8ID4: Linoleic acid bound GPR120-Gi complex
Cryo-EM structure of the linoleic acid bound GPR120-Gi complex. Determined by electron microscopy at 3.1 Å resolution. Released 15 Mar 2023.
- Method
- Electron microscopy
- Resolution
- 3.1 Å
- Organism
- Homo sapiens
- Chains
- 5
- Atoms
- 8,665
- Mol. weight
- 156.74 kDa
- Ligands
- EIC
- Released
- 15 Mar 2023
Explore 8ID4 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8ID4 contains 36 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 14 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-29 | 23 | |
| α-helix | 30-32 | 3 | |
| β-strand | 34-40 | 7 | 2 |
| α-helix | 46-52 | 7 | |
| β-strand | 185-190 | 6 | 2 |
| β-strand | 195-200 | 6 | 2 |
| α-helix | 208-210 | 3 | |
| α-helix | 212-215 | 4 | |
| β-strand | 220-226 | 7 | 2 |
| α-helix | 227-230 | 4 | |
| α-helix | 232 | 1 | |
| β-strand | 233 | 1 | 3 |
| α-helix | 234 | 1 | |
| β-strand | 241 | 1 | 3 |
| α-helix | 242-254 | 13 | |
| β-strand | 263-269 | 7 | 2 |
| α-helix | 271-273 | 3 | |
| α-helix | 274-277 | 4 | |
| α-helix | 283-285 | 3 | |
| α-helix | 296-308 | 13 | |
| β-strand | 319-322 | 4 | 2 |
| α-helix | 330-350 | 21 | |
Chain B: 5 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-23 | 21 | |
| α-helix | 30-33 | 4 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-52 | 6 | 4 |
| β-strand | 58-63 | 6 | 5 |
| β-strand | 70-74 | 5 | 5 |
| β-strand | 78-82 | 5 | 5 |
| β-strand | 89-94 | 6 | 5 |
| α-helix | 95 | 1 | |
| β-strand | 100-105 | 6 | 6 |
| β-strand | 111-116 | 6 | 6 |
| β-strand | 120-125 | 6 | 6 |
| β-strand | 134-140 | 7 | 6 |
| β-strand | 146-153 | 8 | 7 |
| β-strand | 156-161 | 6 | 7 |
| β-strand | 165-170 | 6 | 7 |
| β-strand | 175-181 | 7 | 7 |
| β-strand | 187-192 | 6 | 8 |
| β-strand | 198-203 | 6 | 8 |
| β-strand | 207-212 | 6 | 8 |
| β-strand | 218-223 | 6 | 8 |
| β-strand | 229-234 | 6 | 9 |
| β-strand | 240-245 | 6 | 9 |
| β-strand | 250-254 | 5 | 9 |
| β-strand | 259-264 | 6 | 9 |
| α-helix | 272 | 1 | |
| β-strand | 273-278 | 6 | 10 |
| β-strand | 284-289 | 6 | 10 |
| β-strand | 294-298 | 5 | 10 |
| β-strand | 304-308 | 5 | 10 |
| β-strand | 315-320 | 6 | 4 |
| β-strand | 327-331 | 5 | 4 |
| β-strand | 335-339 | 5 | 4 |
Chain R: 12 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 39-68 | 30 | |
| α-helix | 78-89 | 12 | |
| α-helix | 92-100 | 9 | |
| α-helix | 110-112 | 3 | |
| α-helix | 114-141 | 28 | |
| α-helix | 152-170 | 19 | |
| α-helix | 171-173 | 3 | |
| β-strand | 177-181 | 5 | 1 |
| β-strand | 192-196 | 5 | 1 |
| α-helix | 202-212 | 11 | |
| α-helix | 213-217 | 5 | |
| α-helix | 218-243 | 26 | |
| α-helix | 252-293 | 42 | |
| α-helix | 302-320 | 19 | |
Chain S: 3 helices, 25 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 11 |
| β-strand | 10-12 | 3 | 12 |
| β-strand | 17-25 | 9 | 11 |
| α-helix | 29-31 | 3 | |
| β-strand | 33-39 | 7 | 13 |
| β-strand | 45-51 | 7 | 13 |
| β-strand | 58-60 | 3 | 13 |
| β-strand | 68-73 | 6 | 11 |
| β-strand | 78-84 | 7 | 11 |
| β-strand | 92-94 | 3 | 12 |
| β-strand | 95-99 | 5 | 13 |
| β-strand | 111 | 1 | 13 |
| β-strand | 115-119 | 5 | 12 |
| β-strand | 141-142 | 2 | 14 |
| β-strand | 147-149 | 3 | 15 |
| β-strand | 156-162 | 7 | 14 |
| β-strand | 167 | 1 | 16 |
| β-strand | 173 | 1 | 16 |
| β-strand | 175-180 | 6 | 15 |
| β-strand | 187-191 | 5 | 15 |
| β-strand | 195-196 | 2 | 15 |
| α-helix | 197 | 1 | |
