 1 |
| β-strand | 195-196 | 2 | 1 |
| β-strand | 199-200 | 2 | 2 |
| α-helix | 213-215 | 3 | |
| β-strand | 220-226 | 7 | 2 |
| α-helix | 233-244 | 12 | |
| β-strand | 253-259 | 7 | 2 |
| α-helix | 261-269 | 9 | |
| α-helix | 275-277 | 3 | |
| α-helix | 280-284 | 5 | |
| α-helix | 293-294 | 2 | |
| α-helix | 299-317 | 19 | |
| β-strand | 326-330 | 5 | 2 |
| α-helix | 338-358 | 21 | |
Chain B: 5 helices, 29 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-29 | 21 | |
| α-helix | 35-40 | 6 | |
| α-helix | 43-44 | 2 | |
| β-strand | 51-56 | 6 | 3 |
| β-strand | 63-68 | 6 | 4 |
| β-strand | 74-79 | 6 | 4 |
| β-strand | 83-88 | 6 | 4 |
| β-strand | 94-99 | 6 | 4 |
| β-strand | 105-110 | 6 | 5 |
| β-strand | 116-121 | 6 | 5 |
| β-strand | 125-130 | 6 | 5 |
| β-strand | 139-145 | 7 | 5 |
| β-strand | 151-158 | 8 | 6 |
| β-strand | 161-166 | 6 | 6 |
| β-strand | 169-175 | 7 | 6 |
| β-strand | 180-186 | 7 | 6 |
| β-strand | 192-197 | 6 | 7 |
| β-strand | 203-208 | 6 | 7 |
| β-strand | 212-217 | 6 | 7 |
| β-strand | 223-228 | 6 | 7 |
| β-strand | 234-239 | 6 | 8 |
| β-strand | 245-250 | 6 | 8 |
| β-strand | 255-259 | 5 | 8 |
| β-strand | 264-269 | 6 | 8 |
| α-helix | 277 | 1 | |
| β-strand | 278-283 | 6 | 9 |
| α-helix | 284 | 1 | |
| β-strand | 289-294 | 6 | 9 |
| β-strand | 299-303 | 5 | 9 |
| β-strand | 311-313 | 3 | 9 |
| β-strand | 320-322 | 3 | 3 |
| β-strand | 325 | 1 | 3 |
| β-strand | 332-336 | 5 | 3 |
| β-strand | 341-345 | 5 | 3 |
Chain G: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-23 | 14 | |
| α-helix | 30-43 | 14 | |
| α-helix | 53-55 | 3 | |
Chain M: 0 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| β-strand | 13-15 | 3 | 10 |
Chain P: 2 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| β-strand | 18-20 | 3 | 10 |
| α-helix | 21-24 | 4 | |
| α-helix | 37-62 | 26 | |
Chain R: 13 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 28-48 | 21 | |
| α-helix | 58-87 | 30 | |
| α-helix | 97-133 | 37 | |
| α-helix | 135-137 | 3 | |
| α-helix | 138-141 | 4 | |
| α-helix | 144-167 | 24 | |
| α-helix | 172-207 | 36 | |
| α-helix | 216-230 | 15 | |
| α-helix | 233-244 | 12 | |
| α-helix | 249-255 | 7 | |
| α-helix | 258-271 | 14 | |
| α-helix | 273-276 | 4 | |
| α-helix | 281-290 | 10 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(i) subunit alpha-3,Guanine nucleotide-binding protein G(s)… | A | protein | 361 | Homo sapiens | P08754 (AlphaFold model), Q5JWF2 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 371 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| Corticotropin | M | protein | 18 | synthetic construct | P68000 |
| Melanocortin-2 receptor accessory protein | P | protein | 184 | Homo sapiens | Q8TCY5 |
| Soluble cytochrome b562,Adrenocorticotropic hormone receptor,melanocortin-2… | R | protein | 601 | Homo sapiens | P0ABE7, Q01718 |
Sequence of entity 1 (A), FASTA
>8GY7_1 Guanine nucleotide-binding protein G(i) subunit alpha-3,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L
Sequence of entity 2 (B), FASTA
>8GY7_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSG
VSGWRLFKKIS
Sequence of entity 3 (G), FASTA
>8GY7_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 4 (M), FASTA
>8GY7_4 Corticotropin (chains M)
SYSMEHFRWGKPVGKKRR
Sequence of entity 5 (P), FASTA
>8GY7_5 Melanocortin-2 receptor accessory protein (chains P)
MDYKDDDDKGSGMANGTNASAPYYSYEYYLDYLDLIPVDEKKLKAHKHSIVIAFWVSLAA
FVVLLFLILLYMSWSASPQMRNSPKHHQTCPWSHGLNLHLCIQKCLPCHREPLATSQAQA
SSVEPGSRTGPDQPLRQESSSTLPLGGFQTHPTLLWELTLNGGPLVRSKPSEPPPGDRTS
QLQS
Sequence of entity 6 (R), FASTA
>8GY7_6 Soluble cytochrome b562,Adrenocorticotropic hormone receptor,melanocortin-2 receptor,Adrenocorticotropic hormone receptor,Adrenocorticotropic hormone receptor (chains R)
MKTIIALSYIFCLVFAHHHHHHHHHHADLEDNWETLNDNLKVIEKADNAAQVKDALTKMR
AAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQL
KTTRNAYIQKYLKHIINSYENINNTARNNSDCPRVVLPEEIFFTISIVGVLENLIVLLAV
FKNKNLQAPMYFFICSLAISDMLGSLYKILENILIILRNMGYLKPRGSFETTADDIIDSL
FVLSLLGSIFSLSVIAADRYITIFHALRYHSIVTMRRTVVVLTVIWTFCTGTGITMVIFS
HHVPTVITFTSLFPLMLVFILCLYVHMFLLARSHTRKISTLPRANMKGAITLTILLGVFI
FCWAPFVLHVLLMTFCPSNPYCACYMSLFQVNGMLIMCNAVIDPFIYAFRSPELRDAFKK
MIFCSRYWGSSGGGGSGGGGSSGVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAV
SVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGT
LVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTIN
S
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CA | Calcium ion | Ca | 1 |
Primary citation
Structural basis of signaling regulation of the human melanocortin-2 receptor by MRAP1. Luo, P., Feng, W., Ma, S. et al. Cell Res (2023) 33:46-54. DOI 10.1038/s41422-022-00751-6 · PubMed
Other PDB entries of the same protein (UniProt P08754 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 2ODE 1.9 Å, Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3
- 9YCX 2.1 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the…
- 9YCY 2.2 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the Canonical…
- 8IKL 2.33 Å, Cryo-EM structure of the CD97-G13 complex
- 8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
- 7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
- 9VJ6 2.62 Å, Cryo-EM structure of 5-HT1AR-Gi3 in complex with F-15599
- 7T11 2.7 Å, CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
- 9VJF 2.7 Å, Cryo-EM structure of 5-HT1AR-Gi3 in complex with buspirone
- 2IHB 2.71 Å, Crystal structure of the heterodimeric complex of human RGS10 and activated Gi alpha 3
- 2V4Z 2.8 Å, The crystal structure of the human G-protein subunit alpha (GNAI3) in complex with an…
- 7KH0 2.8 Å, Cryo-EM structure of the human arginine vasopressin AVP-vasopressin receptor V2R-Gs…
Browse structure collections
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