| β-strand | 204-209 | 6 | 14 |
| β-strand | 212-217 | 6 | 14 |
| β-strand | 226-232 | 7 | 15 |
| α-helix | 238 | 1 | |
| β-strand | 240 | 1 | 15 |
| β-strand | 244-247 | 4 | 15 |
Chain Y: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-23 | 15 | |
| α-helix | 30-43 | 14 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Free fatty acid receptor 4 | R | protein | 361 | Homo sapiens | Q5NUL3 (AlphaFold model) |
| Guanine nucleotide-binding protein G(i) subunit alpha-1 | A | protein | 354 | Homo sapiens | P63096 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 339 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | Y | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| scFv16 | S | protein | 285 | Homo sapiens | |
Sequence of entity 1 (R), FASTA
>8ID4_1 Free fatty acid receptor 4 (chains R)
MSPECARAAGDAPLRSLEQANRTRFPFFSDVKGDHRLVLAAVETTVLVLIFAVSLLGNVC
ALVLVARRRRRGATACLVLNLFCADLLFISAIPLVLAVRWTEAWLLGPVACHLLFYVMTL
SGSVTILTLAAVSLERMVCIVHLQRGVRGPGRRARAVLLALIWGYSAVAALPLCVFFRVV
PQRLPGADQEISICTLIWPTIPGEISWDVSFVTLNFLVPGLVIVISYSKILQITKASRKR
LTVSLAYSESHQIRVSQQDFRLFRTLFLLMVSFFIMWSPIIITILLILIQNFKQDLVIWP
SLFFWVVAFTFANSALNPILYNMTLCRNEWKKIFCCFWFPEKGAILTDTSVKRNDLSIIS
G
Sequence of entity 2 (A), FASTA
>8ID4_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Sequence of entity 3 (B), FASTA
>8ID4_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAM
HWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNIC
SIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFT
GHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAF
ATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALK
ADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 4 (Y), FASTA
>8ID4_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains Y)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 5 (S), FASTA
>8ID4_5 scFv16 (chains S)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFAVQLVESGGGLVQPGGSRKLSCS
ASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQM
TSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSAGGGGSGGGGSGGGGSADIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLEL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| EIC | Linoleic acid | C18 H32 O2 | 1 |
Primary citation
Unsaturated bond recognition leads to biased signal in a fatty acid receptor. Mao, C., Xiao, P., Tao, X.N. et al. Science (2023) 380:eadd6220-eadd6220. DOI 10.1126/science.add6220 · PubMed
Other PDB entries of the same protein (UniProt Q5NUL3 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8G59 2.64 Å, Cryo-EM structure of the TUG891 bound GPR120-Giq complex
- 24XB 2.65 Å, Cryo-EM Structure of the Apo FFAR4-Gq Complex
- 24QH 2.74 Å, Cryo-EM Structure of the FFAR4-Gi complex with unknown density
- 8ID6 2.8 Å, Cryo-EM structure of the oleic acid bound GPR120-Gi complex
- 8IYS 2.95 Å, TUG891-bound FFAR4 in complex with Gq
- 9XFI 2.99 Å, Cryo-EM structure of the Bavachalcone bound FFAR4-Giq complex
- 8ID8 3.0 Å, Cryo-EM structure of the TUG891 bound GPR120-Gi complex
- 8ID9 3.0 Å, Cryo-EM structure of the eicosapentaenoic acid bound GPR120-Gi complex
- 8H4I 3.06 Å, DHA-bound FFAR4 in complex with Gs
- 8T3O 3.06 Å, Cryo-EM structure of the TUG-891 bound FFA4-Gq complex
- 8H4L 3.07 Å, DHA-bound FFAR4 in complex with Gq
- 8H4K 3.1 Å, GW9508-bound FFAR4 in complex with Gq
Browse structure collections
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